mTORC1 activity is supported by spatial association with focal adhesions
The mammalian target of rapamycin complex 1 (mTORC1) integrates mitogenic and stress signals to control growth and metabolism. Activation of mTORC1 by amino acids and growth factors involves recruitment of the complex to the lysosomal membrane and is further supported by lysosome distribution to the...
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| Published in | The Journal of cell biology Vol. 220; no. 5; p. 1 |
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| Main Authors | , , , , , , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Rockefeller University Press
03.05.2021
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| Subjects | |
| Online Access | Get full text |
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| Abstract | The mammalian target of rapamycin complex 1 (mTORC1) integrates mitogenic and stress signals to control growth and metabolism. Activation of mTORC1 by amino acids and growth factors involves recruitment of the complex to the lysosomal membrane and is further supported by lysosome distribution to the cell periphery. Here, we show that translocation of lysosomes toward the cell periphery brings mTORC1 into proximity with focal adhesions (FAs). We demonstrate that FAs constitute discrete plasma membrane hubs mediating growth factor signaling and amino acid input into the cell. FAs, as well as the translocation of lysosome-bound mTORC1 to their vicinity, contribute to both peripheral and intracellular mTORC1 activity. Conversely, lysosomal distribution to the cell periphery is dispensable for the activation of mTORC1 constitutively targeted to FAs. This study advances our understanding of spatial mTORC1 regulation by demonstrating that the localization of mTORC1 to FAs is both necessary and sufficient for its activation by growth-promoting stimuli. |
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| AbstractList | The mammalian target of rapamycin complex 1 (mTORC1) integrates mitogenic and stress signals to control growth and metabolism. Activation of mTORC1 by amino acids and growth factors involves recruitment of the complex to the lysosomal membrane and is further supported by lysosome distribution to the cell periphery. Here, we show that translocation of lysosomes toward the cell periphery brings mTORC1 into proximity with focal adhesions (FAs). We demonstrate that FAs constitute discrete plasma membrane hubs mediating growth factor signaling and amino acid input into the cell. FAs, as well as the translocation of lysosome-bound mTORC1 to their vicinity, contribute to both peripheral and intracellular mTORC1 activity. Conversely, lysosomal distribution to the cell periphery is dispensable for the activation of mTORC1 constitutively targeted to FAs. This study advances our understanding of spatial mTORC1 regulation by demonstrating that the localization of mTORC1 to FAs is both necessary and sufficient for its activation by growth-promoting stimuli. Rabanal-Ruiz and Byron et al. present a novel mechanism of nutrient signaling that identifies FAs as key cellular hubs that coordinate growth factor signaling and amino acid input into the cell and are required for efficient downstream activation of mTORC1. The mammalian target of rapamycin complex 1 (mTORC1) integrates mitogenic and stress signals to control growth and metabolism. Activation of mTORC1 by amino acids and growth factors involves recruitment of the complex to the lysosomal membrane and is further supported by lysosome distribution to the cell periphery. Here, we show that translocation of lysosomes toward the cell periphery