Characterization of Virus-Inducible Orchid Argonaute 5b Promoter and Its Functional Characterization in Nicotiana benthamiana during Virus Infection

Plant ARGONAUTES (AGOs) play a significant role in the defense against viral infection. Previously, we have demonstrated that AGO5s encoded in Phalaenopsis aphrodite subsp. formosana (PaAGO5s) took an indispensable part in defense against major viruses. To understand the underlying defense mechanism...

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Published inInternational journal of molecular sciences Vol. 23; no. 17; p. 9825
Main Authors Kasi Viswanath, Kotapati, Kuo, Song-Yi, Huang, Ying-Wen, Tsao, Nai-Wen, Hu, Chung-Chi, Lin, Na-Sheng, Wang, Sheng-Yang, Hsu, Yau-Heiu
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 29.08.2022
MDPI
Subjects
Abscisic acid
Cloning
Gene expression
Infections
Nicotiana - genetics
Plants
Potexvirus - genetics
Proteins
Transcription Factors
Viral infections
Virus Diseases
Viruses
CymMV
NbMYB30
Argonautes 5b
ORSV
MeJA
SA
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Abstract Plant ARGONAUTES (AGOs) play a significant role in the defense against viral infection. Previously, we have demonstrated that AGO5s encoded in Phalaenopsis aphrodite subsp. formosana (PaAGO5s) took an indispensable part in defense against major viruses. To understand the underlying defense mechanism, we cloned PaAGO5s promoters (pPaAGO5s) and analyzed their activity in transgenic Nicotiana benthamiana using β-glucuronidase (GUS) as a reporter gene. GUS activity analyses revealed that during Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) infections, pPaAGO5b activity was significantly increased compared to pPaAGO5a and pPaAGO5c. Analysis of pPaAGO5b 5′-deletion revealed that pPaAGO5b_941 has higher activity during virus infection. Further, yeast one-hybrid analysis showed that the transcription factor NbMYB30 physically interacted with pPaAGO5b_941 to enhance its activity. Overexpression and silencing of NbMYB30 resulted in up- and downregulation of GUS expression, respectively. Exogenous application and endogenous measurement of phytohormones have shown that methyl jasmonate and salicylic acid respond to viral infections. NbMYB30 overexpression and its closest related protein, PaMYB30, in P. aphrodite subsp. formosana reduced CymMV accumulation in P. aphrodite subsp. formosana. Based on these discoveries, this study uncovers the interaction between virus-responsive promoter and the corresponding transcription factor in plants.
AbstractList Plant ARGONAUTES (AGOs) play a significant role in the defense against viral infection. Previously, we have demonstrated that AGO5s encoded in Phalaenopsis aphrodite subsp. formosana (PaAGO5s) took an indispensable part in defense against major viruses. To understand the underlying defense mechanism, we cloned PaAGO5s promoters (pPaAGO5s) and analyzed their activity in transgenic Nicotiana benthamiana using β-glucuronidase (GUS) as a reporter gene. GUS activity analyses revealed that during Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) infections, pPaAGO5b activity was significantly increased compared to pPaAGO5a and pPaAGO5c. Analysis of pPaAGO5b 5′-deletion revealed that pPaAGO5b_941 has higher activity during virus infection. Further, yeast one-hybrid analysis showed that the transcription factor NbMYB30 physically interacted with pPaAGO5b_941 to enhance its activity. Overexpression and silencing of NbMYB30 resulted in up- and downregulation of GUS expression, respectively. Exogenous application and endogenous measurement of phytohormones have shown that methyl jasmonate and salicylic acid respond to viral infections. NbMYB30 overexpression and its closest related protein, PaMYB30, in P. aphrodite subsp. formosana reduced CymMV accumulation in P. aphrodite subsp. formosana. Based on these discoveries, this study uncovers the interaction between virus-responsive promoter and the corresponding transcription factor in plants.
