Magnetic poly(2-hydroxyethyl methacrylate) microspheres for affinity purification of monospecific anti-p46 kDa/Myo1C antibodies for early diagnosis of multiple sclerosis patients

• Published in: Bioscience reports Vol. 37; no. 2
• Main Authors: Horák, Daniel, Hlídková, Helena, Kit, Yurii, Antonyuk, Volodymyr, Myronovsky, Severyn, Stoika, Rostyslav
• Format: Journal Article
• Language: English
• Published: England Portland Press Ltd 30.04.2017
• Subjects: Animals; Humans; Immobilized Proteins - chemistry; Immobilized Proteins - immunology; Immunoglobulin G - immunology; Immunoglobulin G - isolation & purification; Magnetics - methods; Magnets - chemistry; Mice; Microspheres; Multiple Sclerosis - diagnosis; Multiple Sclerosis - immunology; Myosin Type I - chemistry; Myosin Type I - immunology; Polyhydroxyethyl Methacrylate - chemistry; Protein Isoforms - chemistry; Protein Isoforms - immunology; anti-p46 kDa/Myo1C; affinity purification; magnetic microspheres; multiple sclerosis

The aim of the present study is to develop new magnetic polymer microspheres with functional groups available for easy protein and antibody binding. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA-COOH) microspheres ~4 µm in diameter and containing ∼1 mmol COOH/g were synthesized b...