Enzymatic chemiluminescent assay of glucose by sequential-injection analysis with soluble enzyme and on-line sample dilution

• Published in: Analytica chimica acta Vol. 572; no. 1; pp. 140 - 147
• Main Authors: Economou, Anastasios, Panoutsou, Polyxeni, Themelis, Demetrius G.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 14.07.2006; Elsevier
• Subjects: Analytical chemistry; Chemical and thermal methods; Chemiluminescence; Chemistry; Exact sciences and technology; General, instrumentation; Glucose; Glucose oxidase; On-line sample dilution; Sequential-injection analysis; Glucose oxidase; Chemiluminescence; Glucose; Sequential-injection analysis; On-line sample dilution; Chemical analysis; Hydrogen peroxide; Instrumentation; Stopped flow method; Reversed flow; Sequential method; Cobalt II; Detection limit; Luminol; Sampling; Measurement error; Variation coefficient; Catalytic reaction; Enzyme; Automatic processing; Honey; Enzymatic method; Dilution; Sample cell; Relative error; Oxidoreductases; Catalyst; Enzymatic reaction

This work reports a sequential-injection analysis (SIA) method for the enzymatic assay of glucose with soluble glucose oxidase (GOD) and on-line sample dilution with chemiluminescence (CL) detection. A zone of sample was aspirated in the holding coil of the SIA manifold and, if necessary, was dilute...