Transcription rate strongly affects splicing fidelity and cotranscriptionality in budding yeast

• Published in: Genome research Vol. 28; no. 2; pp. 203 - 213
• Main Authors: Aslanzadeh, Vahid, Huang, Yuanhua, Sanguinetti, Guido, Beggs, Jean D.
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.02.2018
• Subjects: Alternative Splicing; DNA-directed RNA polymerase; Efficiency; Elongation; Exons - genetics; Fidelity; Gene expression; Introns - genetics; Mammalian cells; Protein structure; Proteins; RNA polymerase; RNA Polymerase II - genetics; RNA Splice Sites - genetics; RNA Splicing - genetics; Saccharomycetales - genetics; Secondary structure; Transcription; Transcription Elongation, Genetic; Transcription, Genetic; Yeast

The functional consequences of alternative splicing on altering the transcription rate have been the subject of intensive study in mammalian cells but less is known about effects of splicing on changing the transcription rate in yeast. We present several lines of evidence showing that slow RNA polym...