Magnetic poly(2-hydroxyethyl methacrylate) microspheres for affinity purification of monospecific anti-p46 kDa/Myo1C antibodies for early diagnosis of multiple sclerosis patients
The aim of the present study is to develop new magnetic polymer microspheres with functional groups available for easy protein and antibody binding. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA-COOH) microspheres ~4 µm in diameter and containing ∼1 mmol COOH/g were synthesized b...
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Published in | Bioscience reports Vol. 37; no. 2 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
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Portland Press Ltd
30.04.2017
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Abstract | The aim of the present study is to develop new magnetic polymer microspheres with functional groups available for easy protein and antibody binding. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA-COOH) microspheres ~4 µm in diameter and containing ∼1 mmol COOH/g were synthesized by multistep swelling polymerization of 2-hydroxyethyl methacrylate (HEMA), ethylene dimethacrylate (EDMA), and 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA), which was followed by MCMEMA hydrolysis. The microspheres were rendered magnetic by precipitation of iron oxide inside the pores, which made them easily separable in a magnetic field. Properties of the resulting magnetic poly(2-hydroxyethyl methacrylate) (mgt.PHEMA) particles with COOH functionality were examined by scanning and transmission electron microscopy (SEM and TEM), static volumetric adsorption of helium and nitrogen, mercury porosimetry, Fourier transform infrared (FTIR) and atomic absorption spectroscopy (AAS), and elemental analysis. Mgt.PHEMA microspheres were coupled with p46/Myo1C protein purified from blood serum of multiple sclerosis (MS) patients, which enabled easy isolation of monospecific anti-p46/Myo1C immunoglobulin G (IgG) antibodies from crude antibody preparations of mouse blood serum. High efficiency of this approach was confirmed by SDS/PAGE, Western blot, and dot blot analyses. The newly developed mgt.PHEMA microspheres conjugated with a potential disease biomarker, p46/Myo1C protein, are thus a promising tool for affinity purification of antibodies, which can improve diagnosis and treatment of MS patients. |
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AbstractList | The aim of the present study is to develop new magnetic polymer microspheres with functional groups available for easy protein and antibody binding. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA-COOH) microspheres ~4 µm in diameter and containing ∼1 mmol COOH/g were synthesized by multistep swelling polymerization of 2-hydroxyethyl methacrylate (HEMA), ethylene dimethacrylate (EDMA), and 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA), which was followed by MCMEMA hydrolysis. The microspheres were rendered magnetic by precipitation of iron oxide inside the pores, which made them easily separable in a magnetic field. Properties of the resulting magnetic poly(2-hydroxyethyl methacrylate) (mgt.PHEMA) particles with COOH functionality were examined by scanning and transmission electron microscopy (SEM and TEM), static volumetric adsorption of helium and nitrogen, mercury porosimetry, Fourier transform infrared (FTIR) and atomic absorption spectroscopy (AAS), and elemental analysis. Mgt.PHEMA microspheres were coupled with p46/Myo1C protein purified from blood serum of multiple sclerosis (MS) patients, which enabled easy isolation of monospecific