Selection of reference genes for real-time expression studies in Streptococcus agalactiae

• Published in: Journal of microbiological methods Vol. 90; no. 3; pp. 220 - 227
• Main Authors: Florindo, C., Ferreira, R., Borges, V., Spellerberg, B., Gomes, J.P., Borrego, M.J.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.09.2012; Elsevier
• Subjects: bacteria; bacterial infections; Bacteriological methods and techniques used in bacteriology; Bacteriology; bioinformatics; Biological and medical sciences; Calibration; DNA; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene Expression Regulation, Bacterial; genes; Genes, Bacterial; messenger RNA; Microbiology; neonates; Normalization; quantitative polymerase chain reaction; Real-Time Polymerase Chain Reaction - standards; Reference genes; Reference Standards; Reproducibility of Results; reverse transcriptase polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - standards; RNA, Bacterial - genetics; RNA, Bacterial - isolation & purification; Streptococcus agalactiae; Streptococcus agalactiae - genetics; Streptococcus agalactiae - growth & development; temperature; Transcriptome; virulence; Normalization; Streptococcus agalactiae; Gene expression; Reference genes; Streptococcaceae; Gene; Bacteria; Micrococcales; Real time

Streptococcus agalactiae, group B streptococci (GBS) is the leading cause of severe bacterial infections in newborns. GBS expression studies allowed the identification and characterization of virulence factors and a better understanding of the host–pathogen–environment interactions. The measurement...