Influenza hemagglutinin-specific IgA Fc-effector functionality is restricted to stalk epitopes

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 118; no. 8; pp. 1 - 11
• Main Authors: Freyn, Alec W., Han, Julianna, Guthmiller, Jenna J., Bailey, Mark J., Neu, Karlynn, Turner, Hannah L., Rosado, Victoria C., Chromikova, Veronika, Huang, Min, Strohmeier, Shirin, Liu, Sean T. H., Simon, Viviana, Krammer, Florian, Ward, Andrew B., Palese, Peter, Wilson, Patrick C., Nachbagauer, Raffael
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 23.02.2021
• Subjects: Adult; Animals; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - metabolism; Antibody Affinity; Binding Sites, Antibody; Biological Sciences; Chick Embryo; Cryoelectron Microscopy; Epitopes - immunology; Female; Hemagglutinin Glycoproteins, Influenza Virus - genetics; Hemagglutinin Glycoproteins, Influenza Virus - immunology; Hemagglutinin Glycoproteins, Influenza Virus - metabolism; Humans; Immunoglobulin A - immunology; Immunoglobulin Fc Fragments - immunology; Influenza A Virus, H1N1 Subtype - immunology; Influenza Vaccines - immunology; Male; Neutrophils - immunology; Neutrophils - virology; influenza; IgA; Fc receptor

In this study, we utilized a panel of human immunoglobulin (Ig) IgA monoclonal antibodies isolated from the plasmablasts of eight donors after 2014/2015 influenza virus vaccination (Fluarix) to study the binding and functional specificities of this isotype. In this cohort, isolated IgA monoclonal an...