A Protocol to Depict the Proteolytic Processes Using a Combination of Metal–Organic Materials (MOMs), Electron Paramagnetic Resonance (EPR), and Mass Spectrometry (MS)

• Published in: Bio-protocol Vol. 14; no. 1; p. e4909
• Main Authors: Li, Qiaobin, Lenertz, Mary, Armstrong, Zoe, MacRae, Austin, Feng, Li, Ugrinov, Angel, Yang, Zhongyu
• Format: Journal Article
• Language: English
• Published: United States Bio-Protocol 05.01.2024; Bio-protocol LLC
• Subjects: Biology; Methods; Metal–organic materials (MOMs); Site-directed spin labeling; Tandem MS; Protease immobilization; Electron paramagnetic resonance (EPR); Mass spectrometry (MS)

Proteolysis is a critical biochemical process yet a challenging field to study experimentally due to the self-degradation of a protease and the complex, dynamic degradation steps of a substrate. Mass spectrometry (MS) is the traditional way for proteolytic studies, yet it is challenging when time-re...