A new tool for studying the molecular architecture of the fungal cell wall: one-step purification of recombinant Trichoderma β-(1-6)-glucanase expressed in Pichia pastoris - Bom, I.J.; Dielbandhoesing, S.K.; Harvey, K.N.; Oomes, S.J.C.M.; Klis, F.M.; Brul, S. (Biochimica et biophysica acta, 1998)
Keywords: beta-glucanase; biosynthesis; Cell Membrane; Cell Membrane - chemistry; cell wall components; chemistry; Enzyme purification; Enzyme Stability; enzymology; Fungal cell wall; genetics; Glycoside Hydrolases; Glycoside Hydrolases - biosynthesis; Glycoside Hydrolases - chemistry; Glycoside Hydrolases - isolation & purification; Heterogeneous protein expression; Hydrogen-Ion Concentration; isolation & purification; Mannoprotein; Pichia; Pichia - enzymology; Pichia - genetics; Recombinant Proteins; Recombinant Proteins - isolation & purification; Trichoderma; Trichoderma - enzymology; Trichoderma - genetics; β-(1-6)-Glucanase; Enzyme purification; β-(1-6)-Glucanase; Mannoprotein; Heterogeneous protein expression; Fungal cell wall