anti-p46/Myo1C immunoglobulin G (IgG) antibodies from crude antibody preparations of mouse blood serum. High efficiency of this approach was confirmed by SDS/PAGE, Western blot, and dot blot analyses. The newly developed mgt.PHEMA microspheres conjugated with a potential disease biomarker, p46/Myo1C protein, are thus a promising tool for affinity purification of antibodies, which can improve diagnosis and treatment of MS patients. The aim of the present study is to develop new magnetic polymer microspheres with functional groups available for easy protein and antibody binding. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA-COOH) microspheres ~4 µm in diameter and containing ∼1 mmol COOH/g were synthesized by multistep swelling polymerization of 2-hydroxyethyl methacrylate (HEMA), ethylene dimethacrylate (EDMA), and 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA), which was followed by MCMEMA hydrolysis. The microspheres were rendered magnetic by precipitation of iron oxide inside the pores, which made them easily separable in a magnetic field. Properties of the resulting magnetic poly(2-hydroxyethyl methacrylate) (mgt.PHEMA) particles with COOH functionality were examined by scanning and transmission electron microscopy (SEM and TEM), static volumetric adsorption of helium and nitrogen, mercury porosimetry, Fourier transform infrared (FTIR) and atomic absorption spectroscopy (AAS), and elemental analysis. Mgt.PHEMA microspheres were coupled with p46/Myo1C protein purified from blood serum of multiple sclerosis (MS) patients, which enabled easy isolation of monospecific anti-p46/Myo1C immunoglobulin G (IgG) antibodies from crude antibody preparations of mouse blood serum. High efficiency of this approach was confirmed by SDS/PAGE, Western blot, and dot blot analyses. The newly developed mgt.PHEMA microspheres conjugated with a potential disease biomarker, p46/Myo1C protein, are thus a promising tool for affinity purification of antibodies, which can improve diagnosis and treatment of MS patients. The aim of the present study is to develop new magnetic polymer microspheres with functional groups available for easy protein and antibody binding. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA-COOH) microspheres ~4 [mu]m in diameter and containing -1 mmol COOH/g were synthesized by multistep swelling polymerization of 2-hydroxyethyl methacrylate (HEMA), ethylene dimethacrylate (EDMA), and 2-[(methoxycarbonyl)methoxy]ethyl methacrylate (MCMEMA), which was followed by MCMEMA hydrolysis. The microspheres were rendered magnetic by precipitation of iron oxide inside the pores, which made them easily separable in a magnetic field. Properties of the resulting magnetic poly(2-hydroxyethyl methacrylate) (mgt.PHEMA) particles with COOH functionality were examined by scanning and transmission electron microscopy (SEM and TEM), static volumetric adsorption of helium and nitrogen, mercury porosimetry, Fourier transform infrared (FTIR) and atomic absorption spectroscopy (AAS), and elemental analysis. Mgt.PHEMA microspheres were coupled with p46/Myo1C protein purified from blood serum of multiple sclerosis (MS) patients, which enabled easy isolation of monospecific anti-p46/Myo1C immunoglobulin G (IgG) antibodies from crude antibody preparations of mouse blood serum. High efficiency of this approach was confirmed by SDS/PAGE, Western blot, and dot blot analyses. The newly developed mgt.PHEMA microspheres conjugated with a potential disease biomarker, p46/Myo1C protein, are thus a promising tool for affinity purification of antibodies, which can improve diagnosis and treatment of MS patients. |
