Label-free and sensitive MiRNA detection based on turn-on fluorescence of DNA-templated silver nanoclusters coupled with duplex-specific nuclease-assisted signal amplification

• Published in: Mikrochimica acta (1966) Vol. 188; no. 10; p. 355
• Main Authors: Ma, Gui-min, Huo, Li-wei, Tong, Yin-xia, Wang, Yu-cong, Li, Cui-ping, Jia, Hong-xia
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.10.2021; Springer; Springer Nature B.V
• Subjects: Amplification; Analytical Chemistry; Biological properties; Biosensing Techniques - methods; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; DNA; DNA - chemistry; DNA Probes - chemistry; DNA Probes - genetics; DNA sequencing; Fluorescence; Gene sequencing; Humans; Limit of Detection; Metal Nanoparticles - chemistry; Microengineering; MicroRNA; MicroRNAs; MicroRNAs - analysis; Nanochemistry; Nanoclusters; Nanotechnology; Nuclease; Nucleic Acid Amplification Techniques - methods; Nucleic Acid Hybridization; Nucleotide sequencing; Original Paper; Selectivity; Silver - chemistry; Spectrometry, Fluorescence - methods; Duplex-specific nuclease-assisted signal amplification; Silver nanoclusters; MicroRNAs; DNA template; Fluorescence enhancement

A novel strategy for microRNAs (miRNAs) detection has been developed utilizing duplex-specific nuclease-assisted signal amplification (DSNSA) and guanine-rich DNA-enhanced fluorescence of DNA-templated silver nanoclusters (AgNCs). The combination between target miRNA, DSNSA, and AgNCs is achieved by...