Immunoproteomic characterization of Brucella abortus 1119-3 preparations used for the serodiagnosis of Brucella infections

• Published in: Journal of immunological methods Vol. 309; no. 1; pp. 34 - 47
• Main Authors: Al Dahouk, Sascha, Nöckler, Karsten, Scholz, Holger C., Tomaso, Herbert, Bogumil, Ralf, Neubauer, Heinrich
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 20.02.2006; Elsevier
• Subjects: Animals; Antigens, Bacterial - isolation & purification; Bacteriological methods and techniques used in bacteriology; Bacteriology; Biological and medical sciences; Brucella; Brucella abortus; Brucella abortus - immunology; Brucellosis - diagnosis; Brucellosis - immunology; Brucellosis - microbiology; Brucellosis - veterinary; Cross Reactions; Cross-reactivity; Electrophoresis, Gel, Two-Dimensional; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunogenic proteins; Immunoproteomics; Microbiology; Molecular immunology; Proteomics - methods; SELDI-MS; Serologic Tests - methods; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Techniques; MOMP; SPA; Cross-reactivity; Immunoproteomics; Brucella; IAA; LVS; SELDI-MS; r/s-LPS; CP; SELDI; Immunogenic proteins; Brucella abortus; Protein; Immunological method; Infection; Brucellaceae; Immunogenicity; Cross reaction; Bacteriosis; Bacteria; Brucellosis; Brucella melitensis
• ISSN: 0022-1759; 1872-7905
• DOI: 10.1016/j.jim.2005.11.003

The diagnosis of brucellosis is mainly based on the detection of anti-LPS antibodies. Due to substantial similarity of the O-polysaccharide of Brucella LPS to that of various other Gram-negative bacteria, serological tests of samples containing high amounts of LPS lack specificity. Hence, the develo...