An integrated high-performance liquid chromatography–mass spectrometry system for the activity-dependent analysis of matrix metalloproteases

• Published in: Journal of Chromatography A Vol. 1189; no. 1; pp. 417 - 425
• Main Authors: Freije, Robert, Klein, Theo, Ooms, Bert, Kauffman, Henk F., Bischoff, Rainer
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 02.05.2008; Amsterdam; New York: Elsevier; Elsevier
• Subjects: Activity-based analysis; Affinity chromatography; Automated online analysis system; Biological and medical sciences; Chromatography, Affinity; Chromatography, High Pressure Liquid - methods; HPLC; Humans; Investigative techniques, diagnostic techniques (general aspects); Mass spectrometry; Mass Spectrometry - methods; Matrix Metalloproteinase 12 - urine; Medical sciences; Metalloprotease; Metalloproteases - urine; Miscellaneous. Technology; MMPs; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Proteomics; Proteomics - methods; Targeted proteomics; Targeted proteomics; Affinity chromatography; Proteomics; MMPs; Metalloprotease; Activity-based analysis; Mass spectrometry; Automated online analysis system; HPLC; Capillary column; Glomerulonephritis; Solid phase extraction; Biological fluid; Chemical analysis; On line; Peptides; HPLC chromatography; Macrophage elastase; Metalloendopeptidases; Electrospray; Chemical enrichment; Affinity adsorbent; Sample preparation; Proteolysis; Clinical biology; Kidney disease; Human; Urine; Automation; Urinary system disease; Serine endopeptidases; Enzyme; Coupled method; Enzyme inhibitor; Patient; Protein; Peptidases; Bioreactor; Trypsin; Analysis method; Peptide fragment; Hydrolases; Immobilized enzyme

Matrix metalloproteases (MMPs) comprise a family of enzymes that play important roles in mediating angiogenesis, the remodelling of tissues and in cancer metastasis. Consequently, they are attractive targets for therapeutic intervention in chronic inflammation, cancer and neurological disorders. In...