Gene Inactivation Study on gntK, a Putative C-methyltransferase Gene in Gentamicin Biosynthesis from Micromonospora echinospora

• Published in: Applied biological chemistry Vol. 55; no. 3; pp. 439 - 442
• Main Authors: Karki, Suman, Myongji University, Yongin, Republic of Korea, Kim, J.Y., Myongji University, Yongin, Republic of Korea, Park, S.H., Mokpo National University, Muan, Republic of Korea, Kwon, H.J., Myongji University, Yongin, Republic of Korea
• Format: Journal Article
• Language: English
• Published: Seoul The Korean Society for Applied Biological Chemistry 01.06.2012; Springer Nature B.V; 한국응용생명화학회
• Subjects: Adenosylmethionine; Applied Microbiology; Biological Techniques; Bioorganic Chemistry; Biosynthesis; Chemistry; Chemistry and Materials Science; Deactivation; Domains; GENE; GENES; Gentamicin; gentamicin biosynthesis; gntK; Inactivation; Isomers; Methylation; Methyltransferase; Micromonospora echinospora; S-Adenosylmethionine; Short Communication; Vitamin B12; 농학; gentamicin biosynthesis; methyltransferase; radical; adenosylmethionine domain
• ISSN: 1738-2203; 2468-0834; 2234-344X; 2468-0842
• DOI: 10.1007/s13765-012-2041-5

GntK harbors methyltransferase-related cobalaminbinding domain and radical S-adenosylmethionine domain. The gntK-inactivation mutant of Micromonospora echinospora accumulated higher levels of gentamicin Cla and lower levels of gentamicin C1 and C2 isomers compared to the wild-type strain. Based on these results, we propose that GntK is involved in Cmethylation on C-6' in gentamicin X2 but is dispensable in gentamicin biosynthesis.