Evolution of the active site of human glutathione transferase A2-2 for enhanced activity with dietary isothiocyanates - Zhang, Wei; Dourado, Daniel F.A.R.; Mannervik, Bengt (Biochimica et biophysica acta, 2015)
Keywords: active sites; biotransformation; Catalysis; Catalytic Domain; chemical defenses; Detoxication; Diet; Directed evolution; Enzyme engineering; evolutionary adaptation; excretion; food plants; glucosinolates; glutathione; Glutathione transferase; Glutathione Transferase - chemistry; Glutathione Transferase - metabolism; Humans; Isoenzymes - chemistry; Isoenzymes - metabolism; Isothiocyanate; isothiocyanates; Isothiocyanates - pharmacology; microorganisms; mutagenesis; mutants; neoplasms; phytophagous insects; protective effect; Substrate Specificity; thioglucosidase; tissues; toxicity; Detoxication; Isothiocyanate; Directed evolution; Enzyme engineering; Glutathione transferase