Phosphorylation of LRRK2 by casein kinase 1α regulates trans-Golgi clustering via differential interaction with ARHGEF7

• Published in: Nature communications Vol. 5; no. 1; p. 5827
• Main Authors: Chia, Ruth, Haddock, Sara, Beilina, Alexandra, Rudenko, Iakov N., Mamais, Adamantios, Kaganovich, Alice, Li, Yan, Kumaran, Ravindran, Nalls, Michael A., Cookson, Mark R.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 15.12.2014
• Subjects: 13; 13/89; 14/63; 631/378/1689/1718; 631/45/275; 631/80/86; 82; 82/1; Animals; Benzamides - pharmacology; Casein Kinase Ialpha - antagonists & inhibitors; Casein Kinase Ialpha - genetics; Casein Kinase Ialpha - metabolism; Cerebral Cortex - cytology; Cerebral Cortex - drug effects; Cerebral Cortex - enzymology; Corpus Striatum - cytology; Corpus Striatum - enzymology; Gene Expression Regulation; HEK293 Cells; Humanities and Social Sciences; Humans; Imidazoles - pharmacology; Indoles - pharmacology; Leucine-Rich Repeat Serine-Threonine Protein Kinase-2; Membrane Glycoproteins - antagonists & inhibitors; Membrane Glycoproteins - genetics; Membrane Glycoproteins - metabolism; Mice; Mice, Inbred C57BL; Mice, Knockout; multidisciplinary; Neurons - cytology; Neurons - drug effects; Neurons - enzymology; Phloroglucinol - analogs & derivatives; Phloroglucinol - pharmacology; Phosphorylation; Primary Cell Culture; Protein Kinase Inhibitors - pharmacology; Protein Serine-Threonine Kinases - antagonists & inhibitors; Protein Serine-Threonine Kinases - genetics; Protein Serine-Threonine Kinases - metabolism; Rho Guanine Nucleotide Exchange Factors - antagonists & inhibitors; Rho Guanine Nucleotide Exchange Factors - genetics; Rho Guanine Nucleotide Exchange Factors - metabolism; RNA, Small Interfering - genetics; RNA, Small Interfering - metabolism; Science; Science (multidisciplinary); Signal Transduction; trans-Golgi Network - drug effects; trans-Golgi Network - metabolism

LRRK2, a gene relevant to Parkinson's disease, encodes a scaffolding protein with both GTPase and kinase activities. LRRK2 protein is itself phosphorylated and therefore is subject to regulation by cell signalling; however, the kinase(s) responsible for this event have not been definitively ide...