Lipoplex‐Functionalized Thin‐Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation
A gene‐activated surface coating is presented as a strategy to design smart biomaterials for bone tissue engineering. The thin‐film coating is based on polyelectrolyte multilayers composed of collagen I and chondroitin sulfate, two main biopolymers of the bone extracellular matrix, which are fabrica...
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Published in | Advanced healthcare materials Vol. 12; no. 5; pp. e2201978 - n/a |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
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01.02.2023
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Abstract | A gene‐activated surface coating is presented as a strategy to design smart biomaterials for bone tissue engineering. The thin‐film coating is based on polyelectrolyte multilayers composed of collagen I and chondroitin sulfate, two main biopolymers of the bone extracellular matrix, which are fabricated by layer‐by‐layer assembly. For further functionalization, DNA/lipid‐nanoparticles (lipoplexes) are incorporated into the multilayers. The polyelectrolyte multilayer fabrication and lipoplex deposition are analyzed by surface sensitive analytical methods that demonstrate successful thin‐film formation, fibrillar structuring of collagen, and homogenous embedding of lipoplexes. Culture of mesenchymal stem cells on the lipoplex functionalized multilayer results in excellent attachment and growth of them, and also, their ability to take up cargo like fluorescence‐labelled DNA from lipoplexes. The functionalization of the multilayer with lipoplexes encapsulating DNA encoding for transient expression of bone morphogenetic protein 2 induces osteogenic differentiation of mesenchymal stem cells, which is shown by mRNA quantification for osteogenic genes and histochemical staining. In summary, the novel gene‐functionalized and extracellular matrix mimicking multilayer composed of collagen I, chondroitin sulfate, and lipoplexes, represents a smart surface functionalization that holds great promise for tissue engineering constructs and implant coatings to promote regeneration of bone and other tissues.
An extracellular matrix‐inspired polyelectrolyte multilayer film is functionalized with lipoplexes and used as gene activated biomaterial surface coating for application in regenerative medicine. Collagen I‐chondroitin sulfate multilayers with embedded lipoplexes having plasmids encoding for bone morphogenic protein 2 gene promote adhesion and growth of mesenchymal stem cells and transfect them efficiently inducing their osteogenic differentiation. |
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AbstractList | A gene-activated surface coating is presented as a strategy to design smart biomaterials for bone tissue engineering. The thin-film coating is based on polyelectrolyte multilayers composed of collagen I and chondroitin sulfate, two main biopolymers of the bone extracellular matrix, which are fabricated by layer-by-layer assembly. For further functionalization, DNA/lipid-nanoparticles (lipoplexes) are incorporated into the multilayers. The polyelectrolyte multilayer fabrication and lipoplex deposition are analyzed by surface sensitive analytical methods that demonstrate successful thin-film formation, fibrillar structuring of collagen, and homogenous embedding of lipoplexes. Culture of mesenchymal stem cells on the lipoplex functionalized multilayer results in excellent attachment and growth of them, and also, their ability to take up cargo like fluorescence-labelled DNA from lipoplexes. The functionalization of the multilayer with lipoplexes encapsulating DNA encoding for transient expression of bone morphogenetic protein 2 induces osteogenic differentiation of mesenchymal stem cells, which is shown by mRNA quantification for osteogenic genes and histochemical staining. In summary, the novel gene-functionalized and extracellular matrix mimicking multilayer composed of collagen I, chondroitin sulfate, and lipoplexes, represents a smart surface functionalization that holds great promise for tissue engineering constructs and implant coatings to promote regeneration of bone and other tissues. A gene‐activated surface coating is presented as a strategy to design smart biomaterials for bone tissue engineering. The thin‐film coating is based on polyelectrolyte multilayers composed of collagen I and chondroitin sulfate, two main biopolymers of the bone extracellular matrix, which are fabricated by layer‐by‐layer assembly. For further functionalization, DNA/lipid‐nanoparticles (lipoplexes) are incorporated into the multilayers. The polyelectrolyte multilayer