Development and validation of a method for simultaneous analysis of the boar taint compounds indole, skatole and androstenone in pig fat using liquid chromatography–multiple mass spectrometry

• Published in: Journal of Chromatography A Vol. 1174; no. 1; pp. 132 - 137
• Main Authors: Verheyden, K., Noppe, H., Aluwé, M., Millet, S., Vanden Bussche, J., De Brabander, H.F.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 07.12.2007; Elsevier
• Subjects: Analytical chemistry; Androstenes - analysis; Androstenone; Animals; Biological and medical sciences; Boar taint; body fat; Calibration; chemical analysis; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography, Liquid - methods; Exact sciences and technology; Fat matrix; food analysis; Food industries; Fundamental and applied biological sciences. Psychology; Indole; indoles; Indoles - analysis; lard; LC–MS n; Lipids - chemistry; liquid chromatography; mass spectrometry; Mass Spectrometry - methods; Meat and meat product industries; Other chromatographic methods; pork; Reproducibility of Results; Skatole; Skatole - analysis; swine; Swine - metabolism; Fat matrix; Skatole; Androstenone; Indole; LC–MS n; Boar taint; Steroid; Tandem mass spectrometry; Chemical analysis; Atmospheric pressure; LC-MSn; Solvent extraction; Electrospray; Off-flavor; Sample preparation; Ungulata; Quantitative analysis; Validation; Filtration; Coupled method; Chemical ionization; Pig; Vertebrata; Reversed phase chromatography; Mammalia; Simultaneous measurement; Fat; Artiodactyla; Indole derivatives

Boar taint in entire male pigs remains a problem for fresh pork production. Since castration of pigs will be prohibited in the future on animal welfare reasons, attempts are made to detect boar taint pre and post mortem. Post mortem techniques focus on simultaneous quantification of the three boar t...