Targeted Metagenomic Sequencing-based Approach Applied to 2146 Tissue and Body Fluid Samples in Routine Clinical Practice

• Published in: Clinical infectious diseases Vol. 75; no. 10; pp. 1800 - 1808
• Main Authors: Flurin, Laure, Wolf, Matthew J, Mutchler, Melissa M, Daniels, Matthew L, Wengenack, Nancy L, Patel, Robin
• Format: Journal Article
• Language: English
• Published: US Oxford University Press 14.11.2022
• Subjects: Body Fluids - chemistry; DNA, Bacterial - analysis; DNA, Bacterial - genetics; High-Throughput Nucleotide Sequencing; Humans; Major; Metagenomics; RNA, Ribosomal, 16S - genetics; Sequence Analysis, DNA; targeted metagenomics; clinical metagenomics; tissue and body fluids; 16S ribosomal RNA gene PCR

Abstract Background The yield of next-generation sequencing (NGS) added to a Sanger sequencing–based 16S ribosomal RNA (rRNA) gene polymerase chain reaction (PCR) assay was evaluated in clinical practice for diagnosis of bacterial infection. Methods PCR targeting the V1 to V3 regions of the 16S rRNA...