fabrication and lipoplex deposition are analyzed by surface sensitive analytical methods that demonstrate successful thin‐film formation, fibrillar structuring of collagen, and homogenous embedding of lipoplexes. Culture of mesenchymal stem cells on the lipoplex functionalized multilayer results in excellent attachment and growth of them, and also, their ability to take up cargo like fluorescence‐labelled DNA from lipoplexes. The functionalization of the multilayer with lipoplexes encapsulating DNA encoding for transient expression of bone morphogenetic protein 2 induces osteogenic differentiation of mesenchymal stem cells, which is shown by mRNA quantification for osteogenic genes and histochemical staining. In summary, the novel gene‐functionalized and extracellular matrix mimicking multilayer composed of collagen I, chondroitin sulfate, and lipoplexes, represents a smart surface functionalization that holds great promise for tissue engineering constructs and implant coatings to promote regeneration of bone and other tissues. An extracellular matrix‐inspired polyelectrolyte multilayer film is functionalized with lipoplexes and used as gene activated biomaterial surface coating for application in regenerative medicine. Collagen I‐chondroitin sulfate multilayers with embedded lipoplexes having plasmids encoding for bone morphogenic protein 2 gene promote adhesion and growth of mesenchymal stem cells and transfect them efficiently inducing their osteogenic differentiation. |
Author | Groth, Thomas Wölk, Christian Brito Barrera, Yazmin A. Husteden, Catharina Borges, João Langner, Andreas Mano, João F. Tegtmeyer, Sophia Schmelzer, Christian E. H. Giselbrecht, Julia Menzel, Matthias |
Author_xml | – sequence: 1 givenname: Catharina surname: Husteden fullname: Husteden, Catharina organization: Martin Luther University Halle‐Wittenberg – sequence: 2 givenname: Yazmin A. surname: Brito Barrera fullname: Brito Barrera, Yazmin A. organization: Martin Luther University Halle‐Wittenberg – sequence: 3 givenname: Sophia surname: Tegtmeyer fullname: Tegtmeyer, Sophia organization: Martin Luther University Halle‐Wittenberg – sequence: 4 givenname: João orcidid: 0000-0003-0126-8482 surname: Borges fullname: Borges, João organization: University of Aveiro – sequence: 5 givenname: Julia surname: Giselbrecht fullname: Giselbrecht, Julia organization: Martin Luther University Halle‐Wittenberg – sequence: 6 givenname: Matthias orcidid: 0000-0003-2294-0573 surname: Menzel fullname: Menzel, Matthias organization: Fraunhofer Institute for Microstructure of Materials and Systems (IMWS) – sequence: 7 givenname: Andreas surname: Langner fullname: Langner, Andreas organization: Martin Luther University Halle‐Wittenberg – sequence: 8 givenname: João F. orcidid: 0000-0002-2342-3765 surname: Mano fullname: Mano, João F. organization: University of Aveiro – sequence: 9 givenname: Christian E. H. orcidid: 0000-0002-1180-0201 surname: Schmelzer fullname: Schmelzer, Christian E. H. organization: Fraunhofer Institute for Microstructure of Materials and Systems (IMWS) – sequence: 10 givenname: Christian orcidid: 0000-0002-8067-7307 surname: Wölk fullname: Wölk, Christian email: christian.woelk@medizin.uni-leipzig.de organization: Leipzig University – sequence: 11 givenname: Thomas orcidid: 0000-0001-6647-9657 surname: Groth fullname: Groth, Thomas email: thomas.groth@pharmazie.uni-halle.de organization: Martin Luther University Halle‐Wittenberg |
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Keywords | chondroitin sulfate osteogenic differentiation collagen I bone morphogenic protein 2 human adipose-derived mesenchymal stem cells polyelectrolyte multilayers lipoplexes |
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Snippet | A gene‐activated surface coating is presented as a strategy to design smart biomaterials for bone tissue engineering. The thin‐film coating is based on... A gene-activated surface coating is presented as a strategy to design smart biomaterials for bone tissue engineering. The thin-film coating is based on... |
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SubjectTerms | bone morphogenic protein 2 Cell Differentiation chondroitin sulfate Chondroitin Sulfates Collagen collagen I Collagen Type I - genetics DNA - metabolism Extracellular Matrix - metabolism Gene Transfer Techniques human adipose‐derived mesenchymal stem cells lipoplexes Osteogenesis osteogenic differentiation polyelectrolyte multilayers Polyelectrolytes |
Title | Lipoplex‐Functionalized Thin‐Film Surface Coating Based on Extracellular Matrix Components as Local Gene Delivery System to Control Osteogenic Stem Cell Differentiation |
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