A novel high-throughput automated chip-based nanoelectrospray tandem mass spectrometric method for PAMPA sample analysis

• Published in: Journal of pharmaceutical and biomedical analysis Vol. 39; no. 1; pp. 8 - 16
• Main Authors: Balimane, Praveen V., Pace, Ellen, Chong, Saeho, Zhu, Mingshe, Jemal, Mohammed, Pelt, Colleen K. Van
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.09.2005; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Automation; Biological and medical sciences; Chromatography, High Pressure Liquid; Drug discovery; Fundamental and applied biological sciences. Psychology; General pharmacology; High-throughput screening; HPLC; LC/MS; Medical sciences; Microchip Analytical Procedures; Nanoelectrospray analysis; Nanotechnology; PAMPA permeability; Permeability; Permeability-screening; Pharmacology. Drug treatments; Spectrometry, Mass, Electrospray Ionization - instrumentation; Spectrophotometry, Ultraviolet; P-gp; PAMPA permeability; ADME; DMSO; S.D; High-throughput screening; PAMPA; LC/MS; NCE; Pc; IAM; MDCK; Permeability-screening; Drug discovery; HPLC; Nanoelectrospray analysis; High throughput screening; Drug; HPLC chromatography; Liquid chromatography; Permeability; Research and development; Permeability-screening: Nanoelectrospray analysis; Mass spectrometry MS/MS; Mass spectrometry

Parallel artificial membrane permeability assay (PAMPA) has recently gained popularity as a novel, high-throughput assay capable of rapidly screening compounds for their permeability characteristics in early drug discovery. The analytical techniques typically used for PAMPA sample analysis are HPLC-...