Fat Body Organ Culture System in Aedes Aegypti, a Vector of Zika Virus

• Published in: Journal of visualized experiments no. 126
• Main Authors: Chung, Hae-Na, Rodriguez, Stacy D., Carpenter, Victoria K., Vulcan, Julia, Bailey, C. Donovan, Nageswara-Rao, Madhugiri, Li, Yiyi, Attardo, Geoffrey M., Hansen, Immo A.
• Format: Journal Article
• Language: English
• Published: United States MyJove Corporation 19.08.2017
• Subjects: abdomen; Aedes - genetics; Aedes - metabolism; Aedes - virology; Aedes aegypti; amino acids; Animals; arboviruses; calcium; culture media; dengue; disease vectors; egg production; Egg Proteins - genetics; excision; fat body; Fat Body - metabolism; Fat Body - virology; Gene Expression; gene expression regulation; Infectious Diseases; Insect Vectors - genetics; Insect Vectors - metabolism; Insect Vectors - virology; insects; integument; liver; metabolism; metabolomics; organ culture; Organ Culture Techniques - methods; proteomics; quantitative polymerase chain reaction; RNA; sequence analysis; vertebrates; Western blotting; yolk proteins; Zika Virus

The insect fat body plays a central role in insect metabolism and nutrient storage, mirroring functions of the liver and fat tissue in vertebrates. Insect fat body tissue is usually distributed throughout the insect body. However, it is often concentrated in the abdomen and attached to the abdominal...