Quantitative spectroscopy analysis of prokaryotic cells: vegetative cells and spores
Multiwavelength ultraviolet/visible (UV-Vis) spectra of microorganisms and cell suspensions contain quantitative information on properties such as number, size, shape, chemical composition, and internal structure of the suspended particles. These properties are essential for the identification and c...
Saved in:
Published in | Biosensors & bioelectronics Vol. 19; no. 8; pp. 893 - 903 |
---|---|
Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Lausanne
Elsevier B.V
15.03.2004
Elsevier Science |
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Multiwavelength ultraviolet/visible (UV-Vis) spectra of microorganisms and cell suspensions contain quantitative information on properties such as number, size, shape, chemical composition, and internal structure of the suspended particles. These properties are essential for the identification and classification of microorganisms and cells. The complexity of microorganisms in terms of their chemical composition and internal structure make the interpretation of their spectral signature a difficult task. In this paper, a model is proposed for the quantitative interpretation of spectral patterns resulting from transmission measurements of prokaryotic microorganism suspensions. It is also demonstrated that different organisms give rise to spectral differences that may be used for their identification and classification. The proposed interpretation model is based on light scattering theory, spectral deconvolution techniques, and on the approximation of the frequency dependent optical properties of the basic constituents of living organisms. The quantitative deconvolution in terms of the interpretation model yields critical information necessary for the detection and identification of microorganisms, such as size, dry mass, dipicolinic acid concentration, nucleotide concentration, and an average representation of the internal scattering elements of the organisms.
E. coli,
P. agglomerans,
B. subtilis spores, and vegetative cells and spores of
Bacillus globigii are used as case studies. It is concluded that spectroscopy techniques coupled with effective interpretation models are applicable to a wide range of cell types found in diverse environments. |
---|---|
AbstractList | Multiwavelength ultraviolet/visible (UV-Vis) spectra of microorganisms and cell suspensions contain quantitative information on properties such as number, size, shape, chemical composition, and internal structure of the suspended particles. These properties are essential for the identification and classification of microorganisms and cells. The complexity of microorganisms in terms of their chemical composition and internal structure make the interpretation of their spectral signature a difficult task. In this paper, a model is proposed for the quantitative interpretation of spectral patterns resulting from transmission measurements of prokaryotic microorganism suspensions. It is also demonstrated that different organisms give rise to spectral differences that may be used for their identification and classification. The proposed interpretation model is based on light scattering theory, spectral deconvolution techniques, and on the approximation of the frequency dependent optical properties of the basic constituents of living organisms. The quantitative deconvolution in terms of the interpretation