Unleashing the Influence of cAMP Receptor Protein: The Master Switch of Bacteriocin Export in Pectobacterium carotovorum subsp. carotovorum

Pectobacterium carotovorum subsp. carotovorum (Pcc) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that can kill related strains in response to factors in the environment such as UV exposure or nutritional deficiency. The function of the catabo...

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Published inInternational journal of molecular sciences Vol. 24; no. 11; p. 9752
Main Authors Chang, Chung-Pei, Lagitnay, Ruchi Briam James Sersenia, Li, Tzu-Rong, Lai, Wei-Ting, Derilo, Reymund Calanga, Chuang, Duen-Yau
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 05.06.2023
MDPI
Subjects
Bacteria
Bacterial infections
E coli
Genes
Metabolism
Polymerase chain reaction
Proteins
Virulence
flagellar-type III secretion
Pectobacterium carotovorum subsp. carotovorum
low-molecular-weight bacteriocin
cAMP receptor protein
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Abstract Pectobacterium carotovorum subsp. carotovorum (Pcc) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that can kill related strains in response to factors in the environment such as UV exposure or nutritional deficiency. The function of the catabolite activator protein (CAP), also known as the cyclic AMP receptor protein (CRP), as a regulator of carocin synthesis was examined. The crp gene was knocked out as part of the investigation, and the outcomes were assessed both in vivo and in vitro. Analysis of the DNA sequence upstream of the translation initiation site of carocin S3 revealed two putative binding sites for CRP that were confirmed using a biotinylated probe pull-down experiment. This study revealed that the deletion of crp inhibited genes involved in extracellular bacteriocin export via the flagellar type III secretion system and impacted the production of many low-molecular-weight bacteriocins. The biotinylated probe pull-down test demonstrated that when UV induction was missing, CRP preferentially attached to one of the two CAP sites while binding to both when UV induction was present. In conclusion, our research aimed to simulate the signal transduction system that controls the expression of the carocin gene in response to UV induction.
AbstractList Pectobacterium carotovorum subsp. carotovorum (Pcc) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that can kill related strains in response to factors in the environment such as UV exposure or nutritional deficiency. The function of the catabolite activator protein (CAP), also known as the cyclic AMP receptor protein (CRP), as a regulator of carocin synthesis was examined. The crp gene was knocked out as part of the investigation, and the outcomes were assessed both in vivo and in vitro. Analysis of the DNA sequence upstream of the translation initiation site of carocin S3 revealed two putative binding sites for CRP that were confirmed using a biotinylated probe pull-down experiment. This study revealed that the deletion of crp inhibited genes involved in extracellular bacteriocin export via the flagellar type III secretion system and impacted the production of many low-molecular-weight bacteriocins. The biotinylated probe pull-down test demonstrated that when UV induction was missing, CRP preferentially attached to one of the two CAP sites while binding to both when UV induction was present. In conclusion, our research aimed to simulate the signal transduction system that controls the expression of the carocin gene in response to UV induction.
( ) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that can kill related strains in response to factors in the environment such as UV exposure or nutritional deficiency. The function of the catabolite activator protein (CAP), also known as the cyclic AMP receptor protein (CRP), as a regulator of carocin synthesis was examined. The gene was knocked out as part of the investigation, and the outcomes were assessed both in vivo and in vitro. Analysis of the DNA sequence upstream of the translation initiation site of carocin S3 revealed two putative binding sites for CRP that were confirmed using a biotinylated probe pull-down experiment. This study revealed that the deletion of inhibited genes involved in extracellular bacteriocin export via the flagellar type III secretion system and impacted the production of many low-molecular-weight bacteriocins. The biotinylated probe pull-down test demonstrated that when UV induction was missing, CRP preferentially attached to one of the two CAP sites while binding to both when UV induction was present. In conclusion, our research aimed to simulate the signal transduction system that controls the expression of the carocin gene in response to UV induction.