Plant ARGONAUTES (AGOs) play a significant role in the defense against viral infection. Previously, we have demonstrated that AGO5s encoded in subsp. (PaAGO5s) took an indispensable part in defense against major viruses. To understand the underlying defense mechanism, we cloned PaAGO5s promoters ( PaAGO5s) and analyzed their activity in transgenic using β-glucuronidase (GUS) as a reporter gene. GUS activity analyses revealed that during (CymMV) and (ORSV) infections, PaAGO5b activity was significantly increased compared to PaAGO5a and PaAGO5c. Analysis of PaAGO5b 5'-deletion revealed that PaAGO5b_941 has higher activity during virus infection. Further, yeast one-hybrid analysis showed that the transcription factor NbMYB30 physically interacted with PaAGO5b_941 to enhance its activity. Overexpression and silencing of resulted in up- and downregulation of expression, respectively. Exogenous application and endogenous measurement of phytohormones have shown that methyl jasmonate and salicylic acid respond to viral infections. NbMYB30 overexpression and its closest related protein, PaMYB30, in subsp. reduced CymMV accumulation in subsp. . Based on these discoveries, this study uncovers the interaction between virus-responsive promoter and the corresponding transcription factor in plants.
Plant ARGONAUTES (AGOs) play a significant role in the defense against viral infection. Previously, we have demonstrated that AGO5s encoded in Phalaenopsis aphrodite subsp. formosana (PaAGO5s) took an indispensable part in defense against major viruses. To understand the underlying defense mechanism, we cloned PaAGO5s promoters ( p PaAGO5s) and analyzed their activity in transgenic Nicotiana benthamiana using β-glucuronidase (GUS) as a reporter gene. GUS activity analyses revealed that during Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) infections, p PaAGO5b activity was significantly increased compared to p PaAGO5a and p PaAGO5c. Analysis of p PaAGO5b 5′-deletion revealed that p PaAGO5b_941 has higher activity during virus infection. Further, yeast one-hybrid analysis showed that the transcription factor NbMYB30 physically interacted with p PaAGO5b_941 to enhance its activity. Overexpression and silencing of NbMYB30 resulted in up- and downregulation of GUS expression, respectively. Exogenous application and endogenous measurement of phytohormones have shown that methyl jasmonate and salicylic acid respond to viral infections. NbMYB30 overexpression and its closest related protein, PaMYB30, in P. aphrodite subsp. formosana reduced CymMV accumulation in P. aphrodite subsp. formosana . Based on these discoveries, this study uncovers the interaction between virus-responsive promoter and the corresponding transcription factor in plants.
Plant ARGONAUTES (AGOs) play a significant role in the defense against viral infection. Previously, we have demonstrated that AGO5s encoded in Phalaenopsis aphrodite subsp. formosana (PaAGO5s) took an indispensable part in defense against major viruses. To understand the underlying defense mechanism, we cloned PaAGO5s promoters (pPaAGO5s) and analyzed their activity in transgenic Nicotiana benthamiana using β-glucuronidase (GUS) as a reporter gene. GUS activity analyses revealed that during Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) infections, pPaAGO5b activity was significantly increased compared to pPaAGO5a and pPaAGO5c. Analysis of pPaAGO5b 5'-deletion revealed that pPaAGO5b_941 has higher activity during virus infection. Further, yeast one-hybrid analysis showed that the transcription factor NbMYB30 physically interacted with pPaAGO5b_941 to enhance its activity. Overexpression and silencing of NbMYB30 resulted in up- and downregulation of GUS expression, respectively. Exogenous application and endogenous measurement of phytohormones have shown that methyl jasmonate and salicylic acid respond to viral infections. NbMYB30 overexpression and its closest related protein, PaMYB30, in P. aphrodite subsp. formosana reduced CymMV accumulation in P. aphrodite subsp. formosana. Based on these discoveries, this study uncovers the interaction between virus-responsive promoter and the corresponding transcription factor in plants.Plant ARGONAUTES (AGOs) play a significant role in the defense against viral infection. Previously, we have demonstrated that AGO5s encoded in Phalaenopsis aphrodite subsp. formosana (PaAGO5s) took an indispensable part in defense against major viruses. To understand the underlying defense mechanism, we cloned PaAGO5s promoters (pPaAGO5s) and analyzed their activity in transgenic Nicotiana benthamiana using β-glucuronidase (GUS) as a reporter gene. GUS activity analyses revealed that during Cymbidium mosaic virus (CymMV) and Odontoglossum ringspot virus (ORSV) infections, pPaAGO5b activity was significantly increased compared to pPaAGO5a and pPaAGO5c. Analysis of pPaAGO5b 5'-deletion revealed that pPaAGO5b_941 has higher activity during virus infection. Further, yeast one-hybrid analysis showed that the transcription factor NbMYB30 physically interacted with pPaAGO5b_941 to enhance its activity. Overexpression and silencing of NbMYB30 resulted in up- and downregulation of GUS expression, respectively. Exogenous application and endogenous measurement of phytohormones have shown that methyl jasmonate and salicylic acid respond to viral infections. NbMYB30 overexpression and its closest related protein, PaMYB30, in P. aphrodite subsp. formosana reduced CymMV accumulation in P. aphrodite subsp. formosana. Based on these discoveries, this study uncovers the interaction between virus-responsive promoter and the corresponding transcription factor in plants.
