Use of laser capture microdissection to detect integrated HIV-1 DNA in macrophages and astrocytes from autopsy brain tissues

The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from a...

Full description

Saved in:
Bibliographic Details
Published inJournal of neurovirology Vol. 12; no. 2; pp. 146 - 152
Main Authors Churchill, Melissa J, Gorry, Paul R, Cowley, Daniel, Lal, Luxshimi, Sonza, Secondo, Purcell, Damian FJ, Thompson, Katherine A, Gabuzda, Dana, McArthur, Justin C, Pardo, Carlos A, Wesselingh, Steven L
Format Journal Article
LanguageEnglish
Published London Informa UK Ltd 01.04.2006
Taylor & Francis
Subjects
AIDS Dementia Complex - virology
Antibiotics. Antiinfectious agents. Antiparasitic agents
Antiviral agents
Astrocytes - virology
Biological and medical sciences
Brain - virology
Cell Nucleus - chemistry
DNA, Viral - analysis
HIV-1 - isolation & purification
HIV-1 - physiology
Human immunodeficiency virus 1
Human viral diseases
Humans
Infectious diseases
Lasers
Macrophages - virology
Medical sciences
Microdissection
Pharmacology. Drug treatments
Polymerase Chain Reaction
Proviruses - physiology
Viral diseases
Viral diseases of the lymphoid tissue and the blood. Aids
Viral diseases of the nervous system
Virus Integration
HIV-1
Nervous system diseases
HIV-1 virus
Neuroglia
Central nervous system
Retroviridae
Astrocyte
Lentivirus
Capture
Provirus
HIVD
Encephalon
Infection
Virus
HIVE
Autopsy
Viral disease
Laser
Human immunodeficiency virus
Macrophage
Online AccessGet full text

Cover

More Information
Summary:The importance of astrocytes as a reservoir of human immunodeficiency virus type 1 (HIV-1) in the brain remains elusive. By combining immunohistochemistry, laser capture microdissection, and triple-nested Alu-PCR, we demonstrate integrated HIV-1 in astrocytes and macrophages isolated directly from autopsy brain tissues of HIV-1-infected subjects. The ability of HIV-1 to integrate in terminally differentiated astrocytes suggests a permanent reservoir of provirus in brain that will impact the development and likely success of strategies aimed at eradicating HIV-1.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:1355-0284
1538-2443
DOI:10.1080/13550280600748946