model yields critical information necessary for the detection and identification of microorganisms, such as size, dry mass, dipicolinic acid concentration, nucleotide concentration, and an average representation of the internal scattering elements of the organisms. E. coli, P. agglomerans, B. subtilis spores, and vegetative cells and spores of Bacillus globigii are used as case studies. It is concluded that spectroscopy techniques coupled with effective interpretation models are applicable to a wide range of cell types found in diverse environments. Multiwavelength ultraviolet/visible (UV-Vis) spectra of microorganisms and cell suspensions contain quantitative information on properties such as number, size, shape, chemical composition, and internal structure of the suspended particles. These properties are essential for the identification and classification of microorganisms and cells. The complexity of microorganisms in terms of their chemical composition and internal structure make the interpretation of their spectral signature a difficult task. In this paper, a model is proposed for the quantitative interpretation of spectral patterns resulting from transmission measurements of prokaryotic microorganism suspensions. It is also demonstrated that different organisms give rise to spectral differences that may be used for their identification and classification. The proposed interpretation model is based on light scattering theory, spectral deconvolution techniques, and on the approximation of the frequency dependent optical properties of the basic constituents of living organisms. The quantitative deconvolution in terms of the interpretation model yields critical information necessary for the detection and identification of microorganisms, such as size, dry mass, dipicolinic acid concentration, nucleotide concentration, and an average representation of the internal scattering elements of the organisms. E. coli, P. agglomerans, B. subtilis spores, and vegetative cells and spores of Bacillus globigii are used as case studies. It is concluded that spectroscopy techniques coupled with effective interpretation models are applicable to a wide range of cell types found in diverse environments. |
Author | Olivares, Jose A Garcı́a-Rubio, Luis H Alupoaei, Catalina E |
Author_xml | – sequence: 1 givenname: Catalina E surname: Alupoaei fullname: Alupoaei, Catalina E organization: Department of Chemical Engineering, University of South Florida, Tampa, FL 33620, USA – sequence: 2 givenname: Jose A surname: Olivares fullname: Olivares, Jose A organization: Bioscience Division, Los Alamos National Laboratory, Los Alamos, Mexico – sequence: 3 givenname: Luis H surname: Garcı́a-Rubio fullname: Garcı́a-Rubio, Luis H email: garcia@marine.usf.edu organization: Department of Chemical Engineering, University of South Florida, Tampa, FL 33620, USA |
BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=15474568$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/15128109$$D View this record in MEDLINE/PubMed |
BookMark | eNqFkcFq3DAQhkVISTZpX6CH4kt7szMjS7JceimhbQKBEkjOQpbHRVuvtZW8C_v21XYN7ak5CYnv_5n5dMXOpzARY28RKgRUN-uq8yFVHKCuQFfA8YytUDd1KXgtz9kKWqlKqVR9ya5SWgNAgy1csEuUyDVCu2JPjzs7zX62s99Tkbbk5hiSC9tDYSc7HpJPRRiKbQw_bTyE2bvC0Timj8WeftAS-_OS-T4XhEjpNXs12DHRm-W8Zs9fvzzd3pUP37_d335-KJ3Aei4H3fSNlmKAVjQO7CC4gHwlCVxrlIo0ccTOCauscl3dDlw4p1DyzvG-qa_Zh1NvHu_XjtJsNj4dh7EThV0yeVvkbS1eBLHRXHGJGeQn0GULKdJgttFv8uYGwRylm7U5SjdH6Qa0ydJz6N3Svus21P-NLJYz8H4BbHJ2HKKdnE__cKIRUunMfTpxlKXtPUWTnKfJUe9j_hjTB_-_OX4D95aiUQ |
