Regulation of De Novo Adipocyte Differentiation Through Cross Talk Between Adipocytes and Preadipocytes

• Published in: Diabetes (New York, N.Y.) Vol. 64; no. 12; pp. 4075 - 4087
• Main Authors: Challa, Tenagne D., Straub, Leon G., Balaz, Miroslav, Kiehlmann, Elke, Donze, Olivier, Rudofsky, Gottfried, Ukropec, Jozef, Ukropcova, Barbara, Wolfrum, Christian
• Format: Journal Article
• Language: English
• Published: United States American Diabetes Association 01.12.2015
• Subjects: 3T3-L1 Cells; Adipocytes; Adipocytes - metabolism; Adipocytes - pathology; Adipocytes - secretion; Adipogenesis; Adult Stem Cells - metabolism; Adult Stem Cells - pathology; Adult Stem Cells - secretion; Animals; Body Mass Index; Cell Communication; Cell Size; Cells, Cultured; Coculture Techniques; Cohort Studies; Cytokines; Diabetes; Gene expression; Gene Expression Regulation, Developmental; HEK293 Cells; Humans; Male; Metabolism; Mice; Mice, Inbred C57BL; Obesity - metabolism; Obesity - pathology; Proteomics; Recombinant Proteins - chemistry; Recombinant Proteins - genetics; Recombinant Proteins - metabolism; Recombinant Proteins - secretion; RNA Interference; Specific Pathogen-Free Organisms; Subcutaneous Fat - metabolism; Subcutaneous Fat - pathology; Subcutaneous Fat - secretion; Subcutaneous Fat, Abdominal - metabolism; Subcutaneous Fat, Abdominal - pathology; Subcutaneous Fat, Abdominal - secretion; Tissue Culture Techniques; Weight

There are many known adipokines differentially secreted from the different adipose depots; however, their paracrine and autocrine effects on de novo adipocyte formation are not fully understood. By developing a coculture method of preadipocytes with primary subcutaneous and visceral adipocytes or ti...