A common pattern of DNase I footprinting throughout the human mtDNA unveils clues for a chromatin-like organization

• Published in: Genome research Vol. 28; no. 8; pp. 1158 - 1168
• Main Authors: Blumberg, Amit, Danko, Charles G, Kundaje, Anshul, Mishmar, Dan
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.08.2018
• Subjects: Animals; CD34 antigen; Cell Line; Chromatin; Chromatin - genetics; Deoxyribonuclease; Deoxyribonucleases - genetics; Deoxyribonucleic acid; DNA; DNA Footprinting - methods; DNA, Mitochondrial - genetics; DNA-Binding Proteins - genetics; Fetuses; Footprinting; G-Quadruplexes; Gene Expression Regulation; Genome, Human; HeLa Cells; Histones; Humans; Interleukin 3; Mice; Mitochondria - genetics; Mitochondrial DNA; Mitochondrial Proteins - genetics; Regulatory sequences; Synteny; Transcription Factors - genetics

Human mitochondrial DNA (mtDNA) is believed to lack chromatin and histones. Instead, it is coated solely by the transcription factor TFAM. We asked whether mtDNA packaging is more regulated than once thought. To address this, we analyzed DNase-seq experiments in 324 human cell types and found, for t...