Isolation and escape mapping of broadly neutralizing antibodies against emerging delta-coronaviruses

• Published in: Immunity (Cambridge, Mass.) Vol. 57; no. 12; pp. 2914 - 2927.e7
• Main Authors: Rexhepaj, Megi, Asarnow, Daniel, Perruzza, Lisa, Park, Young-Jun, Guarino, Barbara, Mccallum, Mathew, Culap, Katja, Saliba, Christian, Leoni, Giada, Balmelli, Alessio, Yoshiyama, Courtney N., Dickinson, Miles S., Quispe, Joel, Brown, Jack T., Tortorici, M. Alejandra, Sprouse, Kaitlin R., Taylor, Ashley L., Corti, Davide, Starr, Tyler N., Benigni, Fabio, Veesler, David
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 10.12.2024
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Broadly Neutralizing Antibodies - immunology; cryo-EM structures; Cryoelectron Microscopy; deep mutational scanning; Epitope Mapping - methods; Epitopes - immunology; Humans; Mice; neutralizing antibodies; PDCoV; porcine deltacoronavirus; spike glycoprotein; Spike Glycoprotein, Coronavirus - chemistry; Spike Glycoprotein, Coronavirus - immunology; zoonosis; porcine deltacoronavirus; PDCoV; spike glycoprotein; deep mutational scanning; neutralizing antibodies; zoonosis; cryo-EM structures

Porcine delta-coronavirus (PDCoV) spillovers were recently detected in febrile children, underscoring the recurrent zoonoses of divergent CoVs. To date, no vaccines or specific therapeutics are approved for use in humans against PDCoV. To prepare for possible future PDCoV epidemics, we isolated PDCo...