Identification, cloning, and R-Loop mapping of the polyhedrin gene from the multicapsid nuclear polyhedrosis virus of Orgyia pseudotsugata

• Published in: Virology (New York, N.Y.) Vol. 121; no. 1; pp. 51 - 60
• Main Authors: Rohrmann, George F., Leisy, Douglas J., Chow, Kuan-Chih, Pearson, George D., Beaudreau, George S.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.1982
• Subjects: nuclear polyhedrosis virus

Polyadenylated RNA was isolated from Orgyia pseudotsugata larvae 8–10 days postinfection with the multicapsid nuclear polyhedrosis virus. This RNA was centrifuged through a sucrose gradient and fractions enriched for polyhedrin mRNA were identified by in vitro translation. Complementary DNA made to...