A Rapid Nanofocusing Method for a Deep-Sea Gene Sequencing Microscope Based on Critical Illumination

• Published in: Sensors (Basel, Switzerland) Vol. 24; no. 15; p. 5010
• Main Authors: Gao, Ming, Shu, Fengfeng, Zhou, Wenchao, Li, Huan, Wu, Yihui, Wang, Yue, Zhao, Shixun, Song, Zihan
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.08.2024
• Subjects: Accuracy; Algorithms; auto-focus; critical illumination; deep sea; edge gradient; gene sequencing; Genes; Imaging systems; Lasers; Lighting - instrumentation; Methods; Microscopy - instrumentation; Microscopy - methods; Salinity; Sequence Analysis, DNA - instrumentation; Sequence Analysis, DNA - methods; China; auto-focus; critical illumination; edge gradient; gene sequencing; deep sea

In the deep-sea environment, the volume available for an in-situ gene sequencer is severely limited. In addition, optical imaging systems are subject to real-time, large-scale defocusing problems caused by ambient temperature fluctuations and vibrational perturbations. To address these challenges, w...