Simultaneous Determination of Quercetin, Rutin, Naringin, and Naringenin in Different Fruits by Capillary Zone Electrophoresis

A fast and reliable capillary zone electrophoretic (CZE) method has been developed for the simultaneous determination of four fruit flavonoids using photodiode array (PDA) detector. The effects of CE parameters including concentration and pH of the running buffer, voltage, and injection time were op...

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Published inFood analytical methods Vol. 10; no. 1; pp. 83 - 91
Main Authors Memon, Almas F., Solangi, Amber R., Memon, Saima Q., Mallah, Arfana, Memon, Najma, Memon, Ayaz A.
Format Journal Article
LanguageEnglish
Published New York Springer US 01.01.2017
Springer Nature B.V
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ISSN1936-9751
1936-976X
DOI10.1007/s12161-016-0552-0

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Abstract A fast and reliable capillary zone electrophoretic (CZE) method has been developed for the simultaneous determination of four fruit flavonoids using photodiode array (PDA) detector. The effects of CE parameters including concentration and pH of the running buffer, voltage, and injection time were optimized. Under the optimized conditions, all flavonoids were well determined in a 10 mM borate buffer of pH 8.5 within 10 min at an applied voltage of 25 kV. Naringin, naringenin, and quercetin were found to have linear response in the range of 3.12–200 μg/mL whereas rutin’s response was linear from 6.25 to 200 μg/mL. LOD was found to be 0.406, 0.314, 0.582, and 0.333 μg/mL and LOQ 1.355, 1.046, 1.941, and 1.11, respectively. Relative standard deviations (RSD) were found to be less than 3 % for both migration time and peak height which shows long-term stability and good reproducibility of the developed method. The method was successfully applied for the simultaneous determination of flavonoids from various fruit juice samples.
AbstractList A fast and reliable capillary zone electrophoretic (CZE) method has been developed for the simultaneous determination of four fruit flavonoids using photodiode array (PDA) detector. The effects of CE parameters including concentration and pH of the running buffer, voltage, and injection time were optimized. Under the optimized conditions, all flavonoids were well determined in a 10 mM borate buffer of pH 8.5 within 10 min at an applied voltage of 25 kV. Naringin, naringenin, and quercetin were found to have linear response in the range of 3.12–200 μg/mL whereas rutin’s response was linear from 6.25 to 200 μg/mL. LOD was found to be 0.406, 0.314, 0.582, and 0.333 μg/mL and LOQ 1.355, 1.046, 1.941, and 1.11, respectively. Relative standard deviations (RSD) were found to be less than 3 % for both migration time and peak height which shows long-term stability and good reproducibility of the developed method. The method was successfully applied for the simultaneous determination of flavonoids from various fruit juice samples.
A fast and reliable capillary zone electrophoretic (CZE) method has been developed for the simultaneous determination of four fruit flavonoids using photodiode array (PDA) detector. The effects of CE parameters including concentration and pH of the running buffer, voltage, and injection time were optimized. Under the optimized conditions, all flavonoids were well determined in a 10 mM borate buffer of pH 8.5 within 10 min at an applied voltage of 25 kV. Naringin, naringenin, and quercetin were found to have linear response in the range of 3.12–200 μg/mL whereas rutin’s response was linear from 6.25 to 200 μg/mL. LOD was found to be 0.406, 0.314, 0.582, and 0.333 μg/mL and LOQ 1.355, 1.046, 1.941, and 1.11, respectively. Relative standard deviations (RSD) were found to be less than 3 % for both migration time and peak height which shows long-term stability and good reproducibility of the developed method. The method was successfully applied for the simultaneous determination of flavonoids from various fruit juice samples.
Author Memon, Najma
Solangi, Amber R.
Mallah, Arfana
Memon, Ayaz A.
Memon, Almas F.
Memon, Saima Q.
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Keywords CE
Flavonoids
Naringin
Naringenin
Quercetin and rutin in fruits
Language English
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Snippet A fast and reliable capillary zone electrophoretic (CZE) method has been developed for the simultaneous determination of four fruit flavonoids using photodiode...
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SubjectTerms Analytical Chemistry
borates
Buffers
capillary zone electrophoresis
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Electric potential
Electrophoresis
Flavonoids
Food safety
Food Science
fruit juices
fruits
Microbiology
naringenin
naringin
Photodiodes
quercetin
rutin
statistical analysis
Voltage
Title Simultaneous Determination of Quercetin, Rutin, Naringin, and Naringenin in Different Fruits by Capillary Zone Electrophoresis
URI https://link.springer.com/article/10.1007/s12161-016-0552-0
https://www.proquest.com/docview/1880806161
https://www.proquest.com/docview/2000511430
Volume 10
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