Author Kuo, Song-Yi
Huang, Ying-Wen
Kasi Viswanath, Kotapati
Tsao, Nai-Wen
Hsu, Yau-Heiu
Hu, Chung-Chi
Wang, Sheng-Yang
Lin, Na-Sheng
AuthorAffiliation 3 Department of Forestry, National Chung Hsing University, Taichung 40227, Taiwan
1 Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan
2 Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan
4 Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan
AuthorAffiliation_xml – name: 4 Institute of Plant and Microbial Biology, Academia Sinica, Taipei 11529, Taiwan
– name: 2 Advanced Plant Biotechnology Center, National Chung Hsing University, Taichung 40227, Taiwan
– name: 3 Department of Forestry, National Chung Hsing University, Taichung 40227, Taiwan
– name: 1 Graduate Institute of Biotechnology, National Chung Hsing University, Taichung 40227, Taiwan
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  surname: Kasi Viswanath
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Cites_doi 10.1111/nph.16048
10.1006/meth.2001.1262
10.1016/j.pbi.2018.05.007
10.1016/j.coviro.2018.12.003
10.1111/nph.14376
10.1007/s12298-013-0179-1
10.1038/s41598-018-28388-9
10.1111/j.1365-313X.2008.03778.x
10.1104/pp.20.00859
10.1073/pnas.0805760105
10.1007/s00344-019-10025-y
10.1111/j.1365-313X.2009.03832.x
10.1002/j.1460-2075.1987.tb02730.x
10.1093/nar/gky1081
10.1046/j.0960-7412.2000.00942.x
10.1016/j.plaphy.2017.09.020
10.1093/pcp/pcn043
10.1038/nprot.2010.37
10.1016/j.virol.2014.03.026
10.1007/s00425-005-0067-1
10.1111/pbi.12140
10.3732/apps.1600109
10.1105/tpc.104.027235
10.1104/pp.107.112029
10.1093/nar/30.1.325
10.1111/mpp.12868
10.1104/pp.16.00015
10.1073/pnas.93.25.14972
10.1094/MPMI-07-16-0147-R
10.3390/genes10070526
10.1105/tpc.15.00264
10.1371/journal.ppat.1002726
10.1111/j.1365-313X.2007.03039.x
10.3835/plantgenome2015.09.0082
10.1094/MPMI-11-13-0349-R
10.1080/15592324.2019.1596719
10.1128/JVI.00556-11
10.1111/tpj.15615
10.1104/pp.111.178764
10.7554/eLife.05733
10.1186/s12870-019-1918-4
10.1111/nph.16066
10.1111/mpp.13049
10.1101/SQB.1985.050.01.054
10.1111/tpj.14798
10.1111/mpp.12204
10.1016/j.plrev.2013.05.001
10.1007/978-1-0716-0751-0_2
10.1073/pnas.1804233115
10.1038/nature02874
10.1046/j.1365-313x.1998.00343.x
10.3390/ijms20102538
10.1016/j.scienta.2018.04.020
10.1371/journal.ppat.1009242
10.1186/1471-2229-9-152
10.1104/pp.113.229666
10.1016/j.tim.2019.05.007
10.1094/PD-74-0621
10.1146/annurev-phyto-080516-035544
10.1104/pp.111.188789
10.1007/BF00036968
10.1094/MPMI.1999.12.5.377
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Keywords CymMV
NbMYB30
Argonautes 5b
ORSV
MeJA
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These authors contributed equally to this work.