brings mTORC1 into proximity with focal adhesions (FAs). We demonstrate that FAs constitute discrete plasma membrane hubs mediating growth factor signaling and amino acid input into the cell. FAs, as well as the translocation of lysosome-bound mTORC1 to their vicinity, contribute to both peripheral and intracellular mTORC1 activity. Conversely, lysosomal distribution to the cell periphery is dispensable for the activation of mTORC1 constitutively targeted to FAs. This study advances our understanding of spatial mTORC1 regulation by demonstrating that the localization of mTORC1 to FAs is both necessary and sufficient for its activation by growth-promoting stimuli. |
| Author | Vanhaesebroeck, Bart Fässler, Reinhard Rabanal-Ruiz, Yoana Wills, Jimi C Maddocks, Oliver D K Madsen, Ralitsa Zeug, André Carroll, Bernadette Sedlackova, Lucia Byron, Adam Nelson, Glyn Stingele, Julian Korolchuk, Viktor I Ponimaskin, Evgeni Zhang, Tong Wirth, Alexander Hewitt, Graeme |
| AuthorAffiliation | 4 UCL Cancer Institute, University College London, London, UK 10 Institute of Neuroscience, Lobachevsky State University of Nizhni Novgorod, Nizhny Novgorod, Russia 1 Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK 9 Department of Molecular Medicine, Max Planck Institute of Biochemistry, Martinsried, Germany 11 School of Biochemistry, University of Bristol, Bristol, UK 2 Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK 6 Gene Center, Ludwig Maximilians University Munich, Munich, Germany 8 Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Glasgow, UK 5 DSB Repair Metabolism Laboratory, The Francis Crick Institute, London, UK 7 Department of Biochemistry, Ludwig Maximilians University Munich, Munich, Germany 3 Cellular Neurophysiology, Hannover Medical School, Hannover, Germany |
| AuthorAffiliation_xml | – name: 7 Department of Biochemistry, Ludwig Maximilians University Munich, Munich, Germany – name: 1 Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK – name: 5 DSB Repair Metabolism Laboratory, The Francis Crick Institute, London, UK – name: 3 Cellular Neurophysiology, Hannover Medical School, Hannover, Germany – name: 10 Institute of Neuroscience, Lobachevsky State University of Nizhni Novgorod, Nizhny Novgorod, Russia – name: 2 Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK – name: 4 UCL Cancer Institute, University College London, London, UK – name: 6 Gene Center, Ludwig Maximilians University Munich, Munich, Germany – name: 8 Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Glasgow, UK – name: 9 Department of Molecular Medicine, Max Planck Institute of Biochemistry, Martinsried, Germany – name: 11 School of Biochemistry, University of Bristol, Bristol, UK |
| Author_xml | – sequence: 1 givenname: Yoana surname: Rabanal-Ruiz fullname: Rabanal-Ruiz, Yoana organization: Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK – sequence: 2 givenname: Adam surname: Byron fullname: Byron, Adam organization: Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK – sequence: 3 givenname: Alexander surname: Wirth fullname: Wirth, Alexander organization: Cellular Neurophysiology, Hannover Medical School, Hannover, Germany – sequence: 4 givenname: Ralitsa surname: Madsen fullname: Madsen, Ralitsa organization: UCL Cancer Institute, University College London, London, UK – sequence: 5 givenname: Lucia surname: Sedlackova fullname: Sedlackova, Lucia organization: Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK – sequence: 6 givenname: Graeme surname: Hewitt fullname: Hewitt, Graeme organization: DSB Repair Metabolism Laboratory, The Francis Crick Institute, London, UK – sequence: 7 givenname: Glyn surname: Nelson