Sequence and fine specificity analysis of primary 511 anti- phosphorylcholine antibodies

• Published in: The Journal of immunology (1950) Vol. 143; no. 12; pp. 4061 - 4068
• Main Authors: Feeney, AJ, Thuerauf, DJ
• Format: Journal Article
• Language: English
• Published: United States Am Assoc Immnol 15.12.1989
• Subjects: Amino Acid Sequence; Animals; Antibodies, Monoclonal - biosynthesis; Antibodies, Monoclonal - genetics; Antibodies, Monoclonal - isolation & purification; Antibody Affinity; Antibody Specificity; Base Sequence; Choline - analogs & derivatives; Haptens - immunology; Hemocyanins - immunology; Hybridomas - analysis; Immunoglobulin Heavy Chains - biosynthesis; Immunoglobulin Heavy Chains - genetics; Immunoglobulin Heavy Chains - isolation & purification; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Mice, Transgenic; Molecular Sequence Data; Phosphorylcholine - immunology

The primary antibody response of mice to phosphorylcholine (PC) is dominated by antibodies using the T15 L chain. Anti-PC antibodies using the 511 L chain are prominent only in secondary responses to PC coupled to proteins, are somatically mutated, and all have an extra amino acid at the Vh-D juncti...