High-performance liquid chromatographic assay of serum glycated albumin

• Published in: Diabetologia Vol. 31; no. 8; p. 627
• Main Authors: Shima, K, Ito, N, Abe, F, Hirota, M, Yano, M, Yamamoto, Y, Uchida, T, Noguchi, K
• Format: Journal Article
• Language: English
• Published: Germany 01.08.1988
• Subjects: Biomarkers - blood; Blood Glucose - metabolism; Blood Proteins - metabolism; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Circadian Rhythm; Diabetes Mellitus, Type 1 - blood; Diabetes Mellitus, Type 2 - blood; Glycosylation; Humans; Reference Values; Serum Albumin - analysis; Serum Albumin - metabolism

A method for determination of serum glycated albumin by high-performance liquid chromatography is presented. The system involves anion exchange chromatography to separate albumin and consecutive boronate affinity chromatography to separate glycated and nonglycated albumin. The method is rapid (20 mi...