Reassemblable quasi-chip free-flow electrophoresis with simple heating dispersion for rapid micropreparation of trypsin in crude porcine pancreatin

• Published in: Electrophoresis Vol. 32; no. 22; pp. 3248 - 3256
• Main Authors: Geng, Jia-Zhen, Shao, Jing, Yang, Jing-Hua, Pang, Bo, Cao, Cheng-Xi, Fan, Liu-Yin
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.11.2011; WILEY‐VCH Verlag
• Subjects: Animals; electrophoresis; Electrophoresis, Microchip - instrumentation; Electrophoresis, Microchip - methods; Electrophoresis, Polyacrylamide Gel; Equipment Design; Free-flow electrophoresis; heat; Hydrogen-Ion Concentration; Micropreparation; Micropurification; pancreatin; Pancreatin - chemistry; Porcine pancreatin; proteins; solutes; Swine; Trypsin; Trypsin - analysis; Trypsin - chemistry; Trypsin - isolation & purification

An increasing number of small biosamples (e.g. proteins and enzymes) need micropreparation in lab. However, neither large‐scale free‐flow electrophoresis (LS‐FFE) nor chip FFE (C‐FFE) could fit the growing demands. Herein, a simple quasi‐chip FFE (QC‐FFE) was constructed. In contrast to C‐FFE, the f...