Leucyl-tRNA Synthetase Regulates Lactation and Cell Proliferation via mTOR Signaling in Dairy Cow Mammary Epithelial Cells

• Published in: International journal of molecular sciences Vol. 15; no. 4; pp. 5952 - 5969
• Main Authors: Wang, Lina, Lin, Ye, Bian, Yanjie, Liu, Lili, Shao, Li, Lin, Lin, Qu, Bo, Zhao, Feng, Gao, Xuejun, Li, Qingzhang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 09.04.2014; Molecular Diversity Preservation International (MDPI)
• Subjects: Animals; Caseins - biosynthesis; Caseins - genetics; Cattle; Cell growth; Cell Proliferation; Cell Survival; Cells, Cultured; Cyclin D1 - biosynthesis; Cyclin D1 - genetics; Enzymes; Epithelial Cells - metabolism; Female; Glucose Transporter Type 1 - biosynthesis; Glucose Transporter Type 1 - genetics; Lactation - metabolism; Leucine - metabolism; Leucine - pharmacology; Leucine-tRNA Ligase - biosynthesis; Leucine-tRNA Ligase - genetics; Mammary Glands, Animal - metabolism; Pregnancy; Ribosomal Protein S6 Kinases, 90-kDa - biosynthesis; Ribosomal Protein S6 Kinases, 90-kDa - genetics; RNA Interference; RNA, Messenger - biosynthesis; RNA, Small Interfering; Signal Transduction; Sterol Regulatory Element Binding Protein 1 - biosynthesis; Sterol Regulatory Element Binding Protein 1 - genetics; TOR Serine-Threonine Kinases - biosynthesis; TOR Serine-Threonine Kinases - metabolism; Transfer RNA

The role of LeuRS, an aminoacyl-tRNA synthetase, as an intracellular l-leucine sensor for the mTORC1 pathway has been the subject of much research recently. Despite this, the association between LeuRS and lactation in dairy cow mammary epithelial cells (DCMECs) remains unknown. In this study, we fou...