Peripheral Type I Interferon Receptor Correlated with Oxidative Stress in Chronic Hepatitis B Virus Infection

• Published in: Journal of interferon & cytokine research Vol. 33; no. 8; pp. 405 - 414
• Main Authors: Zhao, Jing, Fan, Yu-Chen, Sun, Feng-Kai, Zhao, Ze-Hua, Wang, Li-Yuan, Hu, Lei-Hua, Yin, Yan-Ping, Li, Tao, Gao, Shuai, Wang, Kai
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.08.2013
• Subjects: Adult; Enzyme-Linked Immunosorbent Assay; Female; Flow Cytometry; Gene Expression; Glutathione - blood; Glutathione Peroxidase - blood; Glutathione Transferase - blood; Hepatitis B, Chronic - blood; Hepatitis B, Chronic - genetics; Hepatitis B, Chronic - metabolism; Humans; Liver Cirrhosis - blood; Liver Cirrhosis - genetics; Liver Cirrhosis - metabolism; Lymphocytes - metabolism; Male; Malondialdehyde - blood; Middle Aged; Monocytes - metabolism; Oxidative Stress; Receptor, Interferon alpha-beta - blood; Receptor, Interferon alpha-beta - genetics; Receptor, Interferon alpha-beta - metabolism; Research Reports; Reverse Transcriptase Polymerase Chain Reaction; Xanthine Oxidase - blood
• DOI: 10.1089/jir.2012.0153
• ISSN: 1079-9907; 1557-7465

Type I interferon receptor (IFNAR) has been involved in the progression of chronic hepatitis B (CHB). Oxidative stress is also associated with hepatitis B virus (HBV) infection and might contribute to the structure and function of protein synthesis including the IFNAR family. This study was aimed to determine the possible associations between oxidative stress and peripheral IFNAR expression in chronic HBV infection. Fifty-four CHB patients and 31 liver cirrhosis (LC) patients were consecutively collected, as well as 11 healthy subjects as controls. Expression levels of IFNAR1 and IFNAR2 in peripheral blood lymphocytes and monocytes were measured by flow cytometry. IFNAR1 and IFNAR2c mRNA were detected by real-time reverse transcription-polymerase chain reaction. Levels of plasma-soluble IFNAR and oxidative stress parameters, including xanthine oxidase (XOD), malondialdehyde (MDA), glutathione (GSH), glutathione S-transferase (GST), and glutathione peroxidase (GSH-Px) were detected by enzyme linked immunosorbent assay (ELISA). The frequencies of IFNAR1 and IFNAR2 in lymphocytes and monocytes were significantly increased in CHB and LC patients than in healthy controls. Expression levels of IFNAR1 and IFNAR2c mRNA and plasma-soluble IFNAR level in CHB and LC patients were upregulated compared with healthy controls. Mean fluorescence intensity (MFI) of IFNAR2 in monocytes of CHB patients was higher than that in LC patients. Levels of plasma XOD, MDA, and GST were significantly increased in CHB and LC patients compared with healthy controls. Meanwhile, GSH and GSH-Px in CHB and LC patients were decreased than that in healthy controls. Furthermore, plasma MDA, GSH, and GST levels in CHB patients were higher than that in LC patients. In CHB patients, plasma GST level was negatively correlated with MFI of IFNAR2 in lymphocytes. Our results suggested that oxidative stress play an important role in the regulation of IFNAR in chronic HBV infection.