Author | Antonyuk, Volodymyr Myronovsky, Severyn Hlídková, Helena Kit, Yurii Horák, Daniel Stoika, Rostyslav |
Author_xml | – sequence: 1 givenname: Daniel surname: Horák fullname: Horák, Daniel email: horak@imc.cas.cz organization: Institute of Macromolecular Chemistry, AS CR, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic horak@imc.cas.cz – sequence: 2 givenname: Helena surname: Hlídková fullname: Hlídková, Helena organization: Institute of Macromolecular Chemistry, AS CR, Heyrovsky Sq. 2, 162 06 Prague 6, Czech Republic – sequence: 3 givenname: Yurii surname: Kit fullname: Kit, Yurii organization: Institute of Cell Biology, NAS of Ukraine, Drahomanov Str. 14/16, Lviv 79005, Ukraine – sequence: 4 givenname: Volodymyr surname: Antonyuk fullname: Antonyuk, Volodymyr organization: Institute of Cell Biology, NAS of Ukraine, Drahomanov Str. 14/16, Lviv 79005, Ukraine – sequence: 5 givenname: Severyn surname: Myronovsky fullname: Myronovsky, Severyn organization: Institute of Cell Biology, NAS of Ukraine, Drahomanov Str. 14/16, Lviv 79005, Ukraine – sequence: 6 givenname: Rostyslav surname: Stoika fullname: Stoika, Rostyslav organization: Institute of Cell Biology, NAS of Ukraine, Drahomanov Str. 14/16, Lviv 79005, Ukraine |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/28351895$$D View this record in MEDLINE/PubMed |
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Cites_doi | 10.1016/S0140-6736(14)61682-2 10.1016/j.biomaterials.2003.12.031 10.1002/app.26771 10.1346/CCMN.1988.0360105 10.1016/j.colsurfa.2015.11.013 10.1016/j.jchromb.2005.06.001 10.1039/B512605C 10.1135/cccc19661009 10.1002/apmc.1981.050950109 10.1016/S1381-1177(00)00223-X 10.1007/s00702-010-0543-z 10.1080/10601320802594774 10.1016/j.jpba.2009.06.011 10.1016/j.polymer.2009.03.023 10.1016/j.foodres.2009.01.020 10.1016/S0378-4347(99)00515-0 10.1039/C5RA09280A 10.1021/bp049896s 10.1002/mabi.201100393 10.1002/jbm.a.34297 10.1002/marc.200600409 10.1016/S0304-8853(02)00987-3 10.1016/0378-4347(95)00047-M 10.1351/pac199466081739 10.1016/j.msec.2013.09.009 10.1016/S0167-7799(01)01800-5 10.1074/mcp.M200066-MCP200 10.1016/j.jmmm.2005.01.038 10.1002/jctb.280350302 10.1002/macp.200290052 10.1002/ejic.201101066 10.1016/j.porgcoat.2015.05.023 10.1016/j.nano.2009.11.006 10.1016/S0926-860X(02)00348-4 10.1007/978-1-61779-052-2_6 10.1002/mabi.201400249 10.1088/0022-3727/36/13/202 10.1016/j.aca.2007.02.052 10.1016/S0003-2670(97)00262-6 10.1002/bit.21104 10.1016/j.jpha.2011.10.001 10.1016/j.biomaterials.2009.05.007 10.1016/j.ijpharm.2005.06.024 10.1016/S0142-9612(97)00059-8 10.1006/jssc.1997.7631 10.1002/msj.20242 10.1021/ja01176a018 10.1016/j.autrev.2015.07.014 |
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Keywords | anti-p46 kDa/Myo1C affinity purification magnetic microspheres multiple sclerosis |
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References | Adkins (2021111619092125100_B23) 2002; 1 Ugelstad (2021111619092125100_B30) 1983 Sidhu (2021111619092125100_B44) 1988; 36 Deng (2021111619092125100_B10) 2003; 257 Basti (2021111619092125100_B6) 2016; 490 Miller (2021111619092125100_B49) 2011; 78 Mollenhauer (2021111619092125100_B47) 2011; 118 Liu (2021111619092125100_B57) 2005; 822 Özkara (2021111619092125100_B52) 2004; 20 D’Ambrosio (2021111619092125100_B50) 2015; 14 Setchell (2021111619092125100_B1) 1985; 35 Wrotnowski (2021111619092125100_B25) 1998; 18 Ramirez (2021111619092125100_B11) 2003; 204 Yavuz (2021111619092125100_B21) 2009; 46 Štulík (2021111619092125100_B56) 1997; 352 Fang (2021111619092125100_B15) 2009; 50 Marhol (2021111619092125100_B33) 1976 Denizli (2021111619092125100_B53) 1995; 668 Horák (2021111619092125100_B31) 2012; 12 Silverstein (2021111619092125100_B43) 1991 Mou (2021111619092125100_B45) 2012; 2012 Rigby (2021111619092125100_B35) 2003; 238 Chen (2021111619092125100_B46) 1998; 137 Kuan (2021111619092125100_B17) 2014; 34 Arica (2021111619092125100_B16) 2000; 11 Kubinová (2021111619092125100_B28) 2009; 30 Horák (2021111619092125100_B27) 2015; 5 Schrader (2021111619092125100_B24) 2001; 19 Horák (2021111619092125100_B32) 2014; 14 Deyl (2021111619092125100_B55) 2000; 739 Jin (2021111619092125100_B12) 2009; 50 Bautista (2021111619092125100_B5) 2005; 293 Rittich (2021111619092125100_B54) 2009; 42 Wan (2021111619092125100_B8) 2006; 16 Rouquerol (2021111619092125100_B41) 1994; 66 Myronovkij (2021111619092125100_B26) 2016; 5 Wang (2021111619092125100_B7) 2006; 27 Ngaboni-Okassa (2021111619092125100_B9) 2005; 302 Horák (2021111619092125100_B39) 2013; 101 Taitt (2021111619092125100_B18) 2011; 726 Akgöl (2021111619092125100_B51) 2007; 106 Horák (2021111619092125100_B20) 1997; 18 Horák (2021111619092125100_B36) 2004; 25 Tartaj (2021111619092125100_B3) 2003; 36 Horák (2021111619092125100_B40) 1981; 95 Colthup (2021111619092125100_B42) 1990 Horák (2021111619092125100_B19) 1992; 86 Overberger (2021111619092125100_B29) 1949; 71 2021111619092125100_B34 Štamberg (2021111619092125100_B37) 1966; 31 Zhang (2021111619092125100_B13) 2007; 600 Taguchi (2021111619092125100_B2) 2007; 96 Zhang (2021111619092125100_B14) 2012; 2 GBD 2013 Mortality and Causes of Death Collaborators (2021111619092125100_B48) 2015; 385 Mallakpour (2021111619092125100_B4) 2015; 86 Chouhan (2021111619092125100_B22) 2010; 6 Kit (2021111619092125100_B38) 2010 |
References_xml | – volume: 385 start-page: 117 year: 2015 ident: 2021111619092125100_B48 article-title: Global, regional, and national age-sex specific all-cause and cause-specific mortality for 240 causes of death, 1990-2013: A systematic analysis for the Global Burden of Disease Study 2013 publication-title: Lancet doi: 10.1016/S0140-6736(14)61682-2 contributor: fullname: GBD 2013 Mortality and Causes of Death Collaborators – volume: 25 start-page: 5249 year: 2004 ident: 2021111619092125100_B36 article-title: Poly(2-hydroxyethyl methacrylate)-based slabs as a mouse embryonic stem cell support publication-title: Biomaterials doi: 10.1016/j.biomaterials.2003.12.031 contributor: fullname: Horák – volume: 106 start-page: 2405 year: 2007 ident: 2021111619092125100_B51 article-title: Pseudospecific magnetic affinity beads for immunoglobulin-g depletion from human serum publication-title: J. Appl. Polym. Sci. doi: 10.1002/app.26771 contributor: fullname: Akgöl – volume: 36 start-page: 31 year: 1988 ident: 2021111619092125100_B44 article-title: Transformation of trace element-substituted maghemite to hematite publication-title: Clay Clay Min doi: 10.1346/CCMN.1988.0360105 contributor: fullname: Sidhu – volume: 490 start-page: 222 year: 2016 ident: 2021111619092125100_B6 article-title: Surface modification of γ-Fe2O3 nanoparticles by grafting from poly-(hydroxyethylmethacrylate) and poly-(methacrylic acid): qualitative and quantitative analysis of the polymeric coating publication-title: Colloid Surf. A doi: 10.1016/j.colsurfa.2015.11.013 contributor: fullname: Basti – volume: 5 start-page: 175 year: 2016 ident: 2021111619092125100_B26 article-title: Identification of a 48 kDa form of unconventional myosin 1c in blood serum of patients with autoimmune diseases publication-title: Biochem. Biophys. Rep. contributor: fullname: Myronovkij – volume: 822 start-page: 91 year: 2005 ident: 2021111619092125100_B57 article-title: Immobilization of lipase onto micron-size magnetic beads publication-title: J. Chromatogr. B doi: 10.1016/j.jchromb.2005.06.001 contributor: fullname: Liu – volume: 16 start-page: 298 year: 2006 ident: 2021111619092125100_B8 article-title: Size-controlled preparation of magnetite nanoparticles in the presence of graft copolymers publication-title: J. Mater. Chem. doi: 10.1039/B512605C contributor: fullname: Wan – start-page: 75 volume-title: Ion Exchangers in Chemistry and Radiochemistry year: 1976 ident: 2021111619092125100_B33 contributor: fullname: Marhol – volume: 31 start-page: 1009 year: 1966 ident: 2021111619092125100_B37 article-title: Chemical transformations of polymers III. Selective hydrolysis of a copolymer of diethylene glycol methacrylate and diethylene glycol dimethacrylate publication-title: Collect. Czech. Chem. Commun. doi: 10.1135/cccc19661009 contributor: fullname: Štamberg – volume: 95 start-page: 117 year: 1981 ident: 2021111619092125100_B40 article-title: Reactive polymers XXXVI. The effect of polymerization conditions on the porosity and mechanical properties of macroporous suspension copolymers from glycidyl methacrylate-ethylene dimethacrylate publication-title: Angew. Makromol. Chem. doi: 10.1002/apmc.1981.050950109 contributor: fullname: Horák – volume: 11 start-page: 127 year: 2000 ident: 2021111619092125100_B16 article-title: Immobilization of glucoamylase onto spacer-arm attached magnetic poly(methylmethacrylate) microspheres: Characterization and application to a continuous flow reactor publication-title: J. Mol. Catal. B-Enzym. doi: 10.1016/S1381-1177(00)00223-X contributor: fullname: Arica – ident: 2021111619092125100_B34 – volume: 118 start-page: 691 year: 2011 ident: 2021111619092125100_B47 article-title: Different CSF β-amyloid processing in Alzheimer's and Creutzfeldt-Jakob disease publication-title: J. Neural Transm. doi: 10.1007/s00702-010-0543-z contributor: fullname: Mollenhauer – volume: 46 start-page: 163 year: 2009 ident: 2021111619092125100_B21 article-title: Concanavalin A binding on PHEMA beads and their interactions with myeloma cells publication-title: J. Macromol. Sci. Part A-Pure Appl. Chem. doi: 10.1080/10601320802594774 contributor: fullname: Yavuz – volume: 50 start-page: 891 year: 2009 ident: 2021111619092125100_B12 article-title: Magnetic particle-based chemiluminescence enzyme immunoassay for free thyroxine in human serum publication-title: J. Pharm. Biomed. Anal. doi: 10.1016/j.jpba.2009.06.011 contributor: fullname: Jin – volume: 50 start-page: 2290 year: 2009 ident: 2021111619092125100_B15 article-title: Synthesis of core-shell structured PS/Fe3O4 microbeads and their magnetorheology publication-title: Polymer doi: 10.1016/j.polymer.2009.03.023 contributor: fullname: Fang – volume: 42 start-page: 493 year: 2009 ident: 2021111619092125100_B54 article-title: Functionalized magnetic microspheres with hydrophilic properties for molecular diagnostic applications publication-title: Food Res. Int. doi: 10.1016/j.foodres.2009.01.020 contributor: fullname: Rittich – volume: 739 start-page: 3 year: 2000 ident: 2021111619092125100_B55 article-title: Advanced separation methods for collagen parent α-chains, their polymers and fragments publication-title: J. Chromatogr. B doi: 10.1016/S0378-4347(99)00515-0 contributor: fullname: Deyl – volume: 5 start-page: 63050 year: 2015 ident: 2021111619092125100_B27 article-title: Calf thymus histone-conjugated magnetic poly(2-oxoethyl methacrylate) microspheres for affinity isolation of anti-histone IgGs from the blood serum of patients with systemic lupus erythematosus publication-title: RSC Adv. doi: 10.1039/C5RA09280A contributor: fullname: Horák – volume: 20 start-page: 1169 year: 2004 ident: 2021111619092125100_B52 article-title: A novel magnetic adsorbent for immunoglobulin-G purification in a magnetically stabilized fluidized bed publication-title: Biotechnol. Prog. doi: 10.1021/bp049896s contributor: fullname: Özkara – volume: 12 start-page: 647 year: 2012 ident: 2021111619092125100_B31 article-title: New monodisperse magnetic polymer microspheres biofunctionalized for enzyme catalysis and bioaffinity separations publication-title: Macromol. Biosci. doi: 10.1002/mabi.201100393 contributor: fullname: Horák – volume: 101 start-page: 23 year: 2013 ident: 2021111619092125100_B39 article-title: Albumin-coated monodisperse magnetic poly(glycidyl methacrylate) microspheres with immobilized antibodies: Application to the capture of epithelial cancer cells publication-title: J. Biomed. Mater. Res. Part A doi: 10.1002/jbm.a.34297 contributor: fullname: Horák – volume: 27 start-page: 1665 year: 2006 ident: 2021111619092125100_B7 article-title: Surface functionalization of Fe3O4 magnetic nanoparticles via RAFT-mediated graft polymerization publication-title: Macromol. Rapid Commun. doi: 10.1002/marc.200600409 contributor: fullname: Wang – volume: 257 start-page: 69 year: 2003 ident: 2021111619092125100_B10 article-title: Preparation of magnetic polymeric particles via inverse microemulsion polymerization process publication-title: J. Magn. Magn. Mater. doi: 10.1016/S0304-8853(02)00987-3 contributor: fullname: Deng – volume: 668 start-page: 13 year: 1995 ident: 2021111619092125100_B53 article-title: Protein A immobilized polyhydroxyethylmethacrylate beads for affinity sorption of human immunoglobulin G publication-title: J. Chromatogr. B doi: 10.1016/0378-4347(95)00047-M contributor: fullname: Denizli – year: 1983 ident: 2021111619092125100_B30 article-title: Magnetic polymer particles and process for the preparation thereof publication-title: Eur. Pat. contributor: fullname: Ugelstad – volume: 66 start-page: 1739 year: 1994 ident: 2021111619092125100_B41 article-title: Recommendations for the characterization of porous solids publication-title: Pure Appl. Chem. doi: 10.1351/pac199466081739 contributor: fullname: Rouquerol – volume: 34 start-page: 193 year: 2014 ident: 2021111619092125100_B17 article-title: Immunocapture of CD133-positive cells from human cancer cell lines by using monodisperse magnetic poly(glycidyl methacrylate) microspheres containing amino groups publication-title: Mater. Sci. Eng. C doi: 10.1016/j.msec.2013.09.009 contributor: fullname: Kuan – volume: 19 start-page: S55 year: 2001 ident: 2021111619092125100_B24 article-title: Peptidomics technologies for human body fluids publication-title: Trends Biotechnol. doi: 10.1016/S0167-7799(01)01800-5 contributor: fullname: Schrader – volume: 1 start-page: 947 year: 2002 ident: 2021111619092125100_B23 article-title: Toward a human blood serum proteome: analysis by multidimensional separation coupled with mass spectrometry publication-title: Mol. Cell. Proteomics doi: 10.1074/mcp.M200066-MCP200 contributor: fullname: Adkins – volume: 293 start-page: 20 year: 2005 ident: 2021111619092125100_B5 article-title: Surface characterization of dextran-coated iron oxide nanoparticles prepared by laser pyrolysis and coprecipitation publication-title: J. Magn. Magn. Mater. doi: 10.1016/j.jmmm.2005.01.038 contributor: fullname: Bautista – volume: 35 start-page: 175 year: 1985 ident: 2021111619092125100_B1 article-title: Magnetic separations in biotechnology: a review publication-title: J. Chem. Technol. Biotechnol. doi: 