CitedBy_id | crossref_primary_10_1002_cjce_20095 crossref_primary_10_1364_BOE_2_002126 crossref_primary_10_1088_0967_3334_35_5_793 crossref_primary_10_1039_B9AY00185A crossref_primary_10_1111_j_1472_765X_2008_02461_x crossref_primary_10_1364_AO_49_000180 crossref_primary_10_1016_j_bios_2013_09_015 crossref_primary_10_1080_10402004_2011_653871 crossref_primary_10_1016_j_saa_2020_119423 crossref_primary_10_1080_01919512_2015_1079119 crossref_primary_10_1155_2017_8365189 crossref_primary_10_1364_AO_46_001554 crossref_primary_10_1364_OE_16_018215 crossref_primary_10_1016_j_hal_2008_05_001 crossref_primary_10_1039_D1AN00212K crossref_primary_10_1002_bit_27664 crossref_primary_10_1039_B702920A crossref_primary_10_1039_D1RA01316E crossref_primary_10_1016_j_foodres_2018_09_026 crossref_primary_10_1063_1_1819520 crossref_primary_10_1155_2017_4039048 crossref_primary_10_3390_fluids2010009 crossref_primary_10_1016_j_colsurfa_2004_08_046 crossref_primary_10_1016_j_ab_2021_114216 crossref_primary_10_1039_C9AY01339C crossref_primary_10_1134_S1024856011020114 crossref_primary_10_1103_PhysRevLett_97_157402 crossref_primary_10_1111_j_1745_4581_2007_00077_x crossref_primary_10_1063_1_4745785 crossref_primary_10_1128_CMR_18_4_583_607_2005 |
Cites_doi | 10.1099/00221287-16-2-418 10.1016/B978-0-12-404550-7.50008-7 10.1063/1.3060205 10.1016/0003-2697(83)90624-3 10.1117/1.429979 10.1063/1.1681724 10.1364/AO.7.001879 10.1364/AO.36.002818 10.1016/0021-9797(78)90140-6 10.1016/0022-5193(68)90174-4 10.1562/0031-8655(2000)071<0610:LSAAMF>2.0.CO;2 10.1117/12.418743 10.1016/0022-5193(72)90087-2 10.1063/1.555743 10.1086/150207 |
ContentType | Journal Article |
Copyright | 2003 Elsevier B.V. 2004 INIST-CNRS |
Copyright_xml | – notice: 2003 Elsevier B.V. – notice: 2004 INIST-CNRS |
DBID | IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QO 8FD FR3 P64 7X8 |
DOI | 10.1016/j.bios.2003.08.021 |
DatabaseName | Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Biotechnology Research Abstracts Technology Research Database Engineering Research Database Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Engineering Research Database Biotechnology Research Abstracts Technology Research Database Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
DatabaseTitleList | MEDLINE MEDLINE - Academic Engineering Research Database |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Engineering Biology |
EISSN | 1873-4235 |
EndPage | 903 |
ExternalDocumentID | 10_1016_j_bios_2003_08_021 15128109 15474568 S0956566303003002 |
Genre | Validation Studies Research Support, U.S. Gov't, Non-P.H.S Evaluation Studies Research Support, Non-U.S. Gov't Journal Article |
GroupedDBID | --- --K --M .HR .~1 0R~ 1B1 1RT 1~. 1~5 23N 4.4 457 4G. 53G 5GY 5VS 7-5 71M 8P~ 9JM 9JN AABNK AACTN AAEDT AAEDW AAIAV AAIKJ AAKOC AALRI AAOAW AAQFI AAQXK AARLI AAXUO ABFNM ABGSF ABJNI ABMAC ABUDA ABXDB ABYKQ ACDAQ ACGFS ACNNM ACRLP ADBBV ADECG ADEZE ADMUD ADTZH ADUVX AEBSH AECPX AEHWI AEKER AENEX AFFNX AFKWA AFTJW AFXIZ AFZHZ AGHFR AGRDE AGUBO AGYEJ AHHHB AHJVU AIEXJ AIKHN AITUG AJBFU AJOXV AJQLL AJSZI ALMA_UNASSIGNED_HOLDINGS AMFUW AMRAJ ASPBG AVWKF AXJTR AZFZN BJAXD BKOJK BLXMC CS3 DOVZS DU5 EBS EFJIC EFLBG EJD EO8 EO9 EP2 EP3 F5P FDB FEDTE FGOYB FIRID FLBIZ FNPLU FYGXN G-2 G-Q GBLVA HLW HMU HVGLF HZ~ IHE J1W JJJVA KOM LX3 M36 M41 MO0 N9A O-L O9- OAUVE OZT P-8 P-9 P2P PC. Q38 R2- RIG ROL RPZ SBG SCB SCC SCH SDF SDG SDP SES SEW SPC SPCBC SSK SST SSU SSZ T5K TN5 WUQ XFK XPP Y6R YK3 ZMT ~G- ~KM AAPBV ABPIF ABPTK IQODW AAHBH AAXKI AKRWK CGR CUY CVF ECM EIF NPM AAYXX AFJKZ CITATION 7QO 8FD FR3 P64 7X8 |
