An extensively validated whole-cell biosensor for specific, sensitive and high-throughput detection of antibacterial inhibitors targeting cell-wall biosynthesis

• Published in: Journal of antimicrobial chemotherapy Vol. 78; no. 3; pp. 646 - 655
• Main Authors: Galarion, Luiza H, Mitchell, Jennifer K, Randall, Christopher P, O’Neill, Alex J
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 02.03.2023
• Subjects: Anti-Bacterial Agents - pharmacology; beta-Galactosidase - metabolism; Biosensing Techniques; High-Throughput Screening Assays; Original Research; Staphylococcus aureus - metabolism

Abstract Background Whole-cell biosensor strains are powerful tools for antibacterial drug discovery, in principle allowing the identification of inhibitors acting on specific, high-value target pathways. Whilst a variety of biosensors have been described for detecting cell-wall biosynthesis inhibit...