10.1002/jctb.280350302 contributor: fullname: Setchell – volume-title: Spectrometric Identification of Organic Compounds year: 1991 ident: 2021111619092125100_B43 contributor: fullname: Silverstein – volume: 204 start-page: 22 year: 2003 ident: 2021111619092125100_B11 article-title: Magnetic polystyrene nanoparticles with a high magnetite content obtained by miniemulsion processes publication-title: Macromol. Chem. Phys. doi: 10.1002/macp.200290052 contributor: fullname: Ramirez – start-page: 209 volume-title: Biocompatible Nanomaterials: Synthesis, Characterization and Applications year: 2010 ident: 2021111619092125100_B38 article-title: Immunogenicity and adjuvant properties of novel biocompatible nanoparticles contributor: fullname: Kit – volume: 2012 start-page: 2684 year: 2012 ident: 2021111619092125100_B45 article-title: Crystal-phase- and morphology-controlled synthesis of Fe2O3 nanomaterials publication-title: Eur. J. Inorg. Chem. doi: 10.1002/ejic.201101066 contributor: fullname: Mou – volume: 86 start-page: 194 year: 2015 ident: 2021111619092125100_B4 article-title: A review of current coupling agents for modification of metal oxide nanoparticles publication-title: Prog. Org. Coat. doi: 10.1016/j.porgcoat.2015.05.023 contributor: fullname: Mallakpour – volume: 6 start-page: 453 year: 2010 ident: 2021111619092125100_B22 article-title: Release dynamics of ciprofloxacin from swellable nanocarriers of poly(2-hydroxyethyl methacrylate): An in vitro study publication-title: Nanomed.-Nanotechnol. Biol. Med. doi: 10.1016/j.nano.2009.11.006 contributor: fullname: Chouhan – volume: 18 start-page: 14 year: 1998 ident: 2021111619092125100_B25 article-title: The future of plasma proteins publication-title: Genet. Eng. News contributor: fullname: Wrotnowski – volume: 238 start-page: 303 year: 2003 ident: 2021111619092125100_B35 article-title: Interpreting mercury porosimetry data for catalyst supports using semi-empirical alternatives to the Washburn equation publication-title: Appl. Catal. A doi: 10.1016/S0926-860X(02)00348-4 contributor: fullname: Rigby – volume: 726 start-page: 77 year: 2011 ident: 2021111619092125100_B18 article-title: Surface modification and biomolecule immobilization on polymer spheres for biosensing applications publication-title: Methods Mol. Biol. doi: 10.1007/978-1-61779-052-2_6 contributor: fullname: Taitt – volume: 14 start-page: 1590 year: 2014 ident: 2021111619092125100_B32 article-title: Monodisperse carboxyl-functionalized poly(ethylene glycol)-coated magnetic poly(glycidyl methacrylate) microspheres: Application to the immunocapture of β-amyloid peptides publication-title: Macromol. Biosci. doi: 10.1002/mabi.201400249 contributor: fullname: Horák – volume: 86 start-page: 681 year: 1992 ident: 2021111619092125100_B19 article-title: The use of poly(2-hydroxyethyl methacrylate) in medicine publication-title: Chem. Listy contributor: fullname: Horák – volume: 36 start-page: R182 year: 2003 ident: 2021111619092125100_B3 article-title: The preparation of magnetic nanoparticles for applications in biomedicine publication-title: J. Phys. D-Appl. Phys. doi: 10.1088/0022-3727/36/13/202 contributor: fullname: Tartaj – volume: 600 start-page: 105 year: 2007 ident: 2021111619092125100_B13 article-title: Sequential injection chemiluminescence immunoassay for nonionic surfactants by using magnetic microbeads publication-title: Anal. Chim. Acta doi: 10.1016/j.aca.2007.02.052 contributor: fullname: Zhang – volume: 352 start-page: 1 year: 1997 ident: 2021111619092125100_B56 article-title: Stationary phases for peptide analysis by high performance