Development of a new LC–MS/MS based enzyme activity assay for recombinant urate oxidase in plasma and its application to pharmacokinetics in human - Luo, Xi; Cai, Ning Fang; Cheng, Ze Neng (Journal of pharmaceutical and biomedical analysis, 2013)
Keywords: 15N-allantoin; Calibration; China; Chromatography, Liquid - methods; correlation; enzymatic reactions; enzyme activity; Escherichia coli; Escherichia coli - metabolism; Female; formic acid; Humans; Infusions, Intravenous; intravenous injection; ionization; LC–MS/MS; liquid chromatography; Male; methanol; monitoring; Pharmacokinetics; Recombinant Proteins - blood; Recombinant urate oxidase; Reproducibility of Results; tandem mass spectrometry; Tandem Mass Spectrometry - methods; Urate Oxidase - blood; Urate Oxidase - isolation & purification; volunteers; Young Adult; China; 15N-allantoin; Pharmacokinetics; Recombinant urate oxidase; LC–MS/MS