Anti–Human Neutrophil Antigen-3a Induced Transfusion-Related Acute Lung Injury in Mice by Direct Disturbance of Lung Endothelial Cells

• Published in: Arteriosclerosis, thrombosis, and vascular biology Vol. 33; no. 11; pp. 2538 - 2548
• Main Authors: Bayat, Behnaz, Tjahjono, Yudy, Sydykov, Akylbek, Werth, Silke, Hippenstiel, Stefan, Weissmann, Nobert, Sachs, Ulrich J., Santoso, Sentot
• Format: Journal Article
• Language: English
• Published: United States American Heart Association, Inc 01.11.2013
• Subjects: Actins - metabolism; Acute Lung Injury - etiology; Acute Lung Injury - immunology; Animals; Antibodies - immunology; Antibodies - pharmacology; Bronchoalveolar Lavage Fluid - cytology; Bronchoalveolar Lavage Fluid - immunology; Capillary Permeability - immunology; Disease Models, Animal; Endothelium, Vascular - immunology; Endothelium, Vascular - metabolism; Humans; Isoantigens - immunology; Lipopolysaccharides - pharmacology; Membrane Glycoproteins - genetics; Membrane Glycoproteins - immunology; Membrane Transport Proteins - immunology; Membrane Transport Proteins - metabolism; Mice; Mice, Knockout; NADPH Oxidase 2; NADPH Oxidases - genetics; Neutrophils - immunology; Respiratory Mucosa - cytology; Respiratory Mucosa - immunology; Transfusion Reaction; endothelium; acute lung injury; reactive oxygen species

OBJECTIVE—Antibodies against human neutrophil antigen-3a (HNA-3a) located on choline transporter-like protein 2 induce severe transfusion-related acute lung injury (TRALI). This study aims to identify the mechanism implicated in anti–HNA-3a-mediated TRALI. APPROACH AND RESULTS—Our analysis shows tha...