fullname: Nelson, Glyn organization: Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK – sequence: 8 givenname: Julian surname: Stingele fullname: Stingele, Julian organization: Department of Biochemistry, Ludwig Maximilians University Munich, Munich, Germany – sequence: 9 givenname: Jimi C surname: Wills fullname: Wills, Jimi C organization: Cancer Research UK Edinburgh Centre, Institute of Genetics and Molecular Medicine, University of Edinburgh, Edinburgh, UK – sequence: 10 givenname: Tong surname: Zhang fullname: Zhang, Tong organization: Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Glasgow, UK – sequence: 11 givenname: André surname: Zeug fullname: Zeug, André organization: Cellular Neurophysiology, Hannover Medical School, Hannover, Germany – sequence: 12 givenname: Reinhard surname: Fässler fullname: Fässler, Reinhard organization: Department of Molecular Medicine, Max Planck Institute of Biochemistry, Martinsried, Germany – sequence: 13 givenname: Bart surname: Vanhaesebroeck fullname: Vanhaesebroeck, Bart organization: UCL Cancer Institute, University College London, London, UK – sequence: 14 givenname: Oliver D K surname: Maddocks fullname: Maddocks, Oliver D K organization: Wolfson Wohl Cancer Research Centre, Institute of Cancer Sciences, University of Glasgow, Glasgow, UK – sequence: 15 givenname: Evgeni surname: Ponimaskin fullname: Ponimaskin, Evgeni organization: Institute of Neuroscience, Lobachevsky State University of Nizhni Novgorod, Nizhny Novgorod, Russia – sequence: 16 givenname: Bernadette surname: Carroll fullname: Carroll, Bernadette organization: School of Biochemistry, University of Bristol, Bristol, UK – sequence: 17 givenname: Viktor I surname: Korolchuk fullname: Korolchuk, Viktor I organization: Biosciences Institute, Faculty of Medical Sciences, Newcastle University, Newcastle upon Tyne, UK |
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| Cites_doi | 10.1093/nar/gky1106 10.1016/j.ceb.2016.02.013 10.1093/bioinformatics/bth078 10.12688/f1000research.4410.2 10.1083/jcb.202003038 10.1007/978-1-61779-166-6_19 10.1083/jcb.200302001 10.1186/1471-2105-11-395 10.1126/science.1177321 10.1126/scisignal.aaf3572 10.1038/nmeth.2557 10.1016/j.jmb.2016.11.027 10.1091/mbc.E15-12-0844 10.1242/jcs.00792 10.1093/nar/gki475 10.1080/15548627.2016.1212791 10.1093/bioinformatics/btr260 10.1038/s41580-019-0185-4 10.1038/ncb3257 10.1242/bio.027458 10.1038/nrm3769 10.1083/jcb.201610113 10.1038/nbt.1511 10.1073/pnas.1406459111 10.1016/j.celrep.2014.12.037 10.1038/nmeth.3901 10.1093/bioinformatics/bth349 10.1016/j.celrep.2015.02.031 10.1111/febs.14400 10.1016/j.cell.2017.02.004 10.1007/978-1-4939-7759-8_13 10.1080/15548627.2019.1580107 10.1073/pnas.0506580102 10.1016/j.cmet.2017.07.001 10.1083/jcb.201102090 10.1038/nbt.2377 10.3390/ijms19030818 10.1083/jcb.201104128 10.1093/nar/gkq537 10.1242/jcs.107.2.709 10.7554/eLife.11058 10.1016/j.celrep.2016.10.062 10.1016/j.molcel.2019.05.009 10.1038/ncb2204 10.1083/jcb.201503075 10.1038/nprot.2007.261 10.1021/pr101065j 10.1016/j.cell.2010.02.024 10.7554/eLife.10130 10.1038/ncb0402-e75 10.1038/onc.2012.512 10.15252/embr.201541382 10.1146/annurev-cellbio-092910-154017 10.1242/jcs.196287 10.1083/jcb.201310043 10.1101/gr.1239303 10.1083/jcb.201611073 10.1016/j.bbamcr.2015.06.004 |
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| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Y. Rabanal-Ruiz’s present address is Department of Medical Sciences, Faculty of Medicine, University of Castilla-la Mancha, Ciudad Real, Spain. Y. Rabanal-Ruiz and A. Byron contributed equally to this paper. Lucia Sedlackova’s present address is Centre for Genomic Regulation, The Barcelona Institute of Science and Technology, Barcelona, Spain. |
