Assay of centromere function using a human artificial chromosome

• Published in: Chromosoma Vol. 107; no. 6-7; pp. 406 - 416
• Main Authors: Masumoto, H, Ikeno, M, Nakano, M, Okazaki, T, Grimes, B, Cooke, H, Suzuki, N
• Format: Journal Article
• Language: English
• Published: Austria Springer Nature B.V 01.12.1998
• Subjects: Artificial chromosomes; Autoantigens; Cells, Cultured; Centromere - physiology; Centromere Protein B; Chromosomal Proteins, Non-Histone - physiology; Chromosome Segregation; Chromosomes, Artificial, Yeast; Chromosomes, Human, Pair 21 - physiology; Cloning, Molecular; DNA-Binding Proteins; Genetics; Humans; In Situ Hybridization, Fluorescence; Kinetochores; Nuclear Proteins; Repetitive Sequences, Nucleic Acid; Spindle Apparatus; Telomere; Transfection

In order to define a functional human centromere sequence, an artificial chromosome was constructed as a reproducible DNA molecule. Mammalian telomere repeats and a selectable marker were introduced into yeast artificial chromosomes (YACs) containing alphoid DNA from the centromere region of human c...