Chromosome assembly of large and complex genomes using multiple references

• Published in: Genome research Vol. 28; no. 11; pp. 1720 - 1732
• Main Authors: Kolmogorov, Mikhail, Armstrong, Joel, Raney, Brian J., Streeter, Ian, Dunn, Matthew, Yang, Fengtang, Odom, Duncan, Flicek, Paul, Keane, Thomas M., Thybert, David, Paten, Benedict, Pham, Son
• Format: Journal Article
• Language: English
• Published: United States Cold Spring Harbor Laboratory Press 01.11.2018
• Subjects: Animals; Bioinformatics; Chromosomes; Contig Mapping - methods; Contig Mapping - standards; Gene mapping; Genomes; Karyotypes; Method; Mice; Reference Standards; Whole Genome Sequencing - methods; Whole Genome Sequencing - standards

Despite the rapid development of sequencing technologies, the assembly of mammalian-scale genomes into complete chromosomes remains one of the most challenging problems in bioinformatics. To help address this difficulty, we developed Ragout 2, a reference-assisted assembly tool that works for large...