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| References | 33851967 - J Cell Biol. 2021 May 3;220(5) Chastney (2023072801095373600_bib10) 2020; 219 Eberwein (2023072801095373600_bib16) 2015; 1853 Pu (2023072801095373600_bib37) 2016; 129 Pluskal (2023072801095373600_bib36) 2010; 11 Cox (2023072801095373600_bib11) 2008; 26 Zhou (2023072801095373600_bib60) 2015; 10 Carroll (2023072801095373600_bib7) 2016; 5 Horton (2023072801095373600_bib22) 2015; 17 Yamada (2023072801095373600_bib57) 2002; 4 Guardia (2023072801095373600_bib19) 2016; 17 Theodosiou (2023072801095373600_bib48) 2016; 5 Kim (2023072801095373600_bib28) 2016; 27 Byron (2023072801095373600_bib5) 2016; 39 Ballabio (2023072801095373600_bib3) 2020; 21 Liberzon (2023072801095373600_bib31) 2011; 27 Sancak (2023072801095373600_bib42) 2010; 141 Schiefermeier (2023072801095373600_bib44) 2014; 205 Avivar-Valderas (2023072801095373600_bib2) 2013; 32 Rabanal-Ruiz (2023072801095373600_bib38) 2018; 19 Nersisyan (2023072801095373600_bib34) 2014; 3 Subramanian (2023072801095373600_bib47) 2005; 102 Cox (2023072801095373600_bib12) 2011; 10 Vlahakis (2023072801095373600_bib50) 2017; 429 Giannone (2023072801095373600_bib17) 2003; 163 Dong (2023072801095373600_bib15) 2016; 9 Warde-Farley (2023072801095373600_bib52) 2010; 38 Kenific (2023072801095373600_bib26) 2016; 212 Wolfson (2023072801095373600_bib54) 2017; 26 Perez-Riverol (2023072801095373600_bib35) 2019; 47 Rainero (2023072801095373600_bib39) 2015; 10 Saxton (2023072801095373600_bib43) 2017; 168 Ahel (2023072801095373600_bib1) 2009; 325 Xu (2023072801095373600_bib56) 2016; 12 Kim (2023072801095373600_bib27) 2014; 111 Carroll (2023072801095373600_bib6) 2018; 285 de Hoon (2023072801095373600_bib13) 2004; 20 Korolchuk (2023072801095373600_bib29) 2011; 13 Hong (2023072801095373600_bib21) 2017; 216 Wood (2023072801095373600_bib55) 1994; 107 Hatakeyama (2023072801095373600_bib20) 2019; 15 Winograd-Katz (2023072801095373600_bib53) 2014; 15 Kuo (2023072801095373600_bib30) 2012; 757 Tyanova (2023072801095373600_bib49) 2016; 13 Chambers (2023072801095373600_bib9) 2012; 30 Zhang (2023072801095373600_bib59) 2005; 33 Kawano (2023072801095373600_bib25) 2017; 6 Zaidel-Bar (2023072801095373600_bib58) 2003; 116 Rappsilber (2023072801095373600_bib40) 2007; 2 Wang (2023072801095373600_bib51) 2011; 195 Liu (2023072801095373600_bib32) 2011; 194 Starling (2023072801095373600_bib46) 2016; 17 Carroll (2023072801095373600_bib8) 2017; 216 Byron (2023072801095373600_bib4) 2018; 1764 Shannon (2023072801095373600_bib45) 2003; 13 Ivaska (2023072801095373600_bib23) 2011; 27 Saldanha (2023072801095373600_bib41) 2004; 20 Jia (2023072801095373600_bib24) 2019; 75 Mellacheruvu (2023072801095373600_bib33) 2013; 10 |
| References_xml | – volume: 47 start-page: D442 year: 2019 ident: 2023072801095373600_bib35 article-title: The PRIDE database and related tools and resources in 2019: improving support for quantification data publication-title: Nucleic Acids Res. doi: 10.1093/nar/gky1106 contributor: fullname: Perez-Riverol – volume: 39 start-page: 93 year: 2016 ident: 2023072801095373600_bib5 article-title: Adhesion protein networks reveal functions proximal and distal to cell-matrix contacts publication-title: Curr. Opin. Cell Biol. doi: 10.1016/j.ceb.2016.02.013 contributor: fullname: Byron – volume: 20 start-page: 1453 year: 2004 ident: 2023072801095373600_bib13 article-title: Open source clustering software publication-title: Bioinformatics. doi: 10.1093/bioinformatics/bth078 contributor: fullname: de Hoon – volume: 3 start-page: 145 year: 2014 ident: 2023072801095373600_bib34 