Single-molecule characterization of SV40 replisome and novel factors: human FPC and Mcm10

• Published in: Nucleic acids research Vol. 52; no. 15; pp. 8880 - 8896
• Main Authors: Ouyang, Yujing, Al-Amodi, Amani, Tehseen, Muhammad, Alhudhali, Lubna, Shirbini, Afnan, Takahashi, Masateru, Raducanu, Vlad-Stefan, Yi, Gang, Danazumi, Ammar Usman, De Biasio, Alfredo, Hamdan, Samir M
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 27.08.2024
• Subjects: Antigens, Polyomavirus Transforming - genetics; Antigens, Polyomavirus Transforming - metabolism; DNA Helicases - genetics; DNA Helicases - metabolism; DNA Polymerase III - genetics; DNA Polymerase III - metabolism; DNA Replication; DNA, Single-Stranded - metabolism; DNA, Viral - genetics; DNA, Viral - metabolism; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; DNA-Directed DNA Polymerase; Genome Integrity, Repair and; Humans; Minichromosome Maintenance Proteins - genetics; Minichromosome Maintenance Proteins - metabolism; Multienzyme Complexes; Replication Protein A - metabolism; Simian virus 40 - genetics; Simian virus 40 - metabolism; Single Molecule Imaging; Virus Replication

The simian virus 40 (SV40) replisome only encodes for its helicase; large T-antigen (L-Tag), while relying on the host for the remaining proteins, making it an intriguing model system. Despite being one of the earliest reconstituted eukaryotic systems, the interactions coordinating its activities an...