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References Ndamukong (ref_45) 2007; 50
Fichman (ref_42) 2020; 184
Chao (ref_34) 2017; 58
Bhattacharjee (ref_13) 2009; 58
ref_57
ref_12
Islam (ref_18) 2019; 14
Siegel (ref_50) 2017; 5
ref_53
Qu (ref_6) 2008; 105
Zhu (ref_47) 2014; 27
ref_19
Pan (ref_64) 2010; 5
He (ref_48) 2017; 214
Hartman (ref_54) 1989; 12
Li (ref_5) 2022; 109
Petchthai (ref_37) 2018; 8
Li (ref_3) 2019; 27
Cheng (ref_59) 2013; 163
Berens (ref_16) 2017; 55
Alazem (ref_17) 2015; 16
Ratcliff (ref_62) 2001; 25
Huang (ref_15) 2019; 224
Kuo (ref_33) 2021; 22
Li (ref_26) 2018; 237
Ambawat (ref_39) 2013; 19
Yang (ref_25) 1996; 93
Clough (ref_55) 1998; 16
Alazem (ref_8) 2017; 174
Prasanth (ref_58) 2011; 85
Jefferson (ref_60) 1987; 6
Li (ref_20) 2012; 158
Huang (ref_27) 2016; 9
Takeda (ref_14) 2008; 49
Koornneef (ref_46) 2008; 146
ref_36
ref_35
Mabuchi (ref_40) 2018; 115
Baulcombe (ref_2) 2004; 431
Zettler (ref_32) 1990; 74
Ma (ref_4) 2018; 45
Huang (ref_23) 2017; 120
Kotnik (ref_52) 2013; 10
Gayral (ref_30) 2020; 103
Selth (ref_22) 2005; 17
Liou (ref_56) 2014; 12
Lescot (ref_51) 2002; 30
Yang (ref_21) 2020; 28
McLeish (ref_1) 2019; 34
Ghoshal (ref_10) 2014; 456
Park (ref_28) 2006; 223
Meng (ref_31) 2020; 39
He (ref_49) 2020; 225
Sun (ref_24) 2019; 20
ref_44
ref_41
Geri (ref_43) 1999; 12
Livak (ref_63) 2001; 25
Brosseau (ref_7) 2015; 27
Scholthof (ref_11) 2011; 156
Wu (ref_9) 2015; 4
Gaguancela (ref_29) 2016; 29
Li (ref_38) 2009; 58
Chow (ref_61) 2019; 47
References_xml – volume: 224
  start-page: 804
  year: 2019
  ident: ref_15
  article-title: Nicotiana benthamiana Argonaute10 plays a pro-viral role in Bamboo mosaic virus infection
  publication-title: New Phytol.
  doi: 10.1111/nph.16048
– volume: 25
  start-page: 402
  year: 2001
  ident: ref_63
  article-title: Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method
  publication-title: Methods
  doi: 10.1006/meth.2001.1262
– volume: 45
  start-page: 59
  year: 2018
  ident: ref_4
  article-title: Actions of plant Argonautes: Predictable or unpredictable?
  publication-title: Curr. Opin. Plant Biol.
  doi: 10.1016/j.pbi.2018.05.007
– volume: 34
  start-page: 50
  year: 2019
  ident: ref_1
  article-title: Evolution of plant–virus interactions: Host range and virus emergence
  publication-title: Curr. Opin. Virol.
  doi: 10.1016/j.coviro.2018.12.003
– volume: 214
  start-page: 388
  year: 2017
  ident: ref_48
  article-title: Jasmonic acid-mediated defense suppresses brassinosteroid-mediated susceptibility to Rice black streaked dwarf virus infection in rice
  publication-title: New Phytol.
