High-Throughput and Accurate Determination of Transgene Copy Number and Zygosity in Transgenic Maize: From DNA Extraction to Data Analysis

• Published in: International journal of molecular sciences Vol. 22; no. 22; p. 12487
• Main Authors: Liu, Fang, Cheng, Jinkui, Liu, Xuhua, Wang, Xi-Qing
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 19.11.2021; MDPI
• Subjects: Automation; Biotechnology; Corn; Cultivars; Data analysis; DNA Copy Number Variations - genetics; DNA Primers; Efficiency; Gene Dosage - genetics; Genes; Genomes; Plant Breeding; Plants, Genetically Modified - genetics; Transgenes - genetics; Transgenic plants; Zea mays - genetics; Southern blot; digital PCR; zygosity; maize endogenous reference gene; TaqMan assay; transgene copy number; single-nucleotide polymorphism; high throughput

It is vital to develop high-throughput methods to determine transgene copy numbers initially and zygosity during subsequent breeding. In this study, the target sequence of the previously reported endogenous reference gene hmg was analyzed using 633 maize inbred lines, and two SNPs were observed. The...