Receptors for the Chemoattractants C5a and IL-8 Are Clustered on the Surface of Human Neutrophils

• Published in: The journal of histochemistry and cytochemistry Vol. 45; no. 11; pp. 1461 - 1467
• Main Authors: Gray, Gary D, Hasslen, Sharon R, Ember, Julia A, Carney, David F, Herron, Michael J, Erlandsen, Stanley L, Nelson, Robert D
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA Histochemical Soc 01.11.1997; SAGE Publications
• Subjects: Animals; Antigens, CD - analysis; Antigens, CD - genetics; Female; Fibroblasts - chemistry; Humans; Male; Mice; Microscopy, Electron, Scanning; Microscopy, Immunoelectron; Neutrophil Activation - immunology; Neutrophils - chemistry; Neutrophils - ultrastructure; Receptor, Anaphylatoxin C5a; Receptors, Complement - analysis; Receptors, Complement - genetics; Receptors, Interleukin - analysis; Receptors, Interleukin-8A; Transfection; chemoattractant receptor; chemokine; IL-8 receptor; immunocytochemistry; field emission scanning electron microscopy (FESEM); mouse L-cells; complement; neutrophil; C5a receptor (CD88)

We have used high-resolution field emission scanning electron microscopy with backscatter electron imaging to detect immunogold-labeled C5a and interleukin-8 (IL-8) receptors on human blood neutrophils. The receptors were labeled with receptor-specific antibodies in combination with secondary antibo...