DNA Polymerase-Parental DNA Interaction Is Essential for Helicase-Polymerase Coupling during Bacteriophage T7 DNA Replication

• Published in: International journal of molecular sciences Vol. 23; no. 3; p. 1342
• Main Authors: Lo, Chen-Yu, Gao, Yang
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 25.01.2022; MDPI
• Subjects: Bacteriophage T7 - enzymology; Bacteriophage T7 - metabolism; DNA Helicases - metabolism; DNA Helicases - physiology; DNA polymerase; DNA Replication - genetics; DNA Replication - physiology; DNA, Viral - metabolism; DNA-Directed DNA Polymerase - metabolism; DNA-Directed DNA Polymerase - physiology; Proteins; Viral Proteins - metabolism; DNA replication; helicase; bacteriophage T7; polymerase

DNA helicase and polymerase work cooperatively at the replication fork to perform leading-strand DNA synthesis. It was believed that the helicase migrates to the forefront of the replication fork where it unwinds the duplex to provide templates for DNA polymerases. However, the molecular basis of th...