ID | FETCH-LOGICAL-c413t-f87d7854f0947c0af424054fe50288156e8e211bc4a6a6cb39f24cc6152bc2d73 |
IEDL.DBID | AIKHN |
ISSN | 0956-5663 |
IngestDate | Fri Aug 16 22:52:17 EDT 2024 Thu Aug 15 23:19:50 EDT 2024 Thu Sep 26 18:03:04 EDT 2024 Sat Sep 28 08:50:43 EDT 2024 Sun Oct 22 16:09:01 EDT 2023 Fri Feb 23 02:18:19 EST 2024 |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 8 |
Keywords | Multiwavelength spectroscopy Absorbance Microorganisms Biosensor Scattering Optical properties Spectrometry Microorganism Spores Quantitative analysis |
Language | English |
License | CC BY 4.0 |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c413t-f87d7854f0947c0af424054fe50288156e8e211bc4a6a6cb39f24cc6152bc2d73 |
Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
PMID | 15128109 |
PQID | 17826251 |
PQPubID | 23462 |
PageCount | 11 |
ParticipantIDs | proquest_miscellaneous_71912934 proquest_miscellaneous_17826251 crossref_primary_10_1016_j_bios_2003_08_021 pubmed_primary_15128109 pascalfrancis_primary_15474568 elsevier_sciencedirect_doi_10_1016_j_bios_2003_08_021 |
PublicationCentury | 2000 |
PublicationDate | 2004-03-15 |
PublicationDateYYYYMMDD | 2004-03-15 |
PublicationDate_xml | – month: 03 year: 2004 text: 2004-03-15 day: 15 |
PublicationDecade | 2000 |
PublicationPlace | Lausanne |
PublicationPlace_xml | – name: Lausanne – name: England |
PublicationTitle | Biosensors & bioelectronics |
PublicationTitleAlternate | Biosens Bioelectron |
PublicationYear | 2004 |
Publisher | Elsevier B.V Elsevier Science |
Publisher_xml | – name: Elsevier B.V – name: Elsevier Science |
References | Veshkin, N.L., 1999. Photonics of Polymers. Moscow University Press, Moscow. Wyatt, Phillips (BIB32) 1972; 37 Mattley, Leparc, Potter, Garcia-Rubio (BIB17) 2000; 71 Koch (BIB13) 1968; 18 Bronk, B.V., van de Merwe, W.P., Huffman, D.R., 1991. In: Nelson, W.H. (Ed.), Polarized Light Scattering as Means for Detecting Subtle Changes in Microbial Populations, in Modern Techniques for Rapid Microbiological Analysis. VCH Publishers Inc., New York, pp. 171–197. Doi, R.H., 1969. Changes in neuclic acids during sporulation. In: Gould, G.W., Hurst, A. (Eds.), The Bacterial Spores, Academic Press, New York, NY (Chapter 4). Bohren, C.F., Huffman, D.F., 1983. Absorption and Scattering of Light by Small Particles. Wiley, New York. Flatau, P., 1992. Scattering by Irregular Particles in Anomalous Diffraction and Discrete Dipole Approximations. Ph.D. Thesis. Colorado State University, Colorado. Kerker, M., 1969. The Scattering of Light and Other Electromagnetic Radiation. Pergamon Press, New York. Ross, Billing (BIB23) 1957; 16 Thormälen, Straub, Grigull (BIB25) 1985; 14 Wiscombe, W.J., 1979. Mie Scattering Calculations: Advances in Technique and Fast, Vector-Speed Computer Codes, NCAR/TN-140 + STR. National Center for Atmospheric Research, Boulder, Colorado. Alupoaei, C.E., Olivares, J.A., Garcı́a-Rubio, L.H., 2002. Quantitative Analysis of Prokaryotic Cells: Vegetative Cells and Spores. Report No. LA-UR026586. Los Alamos National Laboratory, Los Alamos, MN. Newman, C.D., Hebner, B.T., Allen, F.S., 1991. A polarization-sensitive multiparameter light-scattering characterization of bacteria. In: Nelson, W.H. (Ed.), Modern Techniques for Rapid Microbiological Analysis. VCH, New York, NY, pp. 145–169. Johnson, B.L., Smith, J., 1972. Refractive index and densities of some common polymer solvents. In: Huglin, M.B. (Ed.), Light Scattering from Polymer Solutions. Academic Press, New York, NY (Chapter 2). Warth (BIB29) 1983; 130 Tuminello, Arakawa, Khare, Wrobel, Querry, Milham (BIB26) 1997; 36 Wyatt (BIB31) 1968; 7 Mourant, Campolat, Brocke, Esponda-Ramos, Johnson, Matanock, Freyer (BIB18) 2000; 5 Latimer, Barber (BIB14) 1977; 63 Huglin, M.B., 1972. Specific refractive index increments. In: Huglin, M.B. (Ed.), Light Scattering from Polymer Solutions. Academic Press, New York (Chapter 6). Purcell (BIB21) 1969; 158 Sambrook, J., Fritsch, E.F., Maniatis, T., 1989. Spectrophotometric determination of the amount of DNA and RNA. In : Molecular Cloning, 2nd ed. Cold Springs Harbor Laboratory Press (Appendix E.5). Lopatin, V.N., Sidko, F.Ya., 1988. Introduction to Optics of Cell Suspensions. Nauka, Moscow. Freifelder, D., 1982. In: Physical Biochemistry, second ed. Freeman, New York, Chapter 14, p. 504. van de Hulst, H.C., 1957. Light Scattering by Small Particles. Wiley, New York. Alupoaei, C.E., 2001. Modeling of the Transmission Spectra of Microorganisms. Master of Science Thesis. University of South Florida. Mattley, Y., Garcia-Rubio, L.H., 2000. Multiwavelength spectroscopy for the detection, identification and quantification of cells. In: Proceedings of the SPIE National Meeting. Boston, Massachusetts, 5–8 November. Murrell, W.G., 1969. Chemical composition of spores and spore structures. In: Gould, G.W., Hurst, A. (Eds.), The Bacterial Spores. Academic Press, New York, NY (Chapter 7). Reynolds, L.O., 1975. Optical Diffuse Reflectance and Transmittance from an Anisotropically Scattering Finite Blood Medium. Ph.D. Dissertation. University of Washington. Adams, R.L., Knowler, J.T., Leader, D.P., 1989. The Biochemistry of Nucleic Acids, 10th ed. McGraw-Hill, New York. Inagaki, Hamm, Arakawa, Painter (BIB10) 1974; 61 10.1016/j.bios.2003.08.021_BIB19 10.1016/j.bios.2003.08.021_BIB16 Wyatt (10.1016/j.bios.2003.08.021_BIB32) 1972; 37 10.1016/j.bios.2003.08.021_BIB15 10.1016/j.bios.2003.08.021_BIB12 Koch (10.1016/j.bios.2003.08.021_BIB13) 1968; 18 Mattley (10.1016/j.bios.2003.08.021_BIB17) 2000; 71 Thormälen (10.1016/j.bios.2003.08.021_BIB25) 1985; 14 Latimer (10.1016/j.bios.2003.08.021_BIB14) 1977; 63 10.1016/j.bios.2003.08.021_BIB11 10.1016/j.bios.2003.08.021_BIB30 Wyatt (10.1016/j.bios.2003.08.021_BIB31) 1968; 7 10.1016/j.bios.2003.08.021_BIB1 10.1016/j.bios.2003.08.021_BIB2 10.1016/j.bios.2003.08.021_BIB28 10.1016/j.bios.2003.08.021_BIB27 Warth (10.1016/j.bios.2003.08.021_BIB29) 1983; 130 10.1016/j.bios.2003.08.021_BIB5 10.1016/j.bios.2003.08.021_BIB6 10.1016/j.bios.2003.08.021_BIB3 Inagaki (10.1016/j.bios.2003.08.021_BIB10) 1974; 61 10.1016/j.bios.2003.08.021_BIB24 10.1016/j.bios.2003.08.021_BIB4 10.1016/j.bios.2003.08.021_BIB9 10.1016/j.bios.2003.08.021_BIB7 10.1016/j.bios.2003.08.021_BIB8 Purcell (10.1016/j.bios.2003.08.021_BIB21) 1969; 158 Mourant (10.1016/j.bios.2003.08.021_BIB18) 2000; 5 10.1016/j.bios.2003.08.021_BIB22 10.1016/j.bios.2003.08.021_BIB20 Ross (10.1016/j.bios.2003.08.021_BIB23) 1957; 16 Tuminello (10.1016/j.bios.2003.08.021_BIB26) 1997; 36 |
