Polarized Expression of Monocarboxylate Transporters in Human Retinal Pigment Epithelium and ARPE-19 Cells

• Published in: Investigative ophthalmology & visual science Vol. 44; no. 4; pp. 1716 - 1721
• Main Authors: Philp, Nancy J, Wang, Dian, Yoon, Heeyong, Hjelmeland, Leonard M
• Format: Journal Article
• Language: English
• Published: Rockville, MD ARVO 01.04.2003; Association for Research in Vision and Ophtalmology
• Subjects: Animals; Antigens, CD; Antigens, Neoplasm; Antigens, Surface; Avian Proteins; Basigin; Biological and medical sciences; Blood Proteins; Blotting, Western; Carrier Proteins - genetics; Carrier Proteins - metabolism; Cell Line; Cell Membrane - metabolism; Eye and associated structures. Visual pathways and centers. Vision; Fluorescent Antibody Technique, Indirect; Fundamental and applied biological sciences. Psychology; Humans; Membrane Glycoproteins - metabolism; Membrane Transport Proteins; Monocarboxylic Acid Transporters - genetics; Monocarboxylic Acid Transporters - metabolism; Pigment Epithelium of Eye - metabolism; Rabbits; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - metabolism; Symporters - genetics; Symporters - metabolism; Vertebrates: nervous system and sense organs; Carboxylate polymer; Human; Cell culture; Pigment epithelium; Retina; Gene expression; Conveyor; Pigment cell

To evaluate the expression and subcellular distribution of proton-coupled monocarboxylate transporters (MCTs) in human RPE in vivo and determine whether ARPE-19 cells retain the ability to express and differentially polarize these transporters. Total RNA was prepared from human donor eyes and from A...