  doi: 10.1111/nph.14376
– volume: 19
  start-page: 307
  year: 2013
  ident: ref_39
  article-title: MYB transcription factor genes as regulators for plant responses: An overview
  publication-title: Physiol. Mol. Biol. Plants
  doi: 10.1007/s12298-013-0179-1
– volume: 8
  start-page: 9958
  year: 2018
  ident: ref_37
  article-title: Resistance to CymMV and ORSV in artificial microRNA transgenic Nicotiana benthamiana plants
  publication-title: Sci. Rep.
  doi: 10.1038/s41598-018-28388-9
– volume: 58
  start-page: 275
  year: 2009
  ident: ref_38
  article-title: Arabidopsis MYB30 is a direct target of BES1 and cooperates with BES1 to regulate brassinosteroid-induced gene expression
  publication-title: Plant J.
  doi: 10.1111/j.1365-313X.2008.03778.x
– volume: 184
  start-page: 666
  year: 2020
  ident: ref_42
  article-title: MYB30 orchestrates systemic reactive oxygen signaling and plant acclimation
  publication-title: Plant Physiol.
  doi: 10.1104/pp.20.00859
– volume: 105
  start-page: 14732
  year: 2008
  ident: ref_6
  article-title: Arabidopsis DRB4, AGO1, AGO7, and RDR6 participate in a DCL4-initiated antiviral RNA silencing pathway negatively regulated by DCL1
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.0805760105
– volume: 39
  start-page: 823
  year: 2020
  ident: ref_31
  article-title: Comparative metabolomic and transcriptome analysis reveal distinct flavonoid biosynthesis regulation between petals of white and purple Phalaenopsis amabilis
  publication-title: J. Plant Growth Regul.
  doi: 10.1007/s00344-019-10025-y
– volume: 58
  start-page: 940
  year: 2009
  ident: ref_13
  article-title: Virus resistance induced by NB-LRR proteins involves Argonaute4-dependent translational control
  publication-title: Plant J.
  doi: 10.1111/j.1365-313X.2009.03832.x
– volume: 6
  start-page: 3901
  year: 1987
  ident: ref_60
  article-title: GUS fusions: Beta-glucuronidase as a sensitive and versatile gene fusion marker in higher plants
  publication-title: EMBO J.
  doi: 10.1002/j.1460-2075.1987.tb02730.x
– volume: 47
  start-page: D1155
  year: 2019
  ident: ref_61
  article-title: PlantPAN3. 0: A new and updated resource for reconstructing transcriptional regulatory networks from ChIP-seq experiments in plants
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/gky1081
– volume: 25
  start-page: 237
  year: 2001
  ident: ref_62
  article-title: Technical advance: Tobacco rattle virus as a vector for analysis of gene function by silencing
  publication-title: Plant J.
  doi: 10.1046/j.0960-7412.2000.00942.x
– volume: 120
  start-page: 61
  year: 2017
  ident: ref_23
  article-title: Six NAC transcription factors involved in response to TYLCV infection in resistant and susceptible tomato cultivars
  publication-title: Plant Physiol. Biochem.
  doi: 10.1016/j.plaphy.2017.09.020
– volume: 49
  start-page: 493
  year: 2008
  ident: ref_14
  article-title: The mechanism selecting the guide strand from small RNA duplexes is different among argonaute proteins
  publication-title: Plant Cell Physiol.
  doi: 10.1093/pcp/pcn043
– volume: 5
  start-page: 986
  year: 2010
  ident: ref_64
  article-title: Quantitative analysis of major plant hormones in crude plant extracts by high-performance liquid chromatography–mass spectrometry
  publication-title: Nat. Protoc.