article-title: CyKEGGParser: tailoring KEGG pathways to fit into systems biology analysis workflows publication-title: F1000 Res. doi: 10.12688/f1000research.4410.2 contributor: fullname: Nersisyan – volume: 219 year: 2020 ident: 2023072801095373600_bib10 article-title: Topological features of integrin adhesion complexes revealed by multiplexed proximity biotinylation publication-title: J. Cell Biol. doi: 10.1083/jcb.202003038 contributor: fullname: Chastney – volume: 757 start-page: 297 year: 2012 ident: 2023072801095373600_bib30 article-title: Isolation of focal adhesion proteins for biochemical and proteomic analysis publication-title: Methods Mol. Biol. doi: 10.1007/978-1-61779-166-6_19 contributor: fullname: Kuo – volume: 163 start-page: 409 year: 2003 ident: 2023072801095373600_bib17 article-title: Talin1 is critical for force-dependent reinforcement of initial integrin-cytoskeleton bonds but not tyrosine kinase activation publication-title: J. Cell Biol. doi: 10.1083/jcb.200302001 contributor: fullname: Giannone – volume: 11 start-page: 395 year: 2010 ident: 2023072801095373600_bib36 article-title: MZmine 2: modular framework for processing, visualizing, and analyzing mass spectrometry-based molecular profile data publication-title: BMC Bioinformatics. doi: 10.1186/1471-2105-11-395 contributor: fullname: Pluskal – volume: 325 start-page: 1240 year: 2009 ident: 2023072801095373600_bib1 article-title: Poly(ADP-ribose)-dependent regulation of DNA repair by the chromatin remodeling enzyme ALC1 publication-title: Science. doi: 10.1126/science.1177321 contributor: fullname: Ahel – volume: 9 start-page: rs4 year: 2016 ident: 2023072801095373600_bib15 article-title: Proximity biotinylation provides insight into the molecular composition of focal adhesions at the nanometer scale publication-title: Sci. Signal. doi: 10.1126/scisignal.aaf3572 contributor: fullname: Dong – volume: 10 start-page: 730 year: 2013 ident: 2023072801095373600_bib33 article-title: The CRAPome: a contaminant repository for affinity purification-mass spectrometry data publication-title: Nat. Methods. doi: 10.1038/nmeth.2557 contributor: fullname: Mellacheruvu – volume: 429 start-page: 515 year: 2017 ident: 2023072801095373600_bib50 article-title: The Interconnections between Autophagy and Integrin-Mediated Cell Adhesion publication-title: J. Mol. Biol. doi: 10.1016/j.jmb.2016.11.027 contributor: fullname: Vlahakis – volume: 27 start-page: 1188 year: 2016 ident: 2023072801095373600_bib28 article-title: An improved smaller biotin ligase for BioID proximity labeling publication-title: Mol. Biol. Cell. doi: 10.1091/mbc.E15-12-0844 contributor: fullname: Kim – volume: 116 start-page: 4605 year: 2003 ident: 2023072801095373600_bib58 article-title: Early molecular events in the assembly of matrix adhesions at the leading edge of migrating cells publication-title: J. Cell Sci. doi: 10.1242/jcs.00792 contributor: fullname: Zaidel-Bar – volume: 33 year: 2005 ident: 2023072801095373600_bib59 article-title: WebGestalt: an integrated system for exploring gene sets in various biological contexts publication-title: Nucleic Acids Res. doi: 10.1093/nar/gki475 contributor: fullname: Zhang – volume: 12 start-page: 1685 year: 2016 ident: 2023072801095373600_bib56 article-title: Autophagy promotes cell motility by driving focal adhesion turnover publication-title: Autophagy. doi: 10.1080/15548627.2016.1212791 contributor: fullname: Xu – volume: 27 start-page: 1739 year: 2011 ident: 2023072801095373600_bib31 article-title: Molecular signatures database (MSigDB) 3.0 publication-title: Bioinformatics. doi: 10.1093/bioinformatics/btr260 contributor: fullname: Liberzon – volume: 