References_xml | – volume: 18 start-page: 133 year: 1968 end-page: 156 ident: BIB13 article-title: Theory of the angular dependence of light Scattered by bacteria and similar-sized biological objects publication-title: J. Theoret. Biol. contributor: fullname: Koch – volume: 7 start-page: 1879 year: 1968 end-page: 1896 ident: BIB31 article-title: Differential light scattering: a physical method for identifying living bacterial cells publication-title: Appl. Opt. contributor: fullname: Wyatt – volume: 63 start-page: 310 year: 1977 end-page: 316 ident: BIB14 article-title: Scattering by ellipsoids of revolution; a comparison of theoretical methods publication-title: J. Colloid Interface Sci. contributor: fullname: Barber – volume: 16 start-page: 418 year: 1957 end-page: 425 ident: BIB23 article-title: The water and solid content of bacterial spores and vegetative cells as indicated by refractive index measurements publication-title: J. Gen. Microbiol. contributor: fullname: Billing – volume: 36 start-page: 2818 year: 1997 end-page: 2823 ident: BIB26 article-title: Optical properties of publication-title: Appl. Opt. contributor: fullname: Milham – volume: 37 start-page: 493 year: 1972 end-page: 501 ident: BIB32 article-title: Structure of single bacteria from light scattering publication-title: J. Theor. Biol. contributor: fullname: Phillips – volume: 14 start-page: 933 year: 1985 end-page: 945 ident: BIB25 article-title: Refractive index of water and its dependence on wavelength, temperature, and density publication-title: J. Phys. Chem. Ref. Data contributor: fullname: Grigull – volume: 71 start-page: 610 year: 2000 end-page: 619 ident: BIB17 article-title: Light scattering and absorption model for the quantitative interpretation of human blood-platelet spectral data publication-title: Photochem. Photobiol. contributor: fullname: Garcia-Rubio – volume: 158 start-page: 433 year: 1969 end-page: 440 ident: BIB21 article-title: On the absorption and emission of light by interstellar grains publication-title: Astrophys. J. contributor: fullname: Purcell – volume: 5 start-page: 131 year: 2000 end-page: 137 ident: BIB18 article-title: Light scattering from cells: the contribution of the nucleus and the effects of proliferative status publication-title: J. Biomed. Opt. contributor: fullname: Freyer – volume: 61 start-page: 4246 year: 1974 end-page: 4250 ident: BIB10 article-title: Optical and dielectric properties of DNA in the extreme ultraviolet publication-title: J. Chem. Phys. contributor: fullname: Painter – volume: 130 start-page: 502 year: 1983 end-page: 505 ident: BIB29 article-title: Determination of dipicolinic acid in bacterial spores by derivative spectroscopy publication-title: Anal. Biochem. contributor: fullname: Warth – ident: 10.1016/j.bios.2003.08.021_BIB4 – volume: 16 start-page: 418 year: 1957 ident: 10.1016/j.bios.2003.08.021_BIB23 article-title: The water and solid content of bacterial spores and vegetative cells as indicated by refractive index measurements publication-title: J. Gen. Microbiol. doi: 10.1099/00221287-16-2-418 contributor: fullname: Ross – ident: 10.1016/j.bios.2003.08.021_BIB28 – ident: 10.1016/j.bios.2003.08.021_BIB12 doi: 10.1016/B978-0-12-404550-7.50008-7 – ident: 10.1016/j.bios.2003.08.021_BIB30 – ident: 10.1016/j.bios.2003.08.021_BIB27 doi: 10.1063/1.3060205 – volume: 130 start-page: 502 year: 1983 ident: 10.1016/j.bios.2003.08.021_BIB29 article-title: Determination of dipicolinic acid in bacterial spores by derivative spectroscopy publication-title: Anal. Biochem. doi: 