  doi: 10.1038/nprot.2010.37
– volume: 456
  start-page: 188
  year: 2014
  ident: ref_10
  article-title: Temperature-dependent symptom recovery in Nicotiana benthamiana plants infected with Tomato ringspot virus is associated with reduced translation of viral RNA2 and requires ARGONAUTE 1
  publication-title: Virology
  doi: 10.1016/j.virol.2014.03.026
– volume: 223
  start-page: 168
  year: 2006
  ident: ref_28
  article-title: A hot pepper gene encoding WRKY transcription factor is induced during hypersensitive response to Tobacco mosaic virus and Xanthomonas campestris
  publication-title: Planta
  doi: 10.1007/s00425-005-0067-1
– volume: 12
  start-page: 330
  year: 2014
  ident: ref_56
  article-title: A dual gene-silencing vector system for monocot and dicot plants
  publication-title: Plant Biotechnol. J.
  doi: 10.1111/pbi.12140
– volume: 5
  start-page: 1600109
  year: 2017
  ident: ref_50
  article-title: Evaluation and comparison of FTA card and CTAB DNA extraction methods for non-agricultural taxa
  publication-title: Appl. Plant Sci.
  doi: 10.3732/apps.1600109
– volume: 17
  start-page: 311
  year: 2005
  ident: ref_22
  article-title: A NAC domain protein interacts with Tomato leaf curl virus replication accessory protein and enhances viral replication
  publication-title: Plant Cell
  doi: 10.1105/tpc.104.027235
– volume: 146
  start-page: 839
  year: 2008
  ident: ref_46
  article-title: Cross talk in defense signaling
  publication-title: Plant Physiol.
  doi: 10.1104/pp.107.112029
– volume: 30
  start-page: 325
  year: 2002
  ident: ref_51
  article-title: PlantCARE, a database of plant cis-acting regulatory elements and a portal to tools for in silico analysis of promoter sequences
  publication-title: Nucleic Acids Res.
  doi: 10.1093/nar/30.1.325
– volume: 20
  start-page: 1662
  year: 2019
  ident: ref_24
  article-title: Comparative transcriptome profiling uncovers a Lilium regale NAC transcription factor, LrNAC35, contributing to defence response against Cucumber mosaic virus and Tobacco mosaic virus
  publication-title: Mol. Plant Pathol.
  doi: 10.1111/mpp.12868
– volume: 174
  start-page: 339
  year: 2017
  ident: ref_8
  article-title: Abscisic acid induces resistance against bamboo mosaic virus through Argonaute 2 and 3
  publication-title: Plant Physiol.
  doi: 10.1104/pp.16.00015
– volume: 93
  start-page: 14972
  year: 1996
  ident: ref_25
  article-title: Isolation and characterization of a Tobacco mosaic virus-inducible myb oncogene homolog from tobacco
  publication-title: Proc. Natl. Acad. Sci. UAS
  doi: 10.1073/pnas.93.25.14972
– volume: 29
  start-page: 750
  year: 2016
  ident: ref_29
  article-title: The IRE1/bZIP60 pathway and bax inhibitor 1 suppress systemic accumulation of potyviruses and potexviruses in Arabidopsis and Nicotiana benthamiana plants
  publication-title: Mol. Plant-Microbe Interact.
  doi: 10.1094/MPMI-07-16-0147-R
– ident: ref_12
  doi: 10.3390/genes10070526
– volume: 27
  start-page: 1742
  year: 2015
  ident: ref_7
  article-title: Functional and genetic analysis identify a role for Arabidopsis ARGONAUTE5 in antiviral RNA silencing
  publication-title: Plant Cell
  doi: 10.1105/tpc.15.00264
– ident: ref_57
  doi: 10.1371/journal.ppat.1002726
– volume: 50
  start-page: 128
  year: 2007
  ident: ref_45
  article-title: SA-inducible Arabidopsis glutaredoxin interacts with TGA factors and suppresses JA-responsive PDF1. 2 transcription
  publication-title: Plant J.
  doi: 10.1111/j.1365-313X.2007.03039.x
– volume: 9
  start-page: plantgenome2015.09.0082
  year: 2016
  ident: ref_27
  article-title: AP2/ERF transcription factors involved in response to Tomato yellow leaf curly virus in tomato
  publication-title: Plant Genome
  doi: 10.3835/plantgenome2015.09.0082
– volume: 27
  start-page: 567
  year: 2014
  ident: ref_47
  article-title: Salicylic acid and jasmonic acid are essential for systemic resistance against Tobacco mosaic virus in Nicotiana benthamiana
  publication-title: Mol. Plant-Microbe Interact.
