Clostridium botulinum Spores Found in Honey from Small Apiaries in Poland

• Published in: Journal of Apicultural Science Vol. 60; no. 2; pp. 89 - 100
• Main Authors: Wojtacka, Joanna, Wysok, Beata, Lipiński, Zbigniew, Gomółka-Pawlicka, Małgorzata, Rybak-Chmielewska, Helena, Wiszniewska-Łaszczych, Agnieszka
• Format: Journal Article
• Language: English
• Published: Pulawy De Gruyter Open 01.12.2016; De Gruyter Poland; Sciendo
• Subjects: Agar; apiaries; Broths; Centrifugation; Clostridium botulinum; Clostridium botulinum E; Contamination; cooked foods; Coordinate measuring machines; culture media; Dilution; Glucose; Honey; Meat; Multiplexing; Neurotoxins; PCR; Peptones; Poland; polymerase chain reaction; Quantitative analysis; Sample contamination; Sampling; serotypes; Specimen preparation; Spores; Toxins; Yeast; yeasts; Poland

A total of 102 honey samples collected from small apiaries (≤ 20 hives) in Poland were analysed for the presence of spores. The samples were prepared using the dilution centrifugation method and cultured in parallel in cooked meat medium (CMM) and tripticase peptone glucose yeast (TPGY) enrichment b...