Intracellular ROS scavenging and antioxidant regulation of WL15 from cysteine and glycine-rich protein 2 demonstrated in zebrafish in vivo model

• Published in: Developmental and comparative immunology Vol. 114; p. 103863
• Main Authors: Guru, Ajay, Lite, Christy, Freddy, Allen J., Issac, Praveen Kumar, Pasupuleti, Mukesh, Saraswathi, N.T., Arasu, Mariadhas Valan, Al-Dhabi, Naif Abdullah, Arshad, Aziz, Arockiaraj, Jesu
• Format: Journal Article
• Language: English
• Published: United States Elsevier Ltd 01.01.2021; Elsevier Science Ltd
• Subjects: 2,2-diphenyl-1-picrylhydrazyl; Amino acids; Animals; antioxidant activity; Antioxidant peptide; Antioxidants; Antioxidants - metabolism; Apoptosis; Bioactive compounds; Biocompatibility; Caspase-3; Catalase; Catalase - metabolism; Cells, Cultured; Channa striata; Cysteine; Cysteine and glycine-rich protein; cysteine synthase; Cytotoxicity; Danio rerio; Dermis - cytology; Developmental stages; developmental toxicity; dose response; Embryo, Nonmammalian; Embryonic Development; Embryos; Enzymes; Evaluation; Exposure; exposure duration; Fatty acids; Fertilization; fibroblasts; Fibroblasts - immunology; fluorescent antibody technique; Food; food animals; Food fish; Free Radical Scavengers - metabolism; Free radicals; freshwater; Freshwater fish; Gene expression; Glutathione; Glutathione transferase; Glycine; glycine (amino acid); humans; Hydrogen peroxide; Immunofluorescence; Intracellular; Larva; Larvae; Malondialdehyde; Muscle Proteins - genetics; Muscle Proteins - metabolism; Oxidative Stress; oxidative toxicity; Peptides; Peptides - genetics; Peptides - metabolism; Proteins; Reactive oxygen species; Reactive Oxygen Species - metabolism; Scavenging; superoxide anion; Superoxide dismutase; Superoxide Dismutase - metabolism; Toxicity; transcriptome; Zebrafish; Zebrafish - metabolism; Zebrafish Proteins - genetics; Zebrafish Proteins - metabolism; Cysteine and glycine-rich protein; Oxidative stress; Zebrafish; Antioxidant peptide; Apoptosis

Antioxidant peptides are naturally present in food, especially in fishes, and are considered to contain rich source of various bioactive compounds that are structurally heterogeneous. This study aims to identify and characterize the antioxidant property of the WL15 peptide, derived from Cysteine and...