21 start-page: 101 year: 2020 ident: 2023072801095373600_bib3 article-title: Lysosomes as dynamic regulators of cell and organismal homeostasis publication-title: Nat. Rev. Mol. Cell Biol. doi: 10.1038/s41580-019-0185-4 contributor: fullname: Ballabio – volume: 17 start-page: 1577 year: 2015 ident: 2023072801095373600_bib22 article-title: Definition of a consensus integrin adhesome and its dynamics during adhesion complex assembly and disassembly publication-title: Nat. Cell Biol. doi: 10.1038/ncb3257 contributor: fullname: Horton – volume: 6 start-page: 1644 year: 2017 ident: 2023072801095373600_bib25 article-title: Autophagy promotes degradation of internalized collagen and regulates distribution of focal adhesions to suppress cell adhesion publication-title: Biol. Open. doi: 10.1242/bio.027458 contributor: fullname: Kawano – volume: 15 start-page: 273 year: 2014 ident: 2023072801095373600_bib53 article-title: The integrin adhesome: from genes and proteins to human disease publication-title: Nat. Rev. Mol. Cell Biol. doi: 10.1038/nrm3769 contributor: fullname: Winograd-Katz – volume: 216 start-page: 1949 year: 2017 ident: 2023072801095373600_bib8 article-title: Persistent mTORC1 signaling in cell senescence results from defects in amino acid and growth factor sensing publication-title: J. Cell Biol. doi: 10.1083/jcb.201610113 contributor: fullname: Carroll – volume: 26 start-page: 1367 year: 2008 ident: 2023072801095373600_bib11 article-title: MaxQuant enables high peptide identification rates, individualized p.p.b.-range mass accuracies and proteome-wide protein quantification publication-title: Nat. Biotechnol. doi: 10.1038/nbt.1511 contributor: fullname: Cox – volume: 111 start-page: E2453 year: 2014 ident: 2023072801095373600_bib27 article-title: Probing nuclear pore complex architecture with proximity-dependent biotinylation publication-title: Proc. Natl. Acad. Sci. USA. doi: 10.1073/pnas.1406459111 contributor: fullname: Kim – volume: 10 start-page: 398 year: 2015 ident: 2023072801095373600_bib39 article-title: Ligand-Occupied Integrin Internalization Links Nutrient Signaling to Invasive Migration publication-title: Cell Rep. doi: 10.1016/j.celrep.2014.12.037 contributor: fullname: Rainero – volume: 13 start-page: 731 year: 2016 ident: 2023072801095373600_bib49 article-title: The Perseus computational platform for comprehensive analysis of (prote)omics data publication-title: Nat. Methods. doi: 10.1038/nmeth.3901 contributor: fullname: Tyanova – volume: 20 start-page: 3246 year: 2004 ident: 2023072801095373600_bib41 article-title: Java Treeview--extensible visualization of microarray data publication-title: Bioinformatics. doi: 10.1093/bioinformatics/bth349 contributor: fullname: Saldanha – volume: 10 start-page: 1767 year: 2015 ident: 2023072801095373600_bib60 article-title: Dynamic Visualization of mTORC1 Activity in Living Cells publication-title: Cell Rep. doi: 10.1016/j.celrep.2015.02.031 contributor: fullname: Zhou – volume: 285 start-page: 1948 year: 2018 ident: 2023072801095373600_bib6 article-title: Nutrient sensing, growth and senescence publication-title: FEBS J. doi: 10.1111/febs.14400 contributor: fullname: Carroll – volume: 168 start-page: 960 year: 2017 ident: 2023072801095373600_bib43 article-title: mTOR Signaling in Growth, Metabolism, and Disease publication-title: Cell. doi: 10.1016/j.cell.2017.02.004 contributor: fullname: Saxton – volume: 1764 start-page: 193 year: 2018 ident: 2023072801095373600_bib4 article-title: Proteomic Profiling of Integrin Adhesion Complex Assembly publication-title: Methods Mol. Biol. doi: 10.1007/978-1-4939-7759-8_13 contributor: fullname: Byron – volume: 15 