CRISPR-Cas12a exhibits metal-dependent specificity switching

• Published in: Nucleic acids research Vol. 52; no. 16; pp. 9343 - 9359
• Main Authors: Nguyen, Giang T, Schelling, Michael A, Raju, Akshara, Buscher, Kathryn A, Sritharan, Aneisha, Sashital, Dipali G
• Format: Journal Article
• Language: English
• Published: England Oxford University Press 09.09.2024
• Subjects: NAR Breakthrough

Cas12a is the immune effector of type V-A CRISPR-Cas systems and has been co-opted for genome editing and other biotechnology tools. The specificity of Cas12a has been the subject of extensive investigation both in vitro and in genome editing experiments. However, in vitro studies have often been pe...