10.1016/0003-2697(83)90624-3 contributor: fullname: Warth – ident: 10.1016/j.bios.2003.08.021_BIB24 – ident: 10.1016/j.bios.2003.08.021_BIB22 – volume: 5 start-page: 131 issue: 2 year: 2000 ident: 10.1016/j.bios.2003.08.021_BIB18 article-title: Light scattering from cells: the contribution of the nucleus and the effects of proliferative status publication-title: J. Biomed. Opt. doi: 10.1117/1.429979 contributor: fullname: Mourant – volume: 61 start-page: 4246 year: 1974 ident: 10.1016/j.bios.2003.08.021_BIB10 article-title: Optical and dielectric properties of DNA in the extreme ultraviolet publication-title: J. Chem. Phys. doi: 10.1063/1.1681724 contributor: fullname: Inagaki – volume: 7 start-page: 1879 year: 1968 ident: 10.1016/j.bios.2003.08.021_BIB31 article-title: Differential light scattering: a physical method for identifying living bacterial cells publication-title: Appl. Opt. doi: 10.1364/AO.7.001879 contributor: fullname: Wyatt – volume: 36 start-page: 2818 issue: 13 year: 1997 ident: 10.1016/j.bios.2003.08.021_BIB26 article-title: Optical properties of Bacillus subtilis from 0.2 to 2.5 μm publication-title: Appl. Opt. doi: 10.1364/AO.36.002818 contributor: fullname: Tuminello – ident: 10.1016/j.bios.2003.08.021_BIB2 – ident: 10.1016/j.bios.2003.08.021_BIB8 – ident: 10.1016/j.bios.2003.08.021_BIB6 – volume: 63 start-page: 310 issue: 2 year: 1977 ident: 10.1016/j.bios.2003.08.021_BIB14 article-title: Scattering by ellipsoids of revolution; a comparison of theoretical methods publication-title: J. Colloid Interface Sci. doi: 10.1016/0021-9797(78)90140-6 contributor: fullname: Latimer – ident: 10.1016/j.bios.2003.08.021_BIB3 – volume: 18 start-page: 133 year: 1968 ident: 10.1016/j.bios.2003.08.021_BIB13 article-title: Theory of the angular dependence of light Scattered by bacteria and similar-sized biological objects publication-title: J. Theoret. Biol. doi: 10.1016/0022-5193(68)90174-4 contributor: fullname: Koch – volume: 71 start-page: 610 issue: 5 year: 2000 ident: 10.1016/j.bios.2003.08.021_BIB17 article-title: Light scattering and absorption model for the quantitative interpretation of human blood-platelet spectral data publication-title: Photochem. Photobiol. doi: 10.1562/0031-8655(2000)071<0610:LSAAMF>2.0.CO;2 contributor: fullname: Mattley – ident: 10.1016/j.bios.2003.08.021_BIB16 doi: 10.1117/12.418743 – volume: 37 start-page: 493 year: 1972 ident: 10.1016/j.bios.2003.08.021_BIB32 article-title: Structure of single bacteria from light scattering publication-title: J. Theor. Biol. doi: 10.1016/0022-5193(72)90087-2 contributor: fullname: Wyatt – volume: 14 start-page: 933 year: 1985 ident: 10.1016/j.bios.2003.08.021_BIB25 article-title: Refractive index of water and its dependence on wavelength, temperature, and density publication-title: J. Phys. Chem. Ref. Data doi: 10.1063/1.555743 contributor: fullname: Thormälen – ident: 10.1016/j.bios.2003.08.021_BIB15 – volume: 158 start-page: 433 year: 1969 ident: 10.1016/j.bios.2003.08.021_BIB21 article-title: On the absorption and emission of light by interstellar grains publication-title: Astrophys. J. doi: 10.1086/150207 contributor: fullname: Purcell – ident: 10.1016/j.bios.2003.08.021_BIB20 – ident: 10.1016/j.bios.2003.08.021_BIB9 – ident: 10.1016/j.bios.2003.08.021_BIB11 – ident: 10.1016/j.bios.2003.08.021_BIB1 – ident: 10.1016/j.bios.2003.08.021_BIB19 – ident: 10.1016/j.bios.2003.08.021_BIB5 – ident: 10.1016/j.bios.2003.08.021_BIB7 |