start-page: 915 year: 2019 ident: 2023072801095373600_bib20 article-title: A spatially and functionally distinct pool of TORC1 defines signaling endosomes in yeast publication-title: Autophagy. doi: 10.1080/15548627.2019.1580107 contributor: fullname: Hatakeyama – volume: 102 start-page: 15545 year: 2005 ident: 2023072801095373600_bib47 article-title: Gene set enrichment analysis: a knowledge-based approach for interpreting genome-wide expression profiles publication-title: Proc. Natl. Acad. Sci. USA. doi: 10.1073/pnas.0506580102 contributor: fullname: Subramanian – volume: 26 start-page: 301 year: 2017 ident: 2023072801095373600_bib54 article-title: The Dawn of the Age of Amino Acid Sensors for the mTORC1 Pathway publication-title: Cell Metab. doi: 10.1016/j.cmet.2017.07.001 contributor: fullname: Wolfson – volume: 194 start-page: 815 year: 2011 ident: 2023072801095373600_bib32 article-title: Review series: TOR kinase complexes and cell migration publication-title: J. Cell Biol. doi: 10.1083/jcb.201102090 contributor: fullname: Liu – volume: 30 start-page: 918 year: 2012 ident: 2023072801095373600_bib9 article-title: A cross-platform toolkit for mass spectrometry and proteomics publication-title: Nat. Biotechnol. doi: 10.1038/nbt.2377 contributor: fullname: Chambers – volume: 19 start-page: 818 year: 2018 ident: 2023072801095373600_bib38 article-title: mTORC1 and Nutrient Homeostasis: The Central Role of the Lysosome publication-title: Int. J. Mol. Sci. doi: 10.3390/ijms19030818 contributor: fullname: Rabanal-Ruiz – volume: 195 start-page: 499 year: 2011 ident: 2023072801095373600_bib51 article-title: The C terminus of talin links integrins to cell cycle progression publication-title: J. Cell Biol. doi: 10.1083/jcb.201104128 contributor: fullname: Wang – volume: 38 year: 2010 ident: 2023072801095373600_bib52 article-title: The GeneMANIA prediction server: biological network integration for gene prioritization and predicting gene function publication-title: Nucleic Acids Res. doi: 10.1093/nar/gkq537 contributor: fullname: Warde-Farley – volume: 107 start-page: 709 year: 1994 ident: 2023072801095373600_bib55 article-title: Characterisation of the paxillin-binding site and the C-terminal focal adhesion targeting sequence in vinculin publication-title: J. Cell Sci. doi: 10.1242/jcs.107.2.709 contributor: fullname: Wood – volume: 5 year: 2016 ident: 2023072801095373600_bib7 article-title: Control of TSC2-Rheb signaling axis by arginine regulates mTORC1 activity publication-title: eLife. doi: 10.7554/eLife.11058 contributor: fullname: Carroll – volume: 17 start-page: 1950 year: 2016 ident: 2023072801095373600_bib19 article-title: BORC Functions Upstream of Kinesins 1 and 3 to Coordinate Regional Movement of Lysosomes along Different Microtubule Tracks publication-title: Cell Rep. doi: 10.1016/j.celrep.2016.10.062 contributor: fullname: Guardia – volume: 75 start-page: 26 year: 2019 ident: 2023072801095373600_bib24 article-title: Lysosome Positioning Influences mTORC2 and AKT Signaling publication-title: Mol. Cell. doi: 10.1016/j.molcel.2019.05.009 contributor: fullname: Jia – volume: 13 start-page: 453 year: 2011 ident: 2023072801095373600_bib29 article-title: Lysosomal positioning coordinates cellular nutrient responses publication-title: Nat. Cell Biol. doi: 10.1038/ncb2204 contributor: fullname: Korolchuk – volume: 212 start-page: 577 year: 2016 ident: 2023072801095373600_bib26 article-title: NBR1 enables autophagy-dependent focal adhesion turnover publication-title: J. Cell Biol. doi: 10.1083/jcb.201503075 contributor: fullname: Kenific – volume: 2 start-page: 1896 year: 2007 ident: 2023072801095373600_bib40 article-title: