Enzymatic synthesis and characterization of chlorophyllide derivatives as possible internal standards for pigment chromatographic analysis

•Easy and fast enzymatic synthesis of chlorophyllides a and b esters.•Dunalliela salina provided both the enzyme chlorophyllase and chlorophyll substrates.•Chromatographic properties of esters of different alcohols and diols were studied.•Chlorophyllide a (8′-hydroxyoctyl) ester was selected and com...

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Published inAlgal research (Amsterdam) Vol. 46; p. 101688
Main Authors Gavalás-Olea, Antonio, Sanz, Noelia, Riobó, Pilar, Garrido, José Luis, Vaz, Belén
Format Journal Article
LanguageEnglish
Published Elsevier B.V 01.03.2020
Subjects
Chlorophyllide sters
Chromatography
Internal Standard
Pigment analysis
Internal Standard
Pigment analysis
Chlorophyllide sters
Chromatography
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Abstract •Easy and fast enzymatic synthesis of chlorophyllides a and b esters.•Dunalliela salina provided both the enzyme chlorophyllase and chlorophyll substrates.•Chromatographic properties of esters of different alcohols and diols were studied.•Chlorophyllide a (8′-hydroxyoctyl) ester was selected and completely characterized.•The proposed internal standard showed adequate stability over time. In this article the chlorophyllase activity of Dunaliella salina has been employed to generate different chlorophyllide a and b esters that could potentially be used as internal standards in pigment analysis. Chlorophyllide a (8’-hydroxyoctyl) ester was selected due its adequate chromatographic and spectral properties and was fully characterized by UV-Vis, ESI-MS and NMR. An easy room temperature procedure for its synthesis is described. Attachment
AbstractList •Easy and fast enzymatic synthesis of chlorophyllides a and b esters.•Dunalliela salina provided both the enzyme chlorophyllase and chlorophyll substrates.•Chromatographic properties of esters of different alcohols and diols were studied.•Chlorophyllide a (8′-hydroxyoctyl) ester was selected and completely characterized.•The proposed internal standard showed adequate stability over time. In this article the chlorophyllase activity of Dunaliella salina has been employed to generate different chlorophyllide a and b esters that could potentially be used as internal standards in pigment analysis. Chlorophyllide a (8’-hydroxyoctyl) ester was selected due its adequate chromatographic and spectral properties and was fully characterized by UV-Vis, ESI-MS and NMR. An easy room temperature procedure for its synthesis is described. Attachment
ArticleNumber 101688
Author Sanz, Noelia
Gavalás-Olea, Antonio
Vaz, Belén
Garrido, José Luis
Riobó, Pilar
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Cites_doi 10.3354/meps077183
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Snippet •Easy and fast enzymatic synthesis of chlorophyllides a and b esters.•Dunalliela salina provided both the enzyme chlorophyllase and chlorophyll...
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SubjectTerms Chlorophyllide sters
Chromatography
Internal Standard
Pigment analysis
Title Enzymatic synthesis and characterization of chlorophyllide derivatives as possible internal standards for pigment chromatographic analysis
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