SSID | ssj0007190 |
Score | 2.029028 |
Snippet | Multiwavelength ultraviolet/visible (UV-Vis) spectra of microorganisms and cell suspensions contain quantitative information on properties such as number,... |
SourceID | proquest crossref pubmed pascalfrancis elsevier |
SourceType | Aggregation Database Index Database Publisher |
StartPage | 893 |
SubjectTerms | Absorbance Algorithms Bacillus globigii Biological and medical sciences Biosensor Biosensors Biotechnology Escherichia coli - chemistry Escherichia coli - isolation & purification Feasibility Studies Fundamental and applied biological sciences. Psychology Methods. Procedures. Technologies Microorganisms Models, Biological Multiwavelength spectroscopy Optical properties Reproducibility of Results Scattering Sensitivity and Specificity Spectrum Analysis - methods Spores, Bacterial - chemistry Spores, Bacterial - isolation & purification Statistics as Topic Various methods and equipments |
Title | Quantitative spectroscopy analysis of prokaryotic cells: vegetative cells and spores |
URI | https://dx.doi.org/10.1016/j.bios.2003.08.021 https://www.ncbi.nlm.nih.gov/pubmed/15128109 https://search.proquest.com/docview/17826251 https://search.proquest.com/docview/71912934 |
Volume | 19 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1LT9wwEB7BIiQQQpTSsjy2PnBD6caJnTjcEAJtWxWpAiRukeM4aFspWbG7lfbS385M7BQ4LAeOsfzS2Jn5xvZ8A3ASZyYrUenRpTs6KCqNA40-WKCUKHloZRZaikb-eZ2M7sT3e3m_AhddLAw9q_S63-n0Vlv7kqGX5nAyHg9viEIPwQjqYNqpRCi5huZIiB6snX_7Mbr-r5BT7o5aiHKPGvjYGffMqxg3LWt33DJ5RnyZfdqa6ClKrXLpLpbj0dYuXe3AtgeU7NzN-QOs2HoX1l2KycUubL4gHPwIt7_mum7DylDJsTbKktgsm8mCac9OwpqK4aB_9OOiwS4ZnexPz9hf-2B9s7YE65eMXGI73YO7q8vbi1Hg8yoEBk3WLKhUWqZKigpdu9SEuhJo1vHTSgQbxB5jlUW_sDBCJzoxRZxVkTAGsU9UmKhM40_Qq5va7gNLLNaoCpGEyoq4VOgPRVUmjJSWJ1aYPpx20swnjj4j796V_c5J9pQHM84pFWbE-yA7geevNkGO-v3NdoNXq_M8lBQpIkTVhy_dcuX4-5CcdG2b-TTniJDQBeTLa-DuIUwk-vDZrfOL3ukaMswO3jntQ9hw74DigMsj6M0e5_YYIc6sGMDq13984DfyE7bZ-cM |
link.rule.ids | 315,786,790,4521,24144,27957,27958,45620,45714 |
linkProvider | Elsevier |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1La9wwEB7ChtKWUtL0tc1Lh9yKWcuWbLm3EBo2TbJQsoHchCzLYVuwl-xuYf99Zyy5SQ7bQ44WejGSR99IM98AHKeFLSpUevTojgaKytPIoA0WKSUqHjtZxI6ika8m2fhG_LiVt1tw2sfCkFtl0P1ep3faOpSMgjRH89lsdE0UeghGUAfTTiVCyW0hcy4GsH1yfjGe_FPIOfdXLUS5Rw1C7Ix38ypnbcfanXZMngnfdD69mZsFSq326S4249HuXDrbgbcBULITP-d3sOWaXXjhU0yud-H1I8LB9zD9uTJNF1aGSo51UZbEZtnO18wEdhLW1gwH_W3u1y12yehmf_GN_XF3LjTrSrB-xcgkdosPcHP2fXo6jkJehcjikbWMapVXuZKiRtMut7GpBR7r-Okkgg1ij3HKoV1YWmEyk9kyLepEWIvYJyltUuXpRxg0beM-A8sc1qhLkcXKibRSaA8ldSGslI5nTtghfO2lqeeePkP3fmW_NMme8mCmmlJhJnwIshe4frIJNOr3_7Y7fLI6D0NJkSNCVEM46pdL4-9DcjKNa1cLzREhoQnIN9fA3UOYSAzhk1_nR73TM2RcfHnmtI_g5Xh6dakvzycXe_DK-wSlEZf7MFjer9wBwp1leRi281-7yPu1 |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Quantitative+spectroscopy+analysis+of+prokaryotic+cells%3A+vegetative+cells+and+spores&rft.jtitle=Biosensors+%26+bioelectronics&rft.au=Alupoaei%2C+Catalina+E&rft.au=Olivares%2C+Jose+A&rft.au=Garc%C4%B1%CC%81a-Rubio%2C+Luis+H&rft.date=2004-03-15&rft.pub=Elsevier+B.V&rft.issn=0956-5663&rft.eissn=1873-4235&rft.volume=19&rft.issue=8&rft.spage=893&rft.epage=903&rft_id=info:doi/10.1016%2Fj.bios.2003.08.021&rft.externalDocID=S0956566303003002 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0956-5663&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0956-5663&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0956-5663&client=summon |