Protocol for micro-purification, enrichment, pre-fractionation and storage of peptides for proteomics using StageTips publication-title: Nat. Protoc. doi: 10.1038/nprot.2007.261 contributor: fullname: Rappsilber – volume: 10 start-page: 1794 year: 2011 ident: 2023072801095373600_bib12 article-title: Andromeda: a peptide search engine integrated into the MaxQuant environment publication-title: J. Proteome Res. doi: 10.1021/pr101065j contributor: fullname: Cox – volume: 141 start-page: 290 year: 2010 ident: 2023072801095373600_bib42 article-title: Ragulator-Rag complex targets mTORC1 to the lysosomal surface and is necessary for its activation by amino acids publication-title: Cell. doi: 10.1016/j.cell.2010.02.024 contributor: fullname: Sancak – volume: 5 year: 2016 ident: 2023072801095373600_bib48 article-title: Kindlin-2 cooperates with talin to activate integrins and induces cell spreading by directly binding paxillin publication-title: eLife. doi: 10.7554/eLife.10130 contributor: fullname: Theodosiou – volume: 4 start-page: E75 year: 2002 ident: 2023072801095373600_bib57 article-title: Integrin regulation of growth factor receptors publication-title: Nat. Cell Biol. doi: 10.1038/ncb0402-e75 contributor: fullname: Yamada – volume: 32 start-page: 4932 year: 2013 ident: 2023072801095373600_bib2 article-title: Regulation of autophagy during ECM detachment is linked to a selective inhibition of mTORC1 by PERK publication-title: Oncogene. doi: 10.1038/onc.2012.512 contributor: fullname: Avivar-Valderas – volume: 17 start-page: 823 year: 2016 ident: 2023072801095373600_bib46 article-title: Folliculin directs the formation of a Rab34-RILP complex to control the nutrient-dependent dynamic distribution of lysosomes publication-title: EMBO Rep. doi: 10.15252/embr.201541382 contributor: fullname: Starling – volume: 27 start-page: 291 year: 2011 ident: 2023072801095373600_bib23 article-title: Cooperation between integrins and growth factor receptors in signaling and endocytosis publication-title: Annu. Rev. Cell Dev. Biol. doi: 10.1146/annurev-cellbio-092910-154017 contributor: fullname: Ivaska – volume: 129 start-page: 4329 year: 2016 ident: 2023072801095373600_bib37 article-title: Mechanisms and functions of lysosome positioning publication-title: J. Cell Sci. doi: 10.1242/jcs.196287 contributor: fullname: Pu – volume: 205 start-page: 525 year: 2014 ident: 2023072801095373600_bib44 article-title: The late endosomal p14-MP1 (LAMTOR2/3) complex regulates focal adhesion dynamics during cell migration publication-title: J. Cell Biol. doi: 10.1083/jcb.201310043 contributor: fullname: Schiefermeier – volume: 13 start-page: 2498 year: 2003 ident: 2023072801095373600_bib45 article-title: Cytoscape: a software environment for integrated models of biomolecular interaction networks publication-title: Genome Res. doi: 10.1101/gr.1239303 contributor: fullname: Shannon – volume: 216 start-page: 4217 year: 2017 ident: 2023072801095373600_bib21 article-title: PtdIns3P controls mTORC1 signaling through lysosomal positioning publication-title: J. Cell Biol. doi: 10.1083/jcb.201611073 contributor: fullname: Hong – volume: 1853 start-page: 2183 issue: 10 year: 2015 ident: 2023072801095373600_bib16 article-title: Modulation of focal adhesion constituents and their down-stream events by EGF: On the cross-talk of integrins and growth factor receptors publication-title: Biochim. Biophys. Acta. doi: 10.1016/j.bbamcr.2015.06.004 contributor: fullname: Eberwein |
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| Title | mTORC1 activity is supported by spatial association with focal adhesions |
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