Pectobacterium carotovorum subsp. carotovorum (Pcc) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that can kill related strains in response to factors in the environment such as UV exposure or nutritional deficiency. The function of the catabolite activator protein (CAP), also known as the cyclic AMP receptor protein (CRP), as a regulator of carocin synthesis was examined. The crp gene was knocked out as part of the investigation, and the outcomes were assessed both in vivo and in vitro. Analysis of the DNA sequence upstream of the translation initiation site of carocin S3 revealed two putative binding sites for CRP that were confirmed using a biotinylated probe pull-down experiment. This study revealed that the deletion of crp inhibited genes involved in extracellular bacteriocin export via the flagellar type III secretion system and impacted the production of many low-molecular-weight bacteriocins. The biotinylated probe pull-down test demonstrated that when UV induction was missing, CRP preferentially attached to one of the two CAP sites while binding to both when UV induction was present. In conclusion, our research aimed to simulate the signal transduction system that controls the expression of the carocin gene in response to UV induction.Pectobacterium carotovorum subsp. carotovorum (Pcc) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that can kill related strains in response to factors in the environment such as UV exposure or nutritional deficiency. The function of the catabolite activator protein (CAP), also known as the cyclic AMP receptor protein (CRP), as a regulator of carocin synthesis was examined. The crp gene was knocked out as part of the investigation, and the outcomes were assessed both in vivo and in vitro. Analysis of the DNA sequence upstream of the translation initiation site of carocin S3 revealed two putative binding sites for CRP that were confirmed using a biotinylated probe pull-down experiment. This study revealed that the deletion of crp inhibited genes involved in extracellular bacteriocin export via the flagellar type III secretion system and impacted the production of many low-molecular-weight bacteriocins. The biotinylated probe pull-down test demonstrated that when UV induction was missing, CRP preferentially attached to one of the two CAP sites while binding to both when UV induction was present. In conclusion, our research aimed to simulate the signal transduction system that controls the expression of the carocin gene in response to UV induction.
Pectobacterium carotovorum subsp. carotovorum ( Pcc ) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that can kill related strains in response to factors in the environment such as UV exposure or nutritional deficiency. The function of the catabolite activator protein (CAP), also known as the cyclic AMP receptor protein (CRP), as a regulator of carocin synthesis was examined. The crp gene was knocked out as part of the investigation, and the outcomes were assessed both in vivo and in vitro. Analysis of the DNA sequence upstream of the translation initiation site of carocin S3 revealed two putative binding sites for CRP that were confirmed using a biotinylated probe pull-down experiment. This study revealed that the deletion of crp inhibited genes involved in extracellular bacteriocin export via the flagellar type III secretion system and impacted the production of many low-molecular-weight bacteriocins. The biotinylated probe pull-down test demonstrated that when UV induction was missing, CRP preferentially attached to one of the two CAP sites while binding to both when UV induction was present. In conclusion, our research aimed to simulate the signal transduction system that controls the expression of the carocin gene in response to UV induction.
Author Lagitnay, Ruchi Briam James Sersenia
Chang, Chung-Pei
Lai, Wei-Ting
Derilo, Reymund Calanga
Chuang, Duen-Yau
Li, Tzu-Rong
AuthorAffiliation 3 Department of Chemistry, National Chung Hsing University, Taichung City 400, Taiwan; yamyrose@gmail.com (T.-R.L.); pppolly425@gmail.com (W.-T.L.); rcderilo@nvsu.edu.ph (R.C.D.)
2 College of Arts and Sciences, Bayombong Campus, Nueva Vizcaya State University, Bayombong 3700, Philippines; rslagitnay@nvsu.edu.ph
1 Department of Anesthesiology, Show Chwan Memorial Hospital, Changhua 500, Taiwan; fentanylpei@yahoo.com.tw
4 College of Teacher Education, Bambang Campus, Nueva Vizcaya State University, Bambang 3702, Philippines
AuthorAffiliation_xml – name: 1 Department of Anesthesiology, Show Chwan Memorial Hospital, Changhua 500, Taiwan; fentanylpei@yahoo.com.tw
– name: 2 College of Arts and Sciences, Bayombong Campus, Nueva Vizcaya State University, Bayombong 3700, Philippines; rslagitnay@nvsu.edu.ph
– name: 4 College of Teacher Education, Bambang Campus, Nueva Vizcaya State University, Bambang 3702, Philippines
– name: 3 Department of Chemistry, National Chung Hsing University, Taichung City 400, Taiwan; yamyrose@gmail.com (T.-R.L.); pppolly425@gmail.com (W.-T.L.); rcderilo@nvsu.edu.ph (R.C.D.)
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Issue 11
Keywords flagellar-type III secretion
Pectobacterium carotovorum subsp. carotovorum
low-molecular-weight bacteriocin
cAMP receptor protein
Language English
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These authors contributed equally to this work.
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Snippet Pectobacterium carotovorum subsp. carotovorum (Pcc) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that...
( ) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin that can kill related strains in response to factors...
Pectobacterium carotovorum subsp. carotovorum ( Pcc ) is a Gram-negative phytopathogenic bacterium that produces carocin, a low-molecular-weight bacteriocin...
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StartPage 9752
SubjectTerms Bacteria
Bacterial infections
E coli
Genes
Metabolism
Polymerase chain reaction
Proteins
Virulence
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Title Unleashing the Influence of cAMP Receptor Protein: The Master Switch of Bacteriocin Export in Pectobacterium carotovorum subsp. carotovorum
URI https://www.ncbi.nlm.nih.gov/pubmed/37298703
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Volume 24
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