liquid chromatography: A review publication-title: Anal. Chim. Acta doi: 10.1016/S0003-2670(97)00262-6 contributor: fullname: Štulík – volume: 96 start-page: 272 year: 2007 ident: 2021111619092125100_B2 article-title: Detection of Cryptosporidium parvum oocysts using a microfluidic device equipped with the SUS micromesh and FITC-labeled antibody publication-title: Biotechnol. Bioeng. doi: 10.1002/bit.21104 contributor: fullname: Taguchi – volume-title: Introduction to Infrared and Raman Spectroscopy year: 1990 ident: 2021111619092125100_B42 contributor: fullname: Colthup – volume: 2 start-page: 130 year: 2012 ident: 2021111619092125100_B14 article-title: Comparison of chemiluminescence enzyme immunoassay based on magnetic microparticles with traditional colorimetric ELISA for the detection of serum α-fetoprotein publication-title: J. Pharm. Anal. doi: 10.1016/j.jpha.2011.10.001 contributor: fullname: Zhang – volume: 30 start-page: 4601 year: 2009 ident: 2021111619092125100_B28 article-title: Cholesterol-modified superporous poly(2-hydroxyethyl methacrylate) scaffolds for tissue engineering publication-title: Biomaterials doi: 10.1016/j.biomaterials.2009.05.007 contributor: fullname: Kubinová – volume: 302 start-page: 187 year: 2005 ident: 2021111619092125100_B9 article-title: Development and characterization of sub-micron poly(D,L-lactide-co-glycolide) particles loaded with magnetite/maghemite nanoparticles publication-title: Int. J. Pharm. doi: 10.1016/j.ijpharm.2005.06.024 contributor: fullname: Ngaboni-Okassa – volume: 18 start-page: 1355 year: 1997 ident: 2021111619092125100_B20 article-title: Hydrogels in endovascular embolization VI. Toxicity tests of poly(2-hydroxyethyl methacrylate) particles on cell cultures publication-title: Biomaterials doi: 10.1016/S0142-9612(97)00059-8 contributor: fullname: Horák – volume: 137 start-page: 185 year: 1998 ident: 2021111619092125100_B46 article-title: Hydrothermal synthesis and characterization of nanocrystalline γ-Fe2O3 particles publication-title: J. Solid State Chem. doi: 10.1006/jssc.1997.7631 contributor: fullname: Chen – volume: 78 start-page: 268 year: 2011 ident: 2021111619092125100_B49 article-title: Multiple sclerosis: where will we be in 2020 publication-title: Mt. Sinai J. Med. doi: 10.1002/msj.20242 contributor: fullname: Miller – volume: 71 start-page: 2661 year: 1949 ident: 2021111619092125100_B29 article-title: The preparation of some aliphatic azo nitriles and their decomposition in solution publication-title: J. Am. Chem. Soc. doi: 10.1021/ja01176a018 contributor: fullname: Overberger – volume: 14 start-page: 1097 year: 2015 ident: 2021111619092125100_B50 article-title: Peripheral blood biomarkers in multiple sclerosis publication-title: Autoimmun. Rev. doi: 10.1016/j.autrev.2015.07.014 contributor: fullname: D’Ambrosio |
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SubjectTerms | Animals Humans Immobilized Proteins - chemistry Immobilized Proteins - immunology Immunoglobulin G - immunology Immunoglobulin G - isolation & purification Magnetics - methods Magnets - chemistry Mice Microspheres Multiple Sclerosis - diagnosis Multiple Sclerosis - immunology Myosin Type I - chemistry Myosin Type I - immunology Polyhydroxyethyl Methacrylate - chemistry Protein Isoforms - chemistry Protein Isoforms - immunology |
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Title | Magnetic poly(2-hydroxyethyl methacrylate) microspheres for affinity purification of monospecific anti-p46 kDa/Myo1C antibodies for early diagnosis of multiple sclerosis patients |
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