Surface antigen profiling of colorectal cancer using antibody microarrays with fluorescence multiplexing

• Published in: Journal of immunological methods Vol. 355; no. 1; pp. 40 - 51
• Main Authors: Zhou, Jerry, Belov, Larissa, Huang, Pauline Y., Shin, Joo-Shik, Solomon, Michael J., Chapuis, Pierre H., Bokey, Leslie, Chan, Charles, Clarke, Candice, Clarke, Stephen J., Christopherson, Richard I.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 15.04.2010; Elsevier
• Subjects: Adult; Aged; Aged, 80 and over; Antibodies, Monoclonal - chemistry; Antibodies, Neoplasm - chemistry; Antibody microarrays; Antigens, Neoplasm - metabolism; Biological and medical sciences; CD antigens; Cell Line, Tumor; Colorectal cancer; Colorectal Neoplasms - metabolism; Female; Fluorescence; Fluorescent Dyes - chemistry; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Leukocytes; Male; Middle Aged; Molecular immunology; Multiplexing; Protein Array Analysis - methods; Quantum Dots; Techniques; Multiplexing; CD; QDs; EpCAM; IHC; Colorectal cancer; Antibody microarrays; Fluorescence; Leukocytes; ACP; EGFR; CD antigens; BSA; TAMs; CRC; FCS; TILs; HBSS; CEA; MeV; Rectal disease; Antibody; Leukocyte; Malignant tumor; Immunological method; Colonic disease; Antigen; Digestive diseases; Intestinal disease; Cancer

A procedure is described for the disaggregation of colorectal cancers (CRC) and normal intestinal mucosal tissues to produce suspensions of viable single cells, which are then captured on customized antibody microarrays recognising 122 different surface antigens (DotScan™ CRC microarray). Cell bindi...