  doi: 10.1094/MPMI-11-13-0349-R
– volume: 14
  start-page: 1596719
  year: 2019
  ident: ref_18
  article-title: Plant defense against virus diseases; growth hormones in highlights
  publication-title: Plant Signal. Behav.
  doi: 10.1080/15592324.2019.1596719
– volume: 85
  start-page: 8829
  year: 2011
  ident: ref_58
  article-title: Glyceraldehyde 3-phosphate dehydrogenase negatively regulates the replication of Bamboo mosaic virus and its associated satellite RNA
  publication-title: J. Virol.
  doi: 10.1128/JVI.00556-11
– volume: 109
  start-page: 1086
  year: 2022
  ident: ref_5
  article-title: Origin, evolution and diversification of plant ARGONAUTE proteins
  publication-title: Plant J.
  doi: 10.1111/tpj.15615
– volume: 156
  start-page: 1548
  year: 2011
  ident: ref_11
  article-title: Identification of an ARGONAUTE for antiviral RNA silencing in Nicotiana benthamiana
  publication-title: Plant Physiol.
  doi: 10.1104/pp.111.178764
– volume: 4
  start-page: e05733
  year: 2015
  ident: ref_9
  article-title: Viral-inducible Argonaute18 confers broad-spectrum virus resistance in rice by sequestering a host microRNA
  publication-title: eLife
  doi: 10.7554/eLife.05733
– ident: ref_41
  doi: 10.1186/s12870-019-1918-4
– volume: 225
  start-page: 896
  year: 2020
  ident: ref_49
  article-title: Rice black-streaked dwarf virus-encoded P5-1 regulates the ubiquitination activity of SCF E3 ligases and inhibits jasmonate signaling to benefit its infection in rice
  publication-title: New Phytol.
  doi: 10.1111/nph.16066
– volume: 22
  start-page: 627
  year: 2021
  ident: ref_33
  article-title: Argonaute 5 family proteins play crucial roles in the defence against Cymbidium mosaic virus and Odontoglossum ringspot virus in Phalaenopsis aphrodite subsp. formosana
  publication-title: Mol. Plant Pathol.
  doi: 10.1111/mpp.13049
– ident: ref_53
  doi: 10.1101/SQB.1985.050.01.054
– volume: 103
  start-page: 1233
  year: 2020
  ident: ref_30
  article-title: Multiple ER-to-nucleus stress signaling pathways are activated during Plantago asiatica mosaic virus and Turnip mosaic virus infection in Arabidopsis thaliana
  publication-title: Plant J.
  doi: 10.1111/tpj.14798
– volume: 16
  start-page: 529
  year: 2015
  ident: ref_17
  article-title: Roles of plant hormones in the regulation of host-virus interactions
  publication-title: Mol. Plant Pathol.
  doi: 10.1111/mpp.12204
– volume: 10
  start-page: 351
  year: 2013
  ident: ref_52
  article-title: Lightning-triggered electroporation and electrofusion as possible contributors to natural horizontal gene transfer
  publication-title: Phys. Life Rev.
  doi: 10.1016/j.plrev.2013.05.001
– ident: ref_35
  doi: 10.1007/978-1-0716-0751-0_2
– volume: 115
  start-page: E4710
  year: 2018
  ident: ref_40
  article-title: MYB30 links ROS signaling, root cell elongation, and plant immune responses
  publication-title: Proc. Natl. Acad. Sci. USA
  doi: 10.1073/pnas.1804233115
– volume: 431
  start-page: 356
  year: 2004
  ident: ref_2
  article-title: RNA silencing in plants
  publication-title: Nature
  doi: 10.1038/nature02874
– volume: 16
  start-page: 735
  year: 1998
  ident: ref_55
  article-title: Floral dip: A simplified method for Agrobacterium-mediated transformation of Arabidopsis thaliana
  publication-title: Plant J.
  doi: 10.1046/j.1365-313x.1998.00343.x
– ident: ref_19
  doi: 10.3390/ijms20102538
– volume: 237
  start-page: 192
  year: 2018
  ident: ref_26
  article-title: An R2R3-MYB transcription factor, SlMYB28, involved in the regulation of TYLCV infection in tomato
  publication-title: Sci. Hortic.
  doi: 10.1016/j.scienta.2018.04.020
– ident: ref_44
  doi: 10.1371/journal.ppat.1009242
– ident: ref_36
  doi: 10.1186/1471-2229-9-152
– volume: 163
  start-page: 1598
  year: 2013
  ident: ref_59
  article-title: Chloroplast phosphoglycerate kinase is involved in the targeting of Bamboo mosaic virus to chloroplasts in Nicotiana benthamiana plants
  publication-title: Plant Physiol.
  doi: 10.1104/pp.113.229666
– volume: 27
  start-page: 792
  year: 2019
  ident: ref_3
  article-title: RNA-targeted antiviral immunity: More than just RNA silencing
  publication-title: Trends Microbiol.
  doi: 10.1016/j.tim.2019.05.007
– volume: 58
  start-page: e9
  year: 2017
  ident: ref_34
  article-title: Orchidstra 2.0-a transcriptomics resource for the orchid family
  publication-title: Plant Cell Physiol.
– volume: 74
  start-page: 621
  year: 1990
  ident: ref_32
  article-title: Viruses of orchids and their control
  publication-title: Plant Dis.
  doi: 10.1094/PD-74-0621
– volume: 28
  start-page: 89
  year: 2020
  ident: ref_21
  article-title: Jasmonate signaling enhances RNA silencing and antiviral defense in rice
  publication-title: Proc. Natl. Acad. Sci. USA
– volume: 55
  start-page: 401
  year: 2017
  ident: ref_16
  article-title: Evolution of hormone signaling networks in plant defense
  publication-title: Annu. Rev. Phytopathol.
  doi: 10.1146/annurev-phyto-080516-035544
– volume: 158
  start-page: 1279
  year: 2012
  ident: ref_20
  article-title: Transcriptional regulation of Arabidopsis miR168a and argonaute1 homeostasis in abscisic acid and abiotic stress responses
  publication-title: Plant Physiol.
  doi: 10.1104/pp.111.188789
– volume: 12
  start-page: 539
  year: 1989
  ident: ref_54
  article-title: Regulation of chalcone flavanone isomerase (CHI) gene expression in Petunia hybrida: The use of alternative promoters in corolla, anthers and pollen
  publication-title: Plant Mol. Biol.
  doi: 10.1007/BF00036968
– volume: 12
  start-page: 377
  year: 1999
  ident: ref_43
  article-title: Altered patterns of gene expression in Arabidopsis elicited by cauliflower mosaic virus (CaMV) infection and by a CaMV gene VI transgene
  publication-title: Mol. Plant-Microbe Interact.
  doi: 10.1094/MPMI.1999.12.5.377
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Snippet Plant ARGONAUTES (AGOs) play a significant role in the defense against viral infection. Previously, we have demonstrated that AGO5s encoded in Phalaenopsis...
Plant ARGONAUTES (AGOs) play a significant role in the defense against viral infection. Previously, we have demonstrated that AGO5s encoded in subsp. (PaAGO5s)...
Plant ARGONAUTES (AGOs) play a significant role in the defense against viral infection. Previously, we have demonstrated that AGO5s encoded in Phalaenopsis...
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SourceType Open Access Repository
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StartPage 9825
SubjectTerms Abscisic acid
Cloning
Gene expression
Infections
Nicotiana - genetics
Plants
Potexvirus - genetics
Proteins
Transcription Factors
Viral infections
Virus Diseases
Viruses
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Title Characterization of Virus-Inducible Orchid Argonaute 5b Promoter and Its Functional Characterization in Nicotiana benthamiana during Virus Infection
URI https://www.ncbi.nlm.nih.gov